       Document 0118
 DOCN  M9620118
 TI    Recovery of virtually full-length HIV-1 provirus of diverse subtypes
       from primary virus cultures using the polymerase chain reaction.
 DT    9602
 AU    Salminen MO; Koch C; Sanders-Buell E; Ehrenberg PK; Michael NL; Carr JK;
       Burke DS; McCutchan FE; Henry M. Jackson Foundation for the Advancement
       of Military; Medicine, Rockville, Maryland 20850, USA.
 SO    Virology. 1995 Oct 20;213(1):80-6. Unique Identifier : AIDSLINE
       GENBANK/M19921
 AB    In the course of the global pandemic, the human immunodeficiency virus
       type-1 (HIV-1) has established at least eight distinct genotypes in the
       main (M), or prevalent, group of isolates, a variety of rare outlier
       forms, and intergenotypic recombinants of group M viruses. This
       genotypic diversity has been documented, for the most part, by
       sequencing of subgenomic segments of the provirus. Using DNA from virus
       cultures on peripheral blood mononuclear cells (PBMC) and recent
       improvements of the PCR technique, we have amplified virtually
       full-length HIV-1 genomes from genetic subtypes A through G of group M
       viruses and molecularly cloned several of them. Resequencing of the
       complete genome of a prototype strain after long PCR amplification and
       cloning has established a PCR error rate of 0.14%. We also report the
       first complete PCR-derived sequence of a U.S. clinical isolate of
       genotype B expanded only in primary PBMC; this provirus harbors a
       uniquely truncated V3 loop.
 DE    Amino Acid Sequence  Base Sequence  Cloning, Molecular  DNA
       Primers/CHEMISTRY  DNA, Viral/*ANALYSIS  Electrophoresis, Agar Gel
       *Genes, Viral  Genotype  Human  HIV-1/*GENETICS/PHYSIOLOGY  Leukocytes,
       Mononuclear/VIROLOGY  Molecular Sequence Data  Polymerase Chain
       Reaction/*METHODS  Proviruses/*GENETICS/PHYSIOLOGY  Support, Non-U.S.
       Gov't  Virus Cultivation  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

