       Document 0886
 DOCN  M9620886
 TI    Short-term in vitro culture and molecular analysis of the
       microsporidian, Enterocytozoon bieneusi.
 DT    9602
 AU    Visvesvara G; Leitch GJ; Pieniazek NJ; Da Silva AJ; Wallace S; Slemenda
       SB; Weber R; Schwartz DA; Gorelkin L; Wilcox CM; et al; Division of
       Parasitic Diseases, National Center for Infectious; Diseases, Centers
       for Disease Control and Prevention, Public; Health Service, U.S.
       Department of Health and Human Services,; Atlanta, Georgia 30341-3724,
       USA.
 SO    J Eukaryot Microbiol. 1995 Sep-Oct;42(5):506-10. Unique Identifier :
       AIDSLINE MED/96010583
 AB    The microsporidium, Enterocytozoon bieneusi, causes a severe,
       debilitating, chronic diarrhea in patients with the acquired
       immunodeficiency syndrome. Specific diagnosis of intestinal
       microsporidiosis, especially due to Enterocytozoon, is difficult and
       there is no known therapy that can completely eradicate this parasite.
       Preliminary studies indicate that a short term (about 6 months) in vitro
       culture of this parasite yielding low numbers of spores, may be
       established by inoculating human lung fibroblasts and/or monkey kidney
       cell cultures with duodenal aspirates and or biopsy from infected
       patients. The cultures may subsequently be used for the isolation and
       molecular analysis of parasite DNA.
 DE    Adult  Animal  AIDS-Related Opportunistic
       Infections/DIAGNOSIS/PARASITOLOGY  Base Sequence  Cells, Cultured
       Cercopithecus aethiops  DNA, Protozoan/GENETICS  DNA, Ribosomal/GENETICS
       Fibroblasts  Human  Lung  Male  Microspora
       Infections/DIAGNOSIS/*PARASITOLOGY  Microsporida/*GROWTH &
       DEVELOPMENT/*GENETICS/ISOLATION & PURIF/  ULTRASTRUCTURE  Middle Age
       Molecular Sequence Data  Parasitology/METHODS  RNA, Ribosomal/*GENETICS
       Sequence Analysis, DNA  Support, U.S. Gov't, P.H.S.  Vero Cells  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

