       Document 0463
 DOCN  M9620463
 TI    Function of human immunodeficiency virus type 1 Vpu protein in various
       cell types.
 DT    9602
 AU    Sakai H; Tokunaga K; Kawamura M; Adachi A; Institute for Virus Research,
       Kyoto University, Japan.
 SO    J Gen Virol. 1995 Nov;76 ( Pt 11):2717-22. Unique Identifier : AIDSLINE
       MED/96068804
 AB    We evaluated the function of human immunodeficiency virus type 1 vpu
       gene in various cell lines. We established a highly sensitive system
       consisting of chloramphenicol acetyltransferase and reverse
       transcriptase assays and used it to monitor the effects of mutation of
       the vpu gene. In some cell lines, Vpu protein was not required at the
       early phase of viral replication but was important for efficient virion
       production. In these cells, the Vpu protein functioned effectively
       irrespective of the presence of intact env gene products. Likewise, CD4
       gene expression had no effects on Vpu function. In the other cell lines
       tested, Vpu protein was not important for virion release, and the vpu
       mutant clone generated a normal level of progeny virions upon
       transfection.
 DE    Animal  Cell Line  CD4-Positive T-Lymphocytes/CYTOLOGY/VIROLOGY  Gene
       Products, vpu/GENETICS/*PHYSIOLOGY  *Genes, vpu  Hela Cells  Human
       HIV-1/GENETICS/*PHYSIOLOGY  Mice  Mutation  Support, Non-U.S. Gov't
       Virus Replication  3T3 Cells  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

