       Document 0198
 DOCN  M9620198
 TI    Morphological changes in lymph nodes and expression of VCAM1 and
       cytokines at the late stages of SIV-induced disease in rhesus monkeys.
 DT    9602
 AU    Persidsky Y; Steffan AM; Gendrault JL; Royer C; Beyer C; Muchmore E;
       Kirn A; Aubertin AM; Unite INSERM 74, France.
 SO    Res Virol. 1995 May-Jun;146(3):185-200. Unique Identifier : AIDSLINE
       MED/96075769
 AB    Four patterns of structural alterations were found in lymph nodes (LNs)
       from rhesus monkeys 17 to 34 months after infection with simian
       immunodeficiency virus (SIV-mac251). SIV p27gag antigen and viral
       particles were localized either between the processes of follicular
       dendritic cells (FDCs) or in the cytoplasm of macrophages. In
       hyperplastic follicles, enlarged germinal centres contained numerous
       Ki67+ proliferating centroblasts which were rather rare in light zones
       occupied by the CD23+ FDC network. Involuted follicles contained a small
       number of Ki67+ centroblasts and the CD23 labelling was limited to a
       very small apical zone. A correlation was found between the
       morphological characteristics of the follicles (hyperplasia-involution)
       and the level of expression of the vascular cell adhesion molecule 1
       (VCAM1) on FDCs. A gradient in VCAM1 intensity with no expression in the
       subcapsular-intermediary sinuses, low membrane labelling in the mantle
       and strong expression in the FDC network was observed. IL1 alpha+ and
       IL6+ (interleukin) cells (lymphocytes and macrophages) were detected in
       the mantle, the interfollicular area and the medulla of LNs. Expression
       of the tumour necrosis factor alpha and ultrastructural markers of
       interferon alpha production were found in a few FDC and macrophages. Our
       findings indicate a close relationship between the morphofunctional
       properties of FDC and the LN structure in SIV infection.
 DE    Animal  Antigens, Viral/BIOSYNTHESIS  Cell Line  Cytokines/*BIOSYNTHESIS
       Gene Products, gag/METABOLISM  Lymph Nodes/IMMUNOLOGY/*ULTRASTRUCTURE
       Macaca mulatta  Receptors, IgE/BIOSYNTHESIS  Simian Acquired
       Immunodeficiency Syndrome/IMMUNOLOGY/*PATHOLOGY  *SIV/ULTRASTRUCTURE
       Vascular Cell Adhesion Molecule-1/*BIOSYNTHESIS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

