       Document 0124
 DOCN  M9620124
 TI    Genetic and antigenic characterization of caev (caprine
       arthritis-encephalitis virus) recombinant transmembrane protein.
 DT    9602
 AU    Rosati S; Pittau M; Tolari F; Erre G; Kwang J; Dipartimento di
       Produzioni Animali, Epidemiologia ed Ecologia,; Universita di Torino,
       Italy.
 SO    Vet Microbiol. 1995 Aug;45(4):363-70. Unique Identifier : AIDSLINE
       MED/96021590
 AB    The env gene fragment of an Italian strain of Caprine Arthritis
       Encephalitis virus (CAEV) coding for the hydrophilic region of
       transmembrane protein was amplified, cloned and expressed in prokaryotic
       system as fusion protein with glutathione-S-transferase. Sequence
       analysis revealed 63 to 66% amino acid homology, when compared with
       three ovine lentiviruses and 83% when compared with one caprine
       lentivirus. The recombinant transmembrane protein was efficiently
       expressed, purified under denaturing conditions and used as antigen in
       western blotting and ELISA. Sera from clinically diseased goats strongly
       reacted in western blotting and naturally infected animals seroconverted
       between 20 and 33 weeks of age. An indirect ELISA performed with this
       antigen showed improved sensitivity in comparison with agar gel
       immunodiffusion test. Our results confirm that transmembrane protein is
       an important immunological marker in CAEV infection and its use as
       antigen may enhance the validity of serological diagnosis of Caprine
       Arthritis Encephalitis.
 DE    Amino Acid Sequence  Animal  Arthritis-Encephalitis Virus,
       Caprine/*GENETICS  Base Sequence  Blotting, Western  Cloning, Molecular
       Comparative Study  Enzyme-Linked Immunosorbent Assay  Gene Expression
       Gene Products, env/BIOSYNTHESIS/*CHEMISTRY/*GENETICS  *Genes, env
       Glutathione Transferases  Goats  Immunodiffusion  Lentivirus/GENETICS
       Molecular Sequence Data  Recombinant Fusion
       Proteins/BIOSYNTHESIS/CHEMISTRY/IMMUNOLOGY  Reproducibility of Results
       Sensitivity and Specificity  Sequence Homology, Amino Acid  Sequence
       Homology, Nucleic Acid  Sheep  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

