       Document 0380
 DOCN  M9620380
 TI    Human T-lymphotropic virus type 1 in coastal natives of British
       Columbia: phylogenetic affinities and possible origins.
 DT    9602
 AU    Picard FJ; Coulthart MB; Oger J; King EE; Kim S; Arp J; Rice GP; Dekaban
       GA; Gene Therapy and Molecular Virology Group, John P. Robarts; Research
       Institute, London, Ontario, Canada.
 SO    J Virol. 1995 Nov;69(11):7248-56. Unique Identifier : AIDSLINE
       GENBANK/U32558
 AB    Human T-lymphotropic virus type 1 (HTLV-1) infection has been discovered
       recently in people of Amerindian descent living in coastal areas of
       British Columbia, Canada. DNA sequencing combined with phylogenetic
       analysis and restriction fragment length polymorphism (RFLP) typing of
       HTLV-1 strains recovered from these British Columbia Indians (BCI) was
       conducted. Sequence-based phylogenetic trees distributed the BCI
       isolates among the Japanese subcluster (subcluster B) and the
       geographically widely distributed subcluster (subcluster A) of the large
       HTLV-1 cosmopolitan cluster. Long terminal repeat (LTR) RFLP typing
       revealed three distinct, equally frequent LTR cleavage patterns, two of
       which were of previously recognized Japanese and widely dispersed
       cosmopolitan types. A third, new cleavage pattern was detected which may
       have arisen by recombination between two other HTLV-1 genotypes. Our
       results suggest multiple origins for HTLV-1 in BCI, which are equally
       consistent with (i) a cluster of recent sporadic infections, (ii)
       ancient endemic vertical transmission through Amerindian lineages, or
       (iii) both.
 DE    Base Sequence  British Columbia/EPIDEMIOLOGY  Comparative Study
       Demography  DNA Primers  Human
       HTLV-I/*CLASSIFICATION/*GENETICS/ISOLATION & PURIF  HTLV-I
       Infections/EPIDEMIOLOGY/*VIROLOGY  Indians, North American  Molecular
       Sequence Data  *Phylogeny  Polymerase Chain Reaction  *Polymorphism,
       Restriction Fragment Length  Repetitive Sequences, Nucleic Acid  Species
       Specificity  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

