       Document 0243
 DOCN  M9620243
 TI    Eimeria acervulina infection: evidence for the involvement of CD8+ T
       lymphocytes in sporozoite transport and host protection.
 DT    9602
 AU    Trout JM; Lillehoj HS; USDA, Agricultural Research Service, Beltsville,
       Maryland 20705,; USA.
 SO    Poult Sci. 1995 Jul;74(7):1117-25. Unique Identifier : AIDSLINE
       MED/96075754
 AB    Host-parasite interactions during coccidial infection are complex, and
       host responses leading to parasite elimination are not well understood.
       Interactions between host cells and parasites were investigated using
       two-color immunofluorescent staining of duodenal tissue infected with
       Eimeria acervulina. Tissues were removed from TK strain chickens 24, 48,
       and 72 h after primary infection with 2.5 x 10(7) normal or irradiated
       oocysts and also following secondary infection with 2.5 x 10(7) normal
       oocysts. The tissues were embedded in freezing compound, frozen,
       sectioned, and fixed in cold acetone. Sections were stained with
       monoclonal antibodies to identify CD4+, CD8+, Ig+, and Bu1a+
       lymphocytes, as well as macrophages and parasites. At 24 h after all
       infections, sporozoites were seen primarily in CD8+ lymphocytes and
       macrophages. Many CD8+ lymphocytes were seen at all times in infected
       tissues and frequently appeared to be in contact with infected
       epithelial cells. Sporozoites were occasionally seen in CD4+ lymphocytes
       but none were noted in Ig+ or Bu1a+ lymphocytes. Meront development was
       extensive after primary infection with normal oocysts, sparse after
       primary infection with irradiated oocysts, and moderate after secondary
       infection.
 DE    Animal  Antibodies, Monoclonal  *Chickens
       Coccidiosis/IMMUNOLOGY/PHYSIOPATHOLOGY/*VETERINARY  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  Eimeria/*IMMUNOLOGY  Host-Parasite Relations
       Kinetics  Photomicrography  Poultry Diseases/IMMUNOLOGY/*PARASITOLOGY
       Support, U.S. Gov't, Non-P.H.S.  Time Factors  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

