       Document 0233
 DOCN  M9620233
 TI    In vitro integration of human immunodeficiency virus type 1 cDNA into
       targets containing protein-induced bends.
 DT    9602
 AU    Bor YC; Bushman FD; Orgel LE; Salk Institute for Biological Studies, San
       Diego, CA 92186-8500,; USA.
 SO    Proc Natl Acad Sci U S A. 1995 Oct 24;92(22):10334-8. Unique Identifier
       : AIDSLINE MED/96036079
 AB    Integration of human immunodeficiency virus type 1 cDNA into a target
       DNA can be strongly influenced by the conformation of the target. For
       example, integration in vitro is sometimes favored in target DNAs
       containing sequence-directed bends or DNA distortions caused by bound
       proteins. We have analyzed the effect of DNA bending by studying
       integration into two well-characterized protein-DNA complexes:
       Escherichia coli integration host factor (IHF) protein bound to a phage
       IHF site, and the DNA binding domain of human lymphoid enhancer factor
       (LEF) bound to a LEF site. Both of these proteins have previously been
       reported to bend DNA by approximately 140 degrees. Binding of IHF
       greatly increases the efficiency of in vitro integration at hotspots
       within the IHF site. We analyzed a series of mutants in which the IHF
       site was modified at the most prominent hotspot. We found that each
       variant still displayed enhanced integration upon IHF binding. Evidently
       the local sequence is not critical for formation of an IHF hotspot. LEF
       binding did not create preferred sites for integration. The different
       effects of IHF and LEF binding can be rationalized in terms of the
       different proposed conformations of the two protein-DNA complexes.
 DE    Bacterial Proteins/CHEMISTRY/*METABOLISM  Base Sequence  Comparative
       Study  DNA Nucleotidyltransferases/BIOSYNTHESIS/*METABOLISM  DNA Primers
       DNA-Binding Proteins/METABOLISM  DNA,
       Complementary/CHEMISTRY/*METABOLISM  DNA, Viral/CHEMISTRY/*METABOLISM
       Human  HIV-1/*GENETICS/METABOLISM  Models, Structural  Molecular
       Sequence Data  Nucleic Acid Conformation  Polymerase Chain Reaction
       Protein Conformation  Recombinant
       Proteins/BIOSYNTHESIS/CHEMISTRY/METABOLISM  Support, U.S. Gov't, P.H.S.
       *Virus Integration  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

