       Document 0357
 DOCN  M9620357
 TI    Glycosylation-dependent peptide antigenic determinants of env gp46
       HTLV-1.
 DT    9602
 AU    Kaumaya PT; Conrad SF; DiGeorge AM; Lairmore MD; Department of
       Obstetrics & Gynecology, College of Medicine, Ohio; State University,
       Columbus 43210, USA.
 SO    Leukemia. 1995 Oct;9 Suppl 1:S133-8. Unique Identifier : AIDSLINE
       MED/96022238
 AB    Human T-lymphotropic virus type 1 (HTLV-I) is a retrovirus associated
       with adult T-cell leukemia/lymphoma, and the virus infection constitutes
       a growing public health problem. In a continuing effort to engineer
       conformationally dependent HTLV-I epitopes that elicit a protective
       immune response, we have examined the role and functional importance of
       carbohydrate moieties in specific immune recognition and antibody
       responses. There have been several reports of the importance of N-linked
       virus glycosylation in the formation of neutralizing antibodies.
       Residues 230-257 is predicted to encode two beta-turn/loop regions at
       240-244 (LYGPN), 248-257 (VPSSSSTPL) and a glycosylation site at N-244
       (NVS). We have successfully engineered and synthesized the 233-253
       sequence of gp46 of HTLV-1 with and without GlcNAC at Asn244. Circular
       dichroism spectroscopy and proton NMR showed the presence of beta-turn
       conformation in both peptide constructs. Chimeras of the glycosylated
       and non-glycosylated epitope with promiscuous T-cell epitope were
       synthesized and shown to elicit high titered antibodies in rabbits
       specific for the immunogen (SC1MVF and SC2MVF) and the B cell epitope
       233-253. Additionally, antibodies to the glycosylated form of the
       peptide recognized the HTLV-I envelope precursor in radioimmunoassay
       precipitation assay and react with HTLV-I whole virus preparations in
       ELISA.
 DE    Adult  Amino Acid Sequence  Animal  Antibodies  Antibody Formation
       Enzyme-Linked Immunosorbent Assay  Epitopes/*ANALYSIS/CHEMISTRY  Gene
       Products, env/*BIOSYNTHESIS/GENETICS/*IMMUNOLOGY  Genes, env
       Glycopeptides/CHEMISTRY/IMMUNOLOGY/ISOLATION & PURIF  Glycosylation
       Human  HTLV-I/*GENETICS/*IMMUNOLOGY/ISOLATION & PURIF  Leukemia,
       T-Cell/*IMMUNOLOGY  Molecular Sequence Data  Nuclear Magnetic Resonance
       Peptide Fragments/CHEMISTRY/CHEMICAL SYNTHESIS/ISOLATION & PURIF
       Rabbits/IMMUNOLOGY  Retroviridae Proteins,
       Oncogenic/*BIOSYNTHESIS/GENETICS/  *IMMUNOLOGY  Support, U.S. Gov't,
       P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

