       Document 0359
 DOCN  M9620359
 TI    Establishment and characterization of a novel cell line, TK-6, derived
       from T cell blast crisis of chronic myelogenous leukemia, with the
       secretion of parathyroid hormone-related protein.
 DT    9602
 AU    Watanabe T; Kataoka T; Mizuta S; Kobayashi M; Uchida T; Imai K; Wada H;
       Kinoshita T; Murate T; Mizutani S; et al; First Department of Internal
       Medicine, Nagoya University School; of Medicine, Japan.
 SO    Leukemia. 1995 Nov;9(11):1926-34. Unique Identifier : AIDSLINE
       MED/96071146
 AB    We established a novel T cell line, designated TK-6, from a patient with
       T cell lineage blast crisis of chronic myelogenous leukemia (CML)
       complicated by hypercalcemia. A surface marker study showed T cell
       phenotype, cluster designation (CD)4, CD5 and CD7. Light and electron
       microscopic examination revealed myeloperoxidase (MPO)-negative,
       however, ultrastructural examination under certain specific conditions
       demonstrated that some cells were MPO-positive. The TK-6 cell karyotype
       carried a t(9;22)(q34;q11) and additional chromosome aberrations,
       including a deletion of the long arm of chromosome 6 and the abnormality
       of chromosome 7. Southern blot analysis showed rearrangement of the T
       cell receptor beta-chain (TCR beta) gene and the major breakpoint
       cluster region (bcr) gene. Northern blot analysis detected the
       expression of the parathyroid hormone-related protein (PTHrP) gene,
       however, the proviral genome of human T cell leukemia virus type I
       (HTLV-I) was negative. This cell line will provide a valuable resource
       for the analysis of the relationship between T cell lineage crisis and
       myeloid differentiation and for the analysis of humoral hypercalcemia of
       malignancy (HHM) or leukemia.
 DE    Adult  Base Sequence  Blast Crisis/*PATHOLOGY  Calcium/METABOLISM  Case
       Report  Cell Nucleus/ULTRASTRUCTURE  Chromosome Abnormalities/PATHOLOGY
       Chromosome Banding  Cytokines/METABOLISM  DNA Primers/CHEMISTRY  DNA,
       Viral/ANALYSIS  Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
       Gene Rearrangement, B-Lymphocyte, Heavy Chain  Genes, Immunoglobulin
       Human  HTLV-I/GENETICS  Immunophenotyping  Karyotyping  Leukemia,
       Myeloid, Chronic/*PATHOLOGY  Leukemia, Myeloid,
       Philadelphia-Positive/*PATHOLOGY  Male  Molecular Sequence Data
       Neoplasm Proteins/SECRETION  Proteins/*SECRETION  Support, Non-U.S.
       Gov't  T-Lymphocytes/*CYTOLOGY  Tumor Cells, Cultured/*CYTOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

