       Document 0684
 DOCN  M9620684
 TI    Evaluation of T-lymphocyte subsets present in semen and peripheral blood
       of healthy donors: a report from the heterosexual transmission study.
 DT    9602
 AU    Denny TN; Scolpino A; Garcia A; Polyak A; Weiss SN; Skurnick JH;
       Passannante MR; Colon J; Departments of Pediatrics and Pathology, New
       Jersey Medical; School, Newark, USA.
 SO    Cytometry. 1995 Aug 1;20(4):349-55. Unique Identifier : AIDSLINE
       MED/96101991
 AB    The purpose of this study was to accurately determine the T-lymphocyte
       subsets found in semen from healthy volunteers, to evaluate the impact
       of repeated ejaculation on the frequency or type of immune cells present
       in semen, and to compare subset analysis in semen to that in the
       peripheral blood. To accomplish this, a flow cytometric method was
       developed to identify and count immunophenotypically distinct cells
       present in semen. Fresh semen samples and peripheral blood were
       collected over three consecutive days from nine healthy donors. Donors
       had normal ejaculate volume, sperm count, sperm motility, morphology,
       and leukocyte count. No significant intra-donor differences were seen in
       these parameters over time. No significant differences were observed in
       the percentage of CD3+ cells, CD4+ cells, CD8+ cells, and the CD4:CD8
       ratio in semen on consecutive days. However, within the CD4+ subset,
       when naive and memory CD4+ cells were measured, some day to day
       variability was suggested. No significant differences in CD3+, CD4+,
       CD8+, CD4/CD8 ratio, or naive and memory subsets were seen in the
       peripheral blood between sampling days. When semen was compared to
       peripheral blood some differences in immune subset values were observed,
       with an increase in the percentage of memory CD4+ cells in semen being
       the most striking. This finding may be relevant to HIV transmission,
       since others have shown that this cell may be preferentially infected
       with HIV and is the primary reservoir for virus in infected individuals.
 DE    Adult  Blood Cells  Comparative Study  CD4 Lymphocyte Count  CD4-CD8
       Ratio  *Ejaculation  Flow Cytometry/*METHODS  Human  HIV
       Infections/TRANSMISSION  HIV Seronegativity/*IMMUNOLOGY
       Immunophenotyping/*METHODS  *Lymphocyte Count  Male  Reference Values
       Semen/*CYTOLOGY/IMMUNOLOGY  Support, U.S. Gov't, P.H.S.  *T-Lymphocyte
       Subsets  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

