       Document 0730
 DOCN  M9620730
 TI    In vitro stimulation of peripheral blood mononuclear cells (PBMC) from
       HIV- and HIV+ chancroid patients by Haemophilus ducreyi antigens.
 DT    9602
 AU    Van Laer L; Vingerhoets J; Vanham G; Kestens L; Bwayo J; Otido J; Piot
       P; Roggen E; Department of Infection and Immunity, Institute of
       Tropical; Medicine, Antwerp, Belgium.
 SO    Clin Exp Immunol. 1995 Nov;102(2):243-50. Unique Identifier : AIDSLINE
       MED/96069870
 AB    The cellular immune responses to fractionated Haemophilus ducreyi
       antigens, coated on latex beads, were assessed in patients with
       chancroid and in controls, using an in vitro lymphocyte proliferation
       assay. Several fractions of H. ducreyi antigen revealed stimulating
       activity. However, only the molecular size ranges 91-78 kD, 59-29 kD,
       and 25-21 kD induced proliferation that may be specifically related to
       H. ducreyi infection. Lymphocytes from four HIV- patients, successfully
       treated for chancroid, were not stimulated by H. ducreyi antigen. In
       general, lymphocytes from HIV+ chancroid patients were less responsive
       to H. ducreyi antigen compared with those from HIV- chancroid patients.
       However, two HIV-infected patients showed exceptionally strong responses
       to high molecular weight fractions. To our knowledge this is the first
       report demonstrating that H. ducreyi contains specific T
       cell-stimulating antigens. Based on this work, further identification
       and purification of the T cell antigens is feasible.
 DE    Antigens, Bacterial/IMMUNOLOGY  Chancroid/*COMPLICATIONS/IMMUNOLOGY
       Haemophilus ducreyi/IMMUNOLOGY  Human  HIV
       Infections/*COMPLICATIONS/IMMUNOLOGY  Lymphocyte Transformation  Male
       Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

