       Document 0406
 DOCN  M9620406
 TI    A single retroviral gag precursor signal peptide recognized by FBL-3
       tumor-specific cytotoxic T lymphocytes.
 DT    9602
 AU    Kondo T; Uenishi H; Shimizu T; Hirama T; Iwashiro M; Kuribayashi K;
       Tamamura H; Fujii N; Fujisawa R; Miyazawa M; et al; Department of
       Biophysics, Faculty of Science, Kyoto University,; Japan.
 SO    J Virol. 1995 Nov;69(11):6735-41. Unique Identifier : AIDSLINE
       GENBANK/D45226
 AB    Several dominant T-cell receptors of cytotoxic T-lymphocyte (CTL) clones
       specific for FBL-3 tumor antigen were clonally amplified in mixed
       lymphocyte tumor cell cultures derived from an individual immune mouse.
       Every CTL clone analyzed had a common specificity for a single epitope
       in the precursor to cell membrane-associated nonstructural gag-encoded
       protein, Pr75gag, which can be minimally identified by nine amino acid
       residues, SIVLCCLCL. This epitope is located within the hydrophobic
       signal sequence motif that mediates translocation of the protein into
       the endoplasmic reticulum. These novel observations suggest that
       expression of Pr75gag in FBL-3 tumor cells led to the amplification of
       CTLs which recognize the signal sequence of the nonstructural
       gag-encoded glycoprotein precursor.
 DE    Amino Acid Sequence  Animal  Base Sequence  Clone Cells  Cloning,
       Molecular  DNA Primers  Endoplasmic Reticulum/IMMUNOLOGY/METABOLISM
       Friend Virus/GENETICS/*IMMUNOLOGY  Gene Products,
       gag/BIOSYNTHESIS/*IMMUNOLOGY  Genes, gag  Leukemia, Erythroblastic,
       Acute/IMMUNOLOGY  Mice  Mice, Inbred Strains  Molecular Sequence Data
       Polymerase Chain Reaction  Protein Precursors/IMMUNOLOGY  Receptors,
       Antigen, T-Cell, alpha-beta/*BIOSYNTHESIS/*IMMUNOLOGY  Recombinant
       Proteins/BIOSYNTHESIS/IMMUNOLOGY  Signal Peptides/*IMMUNOLOGY  Support,
       Non-U.S. Gov't  T-Lymphocytes, Cytotoxic/*IMMUNOLOGY/*VIROLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

