       Document 0462
 DOCN  M9620462
 TI    Early replication block of human immunodeficiency virus type 1 in monkey
       cells.
 DT    9602
 AU    Shibata R; Sakai H; Kawamura M; Tokunaga K; Adachi A; Department of
       Viral Oncology, Kyoto University, Japan.
 SO    J Gen Virol. 1995 Nov;76 ( Pt 11):2723-30. Unique Identifier : AIDSLINE
       MED/96068805
 AB    The genetic and functional basis of the replication-defective nature of
       human immunodeficiency virus type 1 (HIV-1) in monkey cells was studied.
       By the generation and characterization of chimeras between HIV-1 and
       simian immunodeficiency virus, the sequence encompassing the 3' half of
       the long terminal repeat, gag and pol genes of HIV-1 was found to be
       responsible for the growth restriction. Early and late phases of HIV-1
       replication in monkey cells were analysed in detail using several assay
       systems: transfection/coculture, transcomplementation between various
       proviral clones carrying the CAT gene and effector clones and evaluation
       of transcription and reverse transcription. All the data were consistent
       with the notion that HIV-1 replication is blocked at a very early
       stage(s) such as uncoating and/or reverse transcription in monkey cells.
 DE    Animal  Base Sequence  Cells, Cultured  Defective
       Viruses/GENETICS/PHYSIOLOGY  DNA Primers  DNA, Viral/*BIOSYNTHESIS
       Genes, gag  Genes, pol  Haplorhini  Human  *HIV Long Terminal Repeat
       HIV-1/GENETICS/*PHYSIOLOGY  Leukocytes, Mononuclear/CYTOLOGY/VIROLOGY
       Molecular Sequence Data  Recombination, Genetic  Support, Non-U.S. Gov't
       SIV/GENETICS  Transcription, Genetic  *Virus Replication  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

