       Document 0420
 DOCN  M9620420
 TI    Nucleotide changes in oxidatively stressed lymphocytes.
 DT    9602
 AU    Palace GP; Lawrence DA; Department of Pharmacology and Toxicology,
       Albany Medical; College, NY 12208, USA.
 SO    J Biochem Toxicol. 1995 Jun;10(3):137-42. Unique Identifier : AIDSLINE
       MED/96073320
 AB    Similar to HIV-1-induced suppression of thymus-derived lymphocytes (T
       cells), oxidatively stressed T cells show inhibited DNA synthesis and
       proliferation. The influence of oxidative stress on nucleotide pools was
       explored using 3H-uridine addition to OKT3-stimulated peripheral blood
       lymphocytes. The cells were preincubated and stimulated in the presence
       of 1 mM buthionine sulfoximine to inhibit GSH synthesis. This treatment
       gave rise to a significant reduction in dUDP and TTP biosynthesis
       following 18-32 hours stimulation, indicating possible impairment of
       ribonucleotide reductase activity.
 DE    Cell Cycle  Cells, Cultured  Chromatography, High Pressure Liquid
       Deoxyuracil Nucleotides/BLOOD  Enzyme Inhibitors/PHARMACOLOGY
       Glutathione/BLOOD  Human  Lymphocyte Transformation
       Lymphocytes/IMMUNOLOGY/*METABOLISM  Methionine Sulfoximine/ANALOGS &
       DERIVATIVES/PHARMACOLOGY  Muromonab-CD3/IMMUNOLOGY  Nucleotides/*BLOOD
       Oxidative Stress/*PHYSIOLOGY  Support, U.S. Gov't, P.H.S.  Thymine
       Nucleotides/BLOOD  Uridine/BLOOD  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

