       Document 0398
 DOCN  M9620398
 TI    A leucine triplet repeat sequence (LXX)4 in p6gag is important for Vpr
       incorporation into human immunodeficiency virus type 1 particles.
 DT    9602
 AU    Lu YL; Bennett RP; Wills JW; Gorelick R; Ratner L; Department of
       Medicine, Washington University, St. Louis,; Missouri 63110, USA.
 SO    J Virol. 1995 Nov;69(11):6873-9. Unique Identifier : AIDSLINE
       MED/96013785
 AB    Incorporation of Vpr into human immunodeficiency virus type 1 (HIV-1)
       virions is mediated by the Gag protein, independently of other viral
       components. We have coexpressed Vpr and Gag constructs in a vaccinia
       virus expression system in order to map the region of Gag involved in
       Vpr packaging. Deletion of the carboxyl-terminal p6 region of Gag
       impaired the ability of Gag to package Vpr. To confirm the role of p6 in
       Vpr packaging, Rous sarcoma virus (RSV)-HIV chimeras containing HIV-1 p6
       were constructed. Although RSV Gag does not package Vpr into virus
       particles, a chimera containing HIV-1 p6 is sufficient for Vpr
       incorporation. To map the region of p6 involved in Vpr packaging, a
       series of p6 point mutations and deletion mutations was analyzed.
       Mutations in the N-terminal p6 proline-rich domain, for which
       preliminary evidence shows a marked decrease in virion incorporated RNA,
       did not affect Vpr incorporation. Deletion of residues 1 to 31 of HIV-1
       p6 did not affect Vpr packaging, but residues 35 to 47, including an
       (LXX)4 domain, were required for Vpr incorporation into virus particles.
 DE    Amino Acid Sequence  Animal  Base Sequence  Cell Line  Comparative Study
       DNA Primers  Gene Expression  Gene Products,
       gag/BIOSYNTHESIS/*METABOLISM  Gene Products,
       vpr/BIOSYNTHESIS/*METABOLISM  *Genes, gag  Human
       HIV-1/GENETICS/*METABOLISM  Leucine  Molecular Sequence Data
       Mutagenesis, Site-Directed  Plasmids  Polymerase Chain Reaction
       Recombinant Proteins/BIOSYNTHESIS/METABOLISM  Support, Non-U.S. Gov't
       Support, U.S. Gov't, P.H.S.  Vaccinia Virus  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

