       Document 0900
 DOCN  M9620900
 TI    Cytotoxic T lymphocytes specific for the synthetic VEINCTR peptide, a
       sequence found within the Fas molecule and env gp120 in the blood of
       HIV-1 seropositive individuals.
 DT    9602
 AU    Moukrim Z; Achour A; Laboratoire de Physiologie Cellulaire, Universite
       Pierre et; Marie Curie, Paris, France.
 SO    Cell Mol Biol (Noisy-le-grand). 1995 May;41(3):439-44. Unique Identifier
       : AIDSLINE MED/96059406
 AB    We have previously identified a VEINCTR peptide common to both the
       Fasmolecule and HIV-1 gp120. Here we report the characterization in
       PBMCs from HIV-1-infected individuals of a CD8+ class I restricted CTL
       activity directed towards this peptide. The peptide is highly conserved
       in various HIV-1 strains, being located at amino acid 287-293 (VEINCTR),
       within an epitope known as cell T epitope on the env protein of human
       immunodeficiency virus type-1. Cell cultures were obtained by polyclonal
       activation using autologous blast cells and CTL lines generated from
       frozen peripheral blood lymphocytes of HIV-1 seropositive donors by
       stimulation with the peptide and recombinant interleukin-2. The
       env-specific CTL turned out to kill autologous target cells infected
       with a recombinant vaccinia virus containing the env gene of HIV-1 or
       pulsed with peptide. Specificity was determined using shorter peptides.
       The CTL activity was directed against autologous target cells presenting
       the heptapeptide which is site located in the Fas molecule, known to be
       functionally involved in T-cell apoptosis.
 DE    Acquired Immunodeficiency Syndrome/*IMMUNOLOGY  Amino Acid Sequence
       Antigens, CD95/GENETICS/*IMMUNOLOGY  Apoptosis
       B-Lymphocytes/IMMUNOLOGY/VIROLOGY  Cell Line, Transformed  Cytotoxicity
       Tests, Immunologic  Epitopes/*IMMUNOLOGY  Human  HIV Envelope Protein
       gp120/GENETICS/*IMMUNOLOGY  HLA-A2 Antigen/IMMUNOLOGY  Leukocytes,
       Mononuclear/IMMUNOLOGY  Lymphocyte Transformation  Molecular Sequence
       Data  Oligopeptides/*IMMUNOLOGY  Peptide Fragments/IMMUNOLOGY  Support,
       Non-U.S. Gov't  T-Lymphocytes, Cytotoxic/*IMMUNOLOGY  Vaccinia
       Virus/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

