       Document 0994
 DOCN  M9620994
 TI    Detection of Epstein-Barr virus in cutaneous and lymph nodal anaplastic
       large cell lymphomas (Ki-1+).
 DT    9602
 AU    Peris K; Niedermeyer H; Chimenti S; Radaskiewicz T; Kerl H; Hoefler H;
       Department of Dermatology, Universities of L'Aquila, Italy.
 SO    Br J Dermatol. 1995 Oct;133(4):542-6. Unique Identifier : AIDSLINE
       MED/96096852
 AB    Epstein-Barr virus (EBV) is a gamma DNA herpes virus which is thought to
       play a part in the pathogenesis of some non-Hodgkin's lymphomas in
       individuals with or without immunodeficiency. We investigated 16 lymph
       nodal and 12 cutaneous anaplastic large cell lymphomas (ALCLs) (Ki-1+),
       all of which were in patients without immunodeficiency, for the presence
       of EBV genomes. The highly sensitive polymerase chain reaction (PCR)
       technique was employed for detection of viral DNA in extracts from
       formalin-fixed, paraffin-embedded tissue sections. In addition, we
       performed radioactive and non-radioactive in situ hybridization (ISH)
       for localization of EBV at the single cell level. EBV-DNA was
       demonstrated by PCR in five cases of nodal ALCLs (31%). All cutaneous
       ALCLs were negative. EBV-encoded small nuclear RNAs (EBERs) could be
       identified by ISH in the tumour cells of one of the five
       EBV-DNA-positive patients. Our results further support the concept that
       EBV may be involved in the development of a proportion of nodal ALCLs,
       but not in cutaneous ALCLs.
 DE    DNA, Viral/ANALYSIS  Herpesvirus 4, Human/*ISOLATION & PURIF  Human  In
       Situ Hybridization  Lymphoma, Large-Cell, Ki-1/*VIROLOGY  Lymphoma,
       T-Cell, Cutaneous/*VIROLOGY  Polymerase Chain Reaction  RNA, Small
       Nuclear/ANALYSIS  RNA, Viral/ANALYSIS  Skin Neoplasms/*VIROLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

