       Document 1038
 DOCN  M9621038
 TI    Insulin-like growth factor 1 and insulin inhibit HIV type 1 replication
       in cultured cells.
 DT    9602
 AU    Germinario RJ; DeSantis T; Wainberg MA; Lady Davis Institute for Medical
       Research, Sir Mortimer B.; Davis-Jewish General Hospital, Montreal,
       Quebec, Canada.
 SO    AIDS Res Hum Retroviruses. 1995 May;11(5):555-61. Unique Identifier :
       AIDSLINE MED/96093890
 AB    Insulin-like growth factor 1 and insulin, considered primarily as
       metabolic and growth modulatory hormones, were found to inhibit the
       replication of HIV-1 in cultured cord blood mononuclear cells and
       chronically HIV-infected U937 cells. The effect of IGF-1 was seen at
       physiological concentrations or lower (1.7 x 10(-10) M) while that of
       insulin was observed at supraphysiological concentrations (8 x 10(-7)
       M). The EC50 for IGF-1 was found to be in the physiological range
       (2.5-4.5 x 10(-9) M) while that for insulin was considerably higher
       (1.1-3.3 x 10(-6) M). Insulin-like growth factor 1 and insulin at the
       concentrations employed exhibited no toxicity on the cells used in these
       studies. Furthermore, neither IGF-1 nor insulin exhibited any inhibitory
       activity on purified reverse transcriptase in vitro. Epidermal growth
       factor from 1.6 x 10(-10) to 1.6 x 10(-8) M demonstrated no inhibition
       of HIV-1 replication, while IGF-1 inhibited p24 antigen production 49
       and 42% at 1.3 x 10(-9) and 1.3 x 10(-8) M IGF-1, respectively. These
       results suggest that IGF-1 under certain conditions has significant
       inhibitory effects on HIV-1 replication at physiological concentrations.
       This may prove to be of therapeutic value in patients infected with
       HIV-1.
 DE    Cell Division/DRUG EFFECTS  Cell Survival/DRUG EFFECTS  Cells, Cultured
       Fetal Blood  Human  HIV-1/*DRUG EFFECTS/PHYSIOLOGY
       Insulin/*PHARMACOLOGY  Insulin-Like Growth Factor I/*PHARMACOLOGY
       Leukocytes, Mononuclear/CYTOLOGY/VIROLOGY  Support, Non-U.S. Gov't
       Virus Replication/*DRUG EFFECTS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

