       Document 0397
 DOCN  M9620397
 TI    Functional epitope analysis of the human CD4 molecule: antibodies that
       inhibit human immunodeficiency virus type 1 gene expression bind to the
       immunoglobulin CDR3-like region of CD4.
 DT    9602
 AU    Benkirane M; Hirn M; Carriere D; Devaux C; Laboratoire d'Immunologie des
       Infections Retrovirales, Centre; National de la Recherche Scientifique
       UPR 9008-Institut National; de la Sante et de la Recherche Medicale
       U249, Montpellier,; France.
 SO    J Virol. 1995 Nov;69(11):6898-903. Unique Identifier : AIDSLINE
       MED/96013789
 AB    We recently demonstrated that monoclonal antibody (MAb) 13B8-2, specific
       for the immunoglobulin (Ig) complementary determining region 3
       (CDR3)-like region of the CD4 molecule, inhibits viral transcription in
       human immunodeficiency virus (HIV)-infected CEM cells and HIV type 1
       (HIV-1) promoter activity. Here, we have studied the capacity of several
       MAb specific for the D1 domain of CD4, including anti-CDR2-like (Leu-3a
       and ST4) and anti-CDR3-like (13B8-2 and ST40) MAb, and for the D2 domain
       of CD4 (BL4) to inhibit both provirus transcription in HIV-1LAI-infected
       CEM cells and transcription of the chloramphenicol acetyltransferase
       (CAT) gene under control of the HIV-1 long terminal repeat in
       transiently transfected CEM cells. We found that HIV-1 promoter activity
       and provirus transcription are inhibited only by MAb that bind to the
       CDR3-like region in domain 1 of CD4. Moreover, we demonstrated that the
       Fab fragment of an anti-CDR3-like region-specific anti-CD4 MAb is a
       powerful inhibitor of HIV-1 promoter activity. These results have
       implications for understanding the role of the CDR3-like region in CD4
       T-cell signaling, which controls provirus transcription.
 DE    Antibodies, Monoclonal/IMMUNOLOGY/*PHARMACOLOGY  Antigens,
       CD4/*IMMUNOLOGY/PHYSIOLOGY  Binding Sites  Cell Line  Chloramphenicol
       Acetyltransferase/BIOSYNTHESIS  Epitopes/*ANALYSIS  *Gene Expression
       Human  *HIV Long Terminal Repeat  HIV-1/*GENETICS/*IMMUNOLOGY
       Polymerase Chain Reaction  Receptor-CD3 Complex, Antigen,
       T-Cell/*IMMUNOLOGY/PHYSIOLOGY  Recombinant Proteins/BIOSYNTHESIS  RNA
       Splicing  RNA, Viral/BIOSYNTHESIS  Support, Non-U.S. Gov't  Transfection
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

