       Document 0530
 DOCN  M9620530
 TI    Early IL-4 production by non-CD4+ cells at the site of antigen
       deposition predicts the development of a T helper 2 cell response to
       Schistosoma mansoni eggs.
 DT    9602
 AU    Sabin EA; Pearce EJ; Department of Microbiology and Immunology, College
       of Veterinary; Medicine, Cornell University, Ithaca, NY 14853, USA.
 SO    J Immunol. 1995 Nov 15;155(10):4844-53. Unique Identifier : AIDSLINE
       MED/96062303
 AB    Cytokines play a major role in promoting naive Th cells to differentiate
       into Th1 or Th2 cells. While IL-4 is recognized as the primary pro-Th2
       inducing cytokine, the identity of its cellular sources during the
       development of a Th2 response remains unclear. We have used Schistosoma
       mansoni eggs, potent stimulators of Th2 responses both during the
       natural progression of murine schistosomiasis and when experimentally
       isolated and injected into normal mice, to examine IL-4 production early
       in the evolution of an Ag-driven Th2 response. Analysis of peritoneal
       exudate cells by IL-4 specific reverse transcriptase-PCR and ELISPOT, at
       times following i.p. egg injection in naive C57BL/6 mice, revealed a
       marked, transient elevation in IL-4 production at 2 to 12 h after Ag
       exposure. This response was temporally accompanied by eosinophil and
       neutrophil infiltration and mast cell disappearance. The pattern of
       early IL-4 production and peritoneal cell infiltration was observed in
       egg-injected CD4+ cell-depleted and nude C57BL/6 mice, strongly
       suggesting that a non-T cell is the source of early IL-4 and that the
       stimulus leading to the egg-induced changes in cellular composition are
       T cell independent. In addition to IL-4 transcripts, peritoneal exudate
       cells from egg-injected T cell replete or deficient mice contained
       IFN-gamma and IL-12 transcripts. Control i.p. PBS injections led to no
       or minimal cytokine gene transcription. Early IL-4 was predictive of
       subsequent Th2 response development since, in contrast to C57BL/6 mice,
       egg-injected BALB/c mice demonstrated no detectable IL-4 production at
       12 h and mounted a comparatively weak egg Ag-specific Th2 response.
 DE    Animal  Antigens, CD4/IMMUNOLOGY  Base Sequence  Cell
       Differentiation/IMMUNOLOGY  Female  *Immunity, Cellular
       Interleukin-4/*BIOSYNTHESIS  Mice  Mice, Inbred BALB C  Mice, Inbred
       C57BL  Mice, Nude  Molecular Sequence Data  *Schistosoma mansoni
       Schistosomiasis/*IMMUNOLOGY  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, P.H.S.  Th2 Cells/*IMMUNOLOGY/PARASITOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

