       Document 0211
 DOCN  M9620211
 TI    Feeding the mosquito Aedes aegypti with TMOF and its analogs; effect on
       trypsin biosynthesis and egg development.
 DT    9602
 AU    Borovsky D; Mahmood F; University of Florida, IFAS, Florida Medical
       Entomology; Laboratory, Vero Beach 32962, USA.
 SO    Regul Pept. 1995 Jun 27;57(3):273-81. Unique Identifier : AIDSLINE
       MED/96070307
 AB    Female Aedes aegypti that were given a blood meal by enema deposited
       yolk in their oocytes and synthesized trypsinlike enzymes in their
       midgut. When females were given an enema of Aea-TMOF (Trypsin Modulating
       Oostatic Factor) (NH2-YDPAPPPPPP-COOH) and blood both egg development
       and trypsin biosynthesis were inhibited. Similar results were observed
       if TMOF was mixed with the blood meal and fed to female mosquitoes
       through a membrane. Renin inhibitor (NH2-PHPFHFFVYK-COOH) or poly
       proline given by enema with the blood meal did not affect egg
       development or trypsin biosynthesis. Feeding of TMOF analogs P1
       (NH2-YDPAP-COOH) or P4 (NH2-YDPAPPPP-COOH) inhibited trypsin
       biosynthesis in the midgut. Injecting or giving an enema of an amidated
       peptide (NH2-WRPGPPPPPP-CONH2) of HIV-2 X-ORF protein also inhibited egg
       development and trypsin biosynthesis in the mosquito gut. When [3H]TMOF
       was purified by high performance liquid chromatography (HPLC) and fed
       with the blood meal through a membrane to female mosquitoes, [3H]TMOF
       outside the gut increased linearly for the first 24 h and 28% of the
       hormone was found outside the gut at 72 h. These results suggest that
       TMOF and its active analogs traverse the gut epithelial cells into the
       hemolymph, bind TMOF gut receptor(s) and modulate trypsin biosynthesis.
 DE    Aedes/*DRUG EFFECTS/EMBRYOLOGY  Amino Acid Sequence  Animal  Biological
       Transport  Blood/PHYSIOLOGY  Enema  Female  Hemolymph/METABOLISM  Insect
       Hormones/*PHARMACOLOGY/PHARMACOKINETICS  Intestinal Mucosa/CYTOLOGY/DRUG
       EFFECTS/METABOLISM  Molecular Sequence Data
       Oligopeptides/*PHARMACOLOGY/PHARMACOKINETICS  Ovum/*DRUG EFFECTS
       Support, Non-U.S. Gov't  Support, U.S. Gov't, Non-P.H.S.
       Trypsin/*BIOSYNTHESIS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

