       Document 0362
 DOCN  M9620362
 TI    Correlation between AIDS-related Kaposi sarcoma histological grade and
       in vitro behavior: reduced exogenous growth factor requirements for
       isolates from high grade lesions.
 DT    9602
 AU    Bailer RT; Lazo A; Ng-Bautista CL; Hout BL; Ness GM; Hegtvedt AK; Lantry
       LE; Blakeslee JR; Stephens RE; Brierley GP; et al; Department of
       Veterinary Pathobiology, Ohio State University,; Columbus, USA.
 SO    Lymphology. 1995 Sep;28(3):126-37. Unique Identifier : AIDSLINE
       MED/96038359
 AB    Kaposi sarcoma, the most common AIDS-associated malignancy, affects
       10-30% of all AIDS patients. To date, research into the biological
       characteristics of AIDS-related Kaposi sarcoma (AIDS-KS) derived cell
       lines has been based on cultures established from skin explants or
       pleural effusions/peritoneal fluids. We have established several AIDS-KS
       lines from biopsy confirmed oral mucosal and epidermal AIDS-KS lesions
       and have found a correlation between AIDS-KS lesional grade and in vitro
       cellular growth characteristics. In comparison to epidermal AIDS-KS
       lesions, mucosal AIDS-KS lesions frequently possessed both a more
       advanced histologic grade and demonstrated a greater capacity to
       proliferate in minimal medium. We report the ability of AIDS-KS isolates
       from high grade lesions to sustain proliferation (greater than 60
       population doubling levels) in medium not supplemented with endothelial
       cell growth supplement and/or cytokine rich conditioned medium. These
       findings indicate that AIDS-KS cells isolated from high grade lesions
       have reduced requirements for exogenously provided growth supplements,
       and suggest that increased autologous cytokine production accompanies
       AIDS-KS lesional progression.
 DE    Acquired Immunodeficiency Syndrome/*COMPLICATIONS  Cell Cycle  Cell Line
       Culture Media  Cytokines/BIOSYNTHESIS  Human  Male  Mouth
       Neoplasms/*PATHOLOGY  Sarcoma, Kaposi's/ETIOLOGY/*PATHOLOGY  Skin
       Neoplasms/*PATHOLOGY  Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

