       Document 0480
 DOCN  M9620480
 TI    [Prior enrichment of HIV-DNA with probe-DNA particles for an efficient
       PCR diagnosis]
 DT    9602
 AU    Fan K; Tano H; Kitajima M; Tamatsukuri S; Furuichi Y; Hayashi T; Imai M;
       Japan Synthetic Rubber Co., Ltd. Tsukuba Research Laboratory.
 SO    Kansenshogaku Zasshi. 1995 Sep;69(9):957-62. Unique Identifier :
       AIDSLINE MED/96056823
 AB    PCR mediated detection of HIV-DNA has been widely used. However,
       compared with traditional immunological diagnoses, the extraction of DNA
       is a laborious and time consuming step. We have developed a procedure
       for the efficient isolation and concentration of HIV-DNA from cell
       lysates. We report here a novel method by one can recover HIV-DNA in a
       small volume (approximately 50 microliters) of solution from a large
       volume of crude cell lysate which contains as few as several copies. The
       method uses the specific hybridization of HIV-DNA to HIV probe-DNA
       particles. This prior enrichment augmented the sensitivity in the
       detection of HIV-DNA by PCR, and allows us to make a diagnosis even if
       the specimen contained an extremely low copy number of HIV-DNA molecules
       in a large volume, which would have otherwise resulted in false-negative
       data with the conventional extraction method. The method also enables
       the examination of 100 individual blood specimens in a combined form.
       Thus, the application of the present enrichment procedure with HIV
       probe-DNA particles should reduce the labor and cost of HIV diagnosis,
       since the HIV positive samples represent a very minor group of people
       among specimens subjected to clinical laboratory tests, and
       particularly, among blood samples voluntarily donated to be used for
       transfusions.
 DE    Base Sequence  Centrifugation  DNA Primers  *DNA Probes  DNA,
       Viral/*ANALYSIS/ISOLATION & PURIF  English Abstract  Human
       HIV/*GENETICS  HIV Infections/DIAGNOSIS  Molecular Sequence Data
       Polymerase Chain Reaction  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

