       Document 0234
 DOCN  M9620234
 TI    Transcription factor TFIID is a direct functional target of the
       adenovirus E1A transcription-repression domain.
 DT    9602
 AU    Song CZ; Loewenstein PM; Toth K; Green M; Institute for Molecular
       Virology, St. Louis University School of; Medicine, MO 63110, USA.
 SO    Proc Natl Acad Sci U S A. 1995 Oct 24;92(22):10330-3. Unique Identifier
       : AIDSLINE MED/96036078
 AB    The 243-amino acid adenovirus E1A oncoprotein both positively and
       negatively modulates the expression of cellular genes involved in the
       regulation of cell growth. The E1A transcription repression function
       appears to be linked with its ability to induce cellular DNA synthesis,
       cell proliferation, and cell transformation, as well as to inhibit cell
       differentiation. The mechanism by which E1A represses the transcription
       of various promoters has proven enigmatic. Here we provide several lines
       of evidence that the TATA-box binding protein (TBP) component of
       transcription factor TFIID is a cellular target of the E1A repression
       function encoded within the E1A N-terminal 80 amino acids. (i) The E1A
       N-terminal 80 amino acids [E1A-(1-80)protein] efficiently represses
       basal transcription from TATA-containing core promoters in vitro. (ii)
       TBP reverses completely E1A repression in vitro. (iii) TBP restores
       transcriptional activity to E1A-(1-80) protein affinity-depleted nuclear
       extracts. (iv) The N-terminal repression domain of E1A interacts
       directly and specifically with TBP in vitro. These results may help
       explain how E1A represses a set of genes that lack common upstream
       promoter elements.
 DE    Adenovirus E1A Proteins/*METABOLISM  Cell Differentiation  Cell Division
       Cell Nucleus/METABOLISM  Cell Transformation, Viral  Chromatography,
       Affinity  DNA Primers  DNA Replication  DNA-Binding Proteins/METABOLISM
       Hela Cells  Human  *HIV Long Terminal Repeat  Recombinant
       Proteins/METABOLISM  Support, U.S. Gov't, P.H.S.  Suppression, Genetic
       Templates  Transcription Factors/ISOLATION & PURIF/*METABOLISM
       *Transcription, Genetic  TATA Box  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

