       Document 0117
 DOCN  M9620117
 TI    Genotypic characteristics of HTLV-II isolates from Amerindian and
       non-Indian populations.
 DT    9602
 AU    Pardi D; Hjelle B; Folks TM; Lal RB; Retrovirus Diseases Branch, Centers
       for Disease Control and; Prevention, Atlanta, Georgia 30333, USA.
 SO    Virus Genes. 1995;10(1):27-35. Unique Identifier : AIDSLINE
       GENBANK/L11456
 AB    The Amerindian human T-cell lymphotropic virus type II isolate
       HTLV-IIG12 has been demonstrated to be an HTLV-IIb with several unique
       features, including several restriction enzyme site changes, a
       distinctive pre-gag region, a stop codon within the pol gene, and an
       extended Tax protein. In this study, HTLV-II isolates from Amerindian
       and non-Indian populations were characterized by restriction enzyme site
       analysis to determine the prevalent HTLV-II subtype. In addition, DNA
       amplification by the polymerase chain reaction and Southern blot
       analyses were used to probe for the HTLV-IIG12 pre-gag region. Our
       results showed that of 13 Guaymi Indian isolates subtyped, all were
       HTLV-IIb, and that approximately one third of 17 isolates had the unique
       pre-gag region. While other HTLV-II-infected groups contained both
       HTLV-IIa and HTLV-IIb isolates, none of these isolates showed evidence
       of the distinctive HTLV-IIG12 pre-gag region. Lastly, DNA sequence
       analysis was used to determine the prevalence of the stop codon within
       the pol gene open reading frame. These analyses revealed that the
       occurrence of a stop codon within this sequence appeared to be
       characteristic of most HTLV-IIb subtypes. These results further our
       understanding of the genetic variations and evolution of the HTLV-II
       viruses within the endemically infected Amerindian populations, as well
       as U.S. intravenous drug users and other non-Indian populations.
 DE    Base Sequence  Genes, env  Genes, gag  Genes, pol  Genotype  Human
       HTLV-II/*GENETICS/ISOLATION & PURIF  HTLV-II Infections/BLOOD/*VIROLOGY
       *Indians, Central American  *Indians, North American  Molecular Sequence
       Data  Protein Precursors/GENETICS  Sequence Homology, Nucleic Acid
       Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

