       Document 0512
 DOCN  M9620512
 TI    HIV-1 spreads from lymphocytes to normal human keratinocytes suitable
       for autologous and allogenic transplantation.
 DT    9602
 AU    Ramarli D; Giri A; Reina S; Poffe O; Cancedda R; Varnier O; Tridente G;
       De Luca M; Institute of Immunology and Infectious Diseases, University
       of; Verona, Italy.
 SO    J Invest Dermatol. 1995 Nov;105(5):644-7. Unique Identifier : AIDSLINE
       MED/96067276
 AB    Normal human keratinocytes can reconstitute in vitro cohesive sheets of
       epithelium suitable for grafting onto patients. Despite the widespread
       use of autografts and allografts, no data are yet available on
       productive infection by human immunodeficiency virus (HIV-1) of human
       keratinocytes. To address this point, we challenged keratinocytes at the
       second passage of culture with HTLV-IIIB virus by cell-free and
       cell-mediated inoculum. Viral entry was not achieved by cell-free
       inoculum, thus demonstrating that cultured keratinocytes do not provide
       the membrane requirements for viral binding and/or internalization. By
       contrast, the cell-mediated inoculum overcame specific receptor
       constraints, leading to viral integration and productive infection. The
       p24gag viral protein was transiently released in the culture
       supernatant, although at low level. The viral progeny produced by
       infected keratinocytes was rescued and amplified by co-culture
       experiments performed with the HIV-1 high sensitive CEM-SS human T-cell
       line. Viral integration, p24gag production, and secondary transmission
       to lymphoid cells was further confirmed with keratinocytes infected at
       the fourth passage of culture. Taken together, our results demonstrate
       that cultured keratinocytes can be infected by HTLV-IIIB virus, which
       can be maintained in semi-latent form for several passages after
       inoculum and rescued to full replication by a proper target. The in
       vitro demonstration of lympho-epithelial HIV-1 spreadings warns against
       the use of inappropriately screened biopsies for the preparation of skin
       grafts.
 DE    Acquired Immunodeficiency Syndrome/*TRANSMISSION  Cell Line  Cell
       Transplantation  Cell-Free System  Human  HIV Core Protein
       p24/METABOLISM  *HIV-1/PHYSIOLOGY
       Keratinocytes/*TRANSPLANTATION/*VIROLOGY  Lymphocytes/*VIROLOGY
       Support, Non-U.S. Gov't  Transplantation, Autologous  Transplantation,
       Homologous  Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

