       Document 0179
 DOCN  M9620179
 TI    Both immunization with protein and recombinant vaccinia virus can
       stimulate CTL specific for the E7 protein of human papilloma virus 16 in
       H-2d mice.
 DT    9602
 AU    Zhu X; Tommasino M; Vousden K; Sadovnikava E; Rappuoli R; Crawford L;
       Kast M; Melief CJ; Beverley PC; Stauss HJ; MCR Tuberculosis and Related
       Infections Unit, Royal Postgraduate; Medical School, Hammersmith
       Hospital, London, UK.
 SO    Scand J Immunol. 1995 Nov;42(5):557-63. Unique Identifier : AIDSLINE
       MED/96062122
 AB    The transforming protein E7 of human papilloma virus type 16 can
       stimulate cytotoxic T lymphocytes (CTL) which can protect experimental
       animals against growth of E7 expressing tumour cells. In this study we
       compared CTL responses in mice immunized with either E7 protein in MF59
       adjuvant or with recombinant vaccinia virus expressing E7 (Vac-E7). We
       have chosen H-2d mice because no E7-specific CTL responses have been
       described in this MHC haplotype. Immunization of these mice with Vac-E7
       generated CTL which lysed target cells infected with Vac-E7 or
       transfected with the E7 gene. CTL from mice immunized with E7 protein in
       MF59 adjuvant showed specificity for the same target cells. Antibody
       blocking experiments revealed that both immunization with Vac-E7 and E7
       protein stimulated CD8+ effector CTL. The find specificity of CTL
       induced by the two immunization protocols was similar. A major CTL
       epitope was mapped to the carboxyl terminal amino acids 48-98 of the E7
       protein. Peptide isolation from E7 expressing cells followed by HPLC
       separation indicated that CTL induced by immunization with protein and
       Vac-E7 recognized the same HPLC purified peptide fractions. Together,
       the study suggests that vaccines based on protein can activate CTL with
       similar fine specificity to CTL induced by vaccines based on recombinant
       vaccinia virus.
 DE    Adjuvants, Immunologic  Amino Acid Sequence  Animal  Cytotoxicity,
       Immunologic  CD8-Positive T-Lymphocytes/*IMMUNOLOGY  Epitopes,
       T-Lymphocyte/IMMUNOLOGY  Mice  Mice, Inbred BALB C  Mice, Inbred DBA
       Molecular Sequence Data  Oncogene Proteins, Viral/*IMMUNOLOGY
       Peptides/CHEMISTRY/IMMUNOLOGY  T-Lymphocytes, Cytotoxic/*IMMUNOLOGY
       Vaccines, Synthetic/*IMMUNOLOGY  Vaccinia Virus  Viral
       Vaccines/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

