       Document 0401
 DOCN  M9620401
 TI    Immunogenic determinants of a neuropathogenic murine leukemia virus.
 DT    9602
 AU    Robbins DS; Remington MP; Sarzotti M; St. Louis D; Hoffman PM; Research
       Service, Department of Veterans Affairs Medical Center,; Baltimore,
       Maryland 21201, USA.
 SO    J Virol. 1995 Nov;69(11):6847-51. Unique Identifier : AIDSLINE
       MED/96013781
 AB    Previous studies of Cas-Br-M murine leukemia virus (MuLV) (Cas-MuLV)
       infection demonstrated that cytotoxic T cells (CTL) of the CD8+
       phenotype play a role in resistance to the neuropathogenic effects of
       the virus in NFS/N mice. In the current study, we sought to identify the
       Cas-MuLV epitopes that are immunogenic for the CTL response. Infection
       of adult NFS/N mice with a well-characterized neuropathogenic variant of
       Friend MuLV, PVC-211 MuLV (PVC-MuLV), was not immunogenic for
       MuLV-specific CTL. Therefore, we constructed chimeric viruses between
       Cas-MuLV and PVC-MuLV. Infectious chimeras contained the Cas-MuLV env
       gene on a PVC-MuLV background (PVC-CasenvMuLV) and the PVC-MuLV env gene
       on a Cas-MuLV background (Cas-PVCenvMuLV). Cas-MuLV-specific CTL were
       found following inoculation of both the chimeric viruses and the
       parental Cas-MuLV but not the parental PVC-MuLV, despite evidence of
       antibody responses to both parental and chimeric MuLV. CTL generated in
       response to infection with PVC-CasenvMuLV and Cas-PVCenvMuLV were
       exclusively of the CD8+ phenotype. These results indicate that both the
       env and gag-pol regions of Cas-MuLV express epitopes that are
       immunogenic for CTL.
 DE    Animal  Antibody Formation  Antigens, Viral/ANALYSIS  Chimera
       Cytotoxicity, Immunologic  CD8-Positive T-Lymphocytes/*IMMUNOLOGY
       Enzyme-Linked Immunosorbent Assay  Epitopes/*ANALYSIS  Female  Friend
       Virus/IMMUNOLOGY/PATHOGENICITY  Genes, env  Genes, gag  Genes, pol
       Immunity, Natural  Killer Cells/IMMUNOLOGY  Leukemia Viruses,
       Murine/GENETICS/*IMMUNOLOGY/*PATHOGENICITY  Mice  Mice, Inbred Strains
       Nervous System/*VIROLOGY  Pregnancy  Support, U.S. Gov't, Non-P.H.S.
       T-Lymphocytes, Cytotoxic/*IMMUNOLOGY  3T3 Cells  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

