       Document 0392
 DOCN  M9620392
 TI    Isolation and characterization of a new simian T-cell leukemia virus
       type 1 from naturally infected celebes macaques (Macaca tonkeana):
       complete nucleotide sequence and phylogenetic relationship with the
       Australo-Melanesian human T-cell leukemia virus type 1.
 DT    9602
 AU    Ibrahim F; de The G; Gessain A; Unite d'Epidemiologie des Virus
       Oncogenes, Institut Pasteur,; France.
 SO    J Virol. 1995 Nov;69(11):6980-93. Unique Identifier : AIDSLINE
       GENBANK/Z46900
 AB    A study of simian T-cell leukemia virus type 1 (STLV-1) infection in a
       captive colony of 23 Macaca tonkeana macaques indicated that 17 animals
       had high human T-cell leukemia virus type 1 (HTLV-1) antibody titers.
       Genealogical analysis suggested mainly a mother-to-offspring
       transmission of this STLV-1. Three long-term T-cell lines, established
       from peripheral blood mononuclear cell cultures from three
       STLV-1-seropositive monkeys, produced HTLV-1 Gag and Env antigens and
       retroviral particles. The first complete nucleotide sequence of an
       STLV-1 (9,025 bp), obtained for one of these isolates, indicated an
       overall genetic organization similar to that of HTLV-1 but with a
       nucleotide variability for the structural genes ranging from 7.8 to
       13.1% compared with the HTLV-1 ATK and STLV-1 PTM3 Asian prototypes. The
       Tax and Rex regulatory proteins were well conserved, while the pX
       region, known to encode new proteins in HTLV-1 (open reading frames I
       and II), was more divergent than that in the ATK strain. Furthermore, a
       fragment of 522 bp of the gp21 env gene from uncultured peripheral blood
       mononuclear cell DNAs from five of the STLV-1-infected monkeys was
       sequenced. Phylogenetic trees constructed with the long terminal repeat
       and env (gp46 and gp21) regions demonstrated that this new STLV-1
       occupies a unique position within the Asian STLV-1 and HTLV-1 isolates,
       being, by most analyses, related more to the Australo-Melanesian HTLV-1
       topotype than to any other Asian STLV-1. These data raise new hypotheses
       on the possible interspecies viral transmission between monkeys carrying
       STLV-1 and early Australoid settlers, ancestors of the present day
       Australo-Melanesian inhabitants, during their migrations from the
       Southeast Asian land mass to the greater Australian continent.
 DE    Amino Acid Sequence  Animal  Antigens, Viral/ANALYSIS  Australia  Base
       Sequence  Binding Sites  Cloning, Molecular  Comparative Study  DNA
       Primers  Enhancer Elements (Genetics)  Female  Gene Products,
       rex/METABOLISM  Human  HTLV-BLV Infections/*VIROLOGY
       HTLV-I/CLASSIFICATION/*GENETICS  Macaca/GENETICS/*VIROLOGY  Male
       Melanesia  Molecular Sequence Data  Pedigree  *Phylogeny  Sequence
       Homology, Amino Acid  Sequence Homology, Nucleic Acid  Support, Non-U.S.
       Gov't  STLV/CLASSIFICATION/*GENETICS/*ISOLATION & PURIF  TATA Box
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

