       Document 0377
 DOCN  M9620377
 TI    Detection of feline immunodeficiency virus in semen from seropositive
       domestic cats (Felis catus).
 DT    9602
 AU    Jordan HL; Howard J; Tompkins WA; Kennedy-Stoskopf S; Department of
       Microbiology, Pathology, and Parasitology, College; of Veterinary
       Medicine, North Carolina State University, Raleigh,; 27606, USA.
 SO    J Virol. 1995 Nov;69(11):7328-33. Unique Identifier : AIDSLINE
       MED/96013847
 AB    Electroejaculates from experimentally infected domestic cats were
       evaluated for the presence of feline immunodeficiency virus (FIV). Virus
       was isolated from cell-free seminal plasma and seminal cells by
       cocultivation with a feline interleukin-2-dependent CD4+ T-cell line, in
       which productive infection was demonstrated by syncytium formation and
       FIV gag p26 antigen secretion. In addition, an 868-bp segment of the FIV
       gag provirus gene was identified in cocultured cells by PCR and Southern
       analysis. A 582-bp fragment of the FIV gag provirus genome was detected
       by nested PCR and Southern analysis in nonfractionated seminal cells and
       in sperm purified by a swim-up procedure. This is the first report
       describing the detection of replication-competent FIV in cell-free and
       cell-associated forms in domestic cat semen.
 DE    Animal  Animals, Domestic  Blotting, Southern  Cats  Cell Line
       Coculture  CD4-Positive T-Lymphocytes  DNA, Viral/ANALYSIS  Genes, gag
       Giant Cells  Immunodeficiency Virus, Feline/*ISOLATION & PURIF  Male
       Polymerase Chain Reaction  Proviruses/ISOLATION & PURIF
       Semen/CYTOLOGY/*VIROLOGY  Simian Acquired Immunodeficiency
       Syndrome/*PHYSIOPATHOLOGY/  *VIROLOGY  Spermatozoa/*VIROLOGY  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, Non-P.H.S.  Support, U.S. Gov't,
       P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

