       Document 0354
 DOCN  M9620354
 TI    Inhibition of human immunodeficiency virus type 1 (HIV) gag gene
       expression by an antisense oligodeoxynucleotide phosphorothioate.
 DT    9602
 AU    Anazodo MI; Wainberg MA; Friesen AD; Wright JA; Manitoba Institute of
       Cell Biology, University of Manitoba,; Winnipeg, Canada.
 SO    Leukemia. 1995 Oct;9 Suppl 1:S86-8. Unique Identifier : AIDSLINE
       MED/96022227
 AB    We have employed a cos-like monkey kidney cell line (B4.14) transfected
       with plasmids pCMVgag-pol-rre-r (containing the HIV gag and pol genes)
       and pCMVrev (containing the HIV rev gene), as a model to investigate
       whether antisense constructs could interfere with specific HIV gene and
       protein expression. We utilized an antisense construct (GP12A) directed
       against a non-regulatory region of the HIV genome, to transfect cells
       that expressed the above-mentioned HIV genes. Our results show that
       GP12A was able to attenuate levels of relevant HIV mRNA and gag proteins
       in the absence of cytotoxic effects.
 DE    Animal  Antiviral Agents/*PHARMACOLOGY  Base Sequence  Cell Line
       Cercopithecus aethiops  Gene Expression/*DRUG EFFECTS  Gene Products,
       gag/*BIOSYNTHESIS  Genes, gag/*DRUG EFFECTS  Genes, pol  Genome, Viral
       Human  HIV-1/DRUG EFFECTS/*GENETICS  Kidney  Molecular Sequence Data
       Oligonucleotides, Antisense/*PHARMACOLOGY  Regulatory Sequences, Nucleic
       Acid  Thionucleotides  Transcription, Genetic/DRUG EFFECTS  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

