       Document 0518
 DOCN  M9620518
 TI    Recombinant human growth-regulated oncogene-alpha induces T lymphocyte
       chemotaxis. A process regulated via IL-8 receptors by IFN-gamma,
       TNF-alpha, IL-4, IL-10, and IL-13.
 DT    9602
 AU    Jinquan T; Frydenberg J; Mukaida N; Bonde J; Larsen CG; Matsushima K;
       Thestrup-Pedersen K; Department of Dermatology, University of Aarhus,
       Denmark.
 SO    J Immunol. 1995 Dec 1;155(11):5359-68. Unique Identifier : AIDSLINE
       MED/96072814
 AB    The human cytokine growth-regulated oncogene (GRO)-alpha is a small
       glycoprotein secreted by monocytes, endothelial cells, glycoprotein
       secreted by monocytes, endothelial cells, fibroblasts, synovial cells,
       and some tumor cells such as melanoma cells. It is structurally related
       to IL-8 and can activate neutrophils, whereas it induces chemotaxis,
       exocytosis, and a respiratory burst in neutrophils. To date, its
       functions on T lymphocytes have not been well established. We report
       here that recombinant human (rh)GRO-alpha is a potent chemoattractant
       for freshly isolated T lymphocytes, but not for anti-CD3 mAb-activated T
       lymphocytes. It attracts CD4+ and CD8+ T lymphocyte subsets to an equal
       extent. The migrating T lymphocytes toward rhGRO-alpha are predominantly
       CD45RO+ memory CD4+ and CD8+ subsets. The chemotactic migration of T
       lymphocytes toward rhGRO-alpha is stimulated via the IL-8 receptors on
       the cells. This process can be augmented by IFN-gamma and TNF-alpha, and
       inhibited by IL-4, IL-10, and IL-13. In addition, we also document that
       on T lymphocytes there exist IL-8 receptors that can be up-regulated by
       IFN-gamma, TNF-alpha, and IL-2. Our results demonstrate that rhGRO-alpha
       gene encodes for an inflammatory mediator that stimulates the
       directional migration of T lymphocytes. It may thus be another important
       mediator in the diseases in which T lymphocytes form the major
       constituent of the cellular infiltration.
 DE    Antigens, CD/DRUG EFFECTS/IMMUNOLOGY/*METABOLISM  Antigens,
       CD3/IMMUNOLOGY  Base Sequence  Chemotactic
       Factors/GENETICS/*PHARMACOLOGY  Chemotaxis, Leukocyte/*DRUG EFFECTS
       CD4-Positive T-Lymphocytes/DRUG EFFECTS  CD8-Positive T-Lymphocytes/DRUG
       EFFECTS  Growth Substances/GENETICS/*PHARMACOLOGY  Human
       Immunophenotyping  Interferon Type II/METABOLISM
       Interleukins/*METABOLISM  Molecular Sequence Data  Receptors,
       Interleukin/DRUG EFFECTS/IMMUNOLOGY/*METABOLISM  Recombinant
       Proteins/GENETICS/PHARMACOLOGY  RNA, Messenger/ANALYSIS  Support,
       Non-U.S. Gov't  T-Lymphocyte Subsets/*DRUG EFFECTS  Tumor Necrosis
       Factor/METABOLISM  Up-Regulation (Physiology)/GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

