       Document 1040
 DOCN  M9621040
 TI    Inhibition of HIV type 1 infection of mononuclear phagocytes by
       anti-CD44 antibodies.
 DT    9602
 AU    Rivadeneira ED; Sauls DL; Yu Y; Haynes BF; Weinberg JB; Department of
       Pediatrics, VA Medical Center, Durham, North; Carolina 27705, USA.
 SO    AIDS Res Hum Retroviruses. 1995 May;11(5):541-6. Unique Identifier :
       AIDSLINE MED/96093888
 AB    Cellular CD4 is the primary membrane molecule that binds HIV-1 through
       interaction with viral gp120. Membrane glycolipids and cell adhesion
       molecules have also been noted to be involved in the interaction of
       HIV-1 with cells and in syncytium formation in infected cells. The
       purpose of this study was to determine the role of the cell adhesion
       molecule CD44 in HIV-1 infection of cells. Both normal blood monocytes
       and lymphocytes expressed CD44 as determined by flow cytometry using the
       anti-CD44 antibody A3D8. Anti-CD44 monoclonal antibodies A3D8, A1G3, and
       5F12 [ascites, purified IgG, and F(ab')2] inhibited infection of
       monocytes and peritoneal macrophages with HIV-1-BaL and HIV-1-ADA, but
       had no effect on HIV-1-IIIB infection of mitogen-stimulated lymphocytes,
       or cells of a T lymphocyte line. CD44 monoclonal antibodies were not
       toxic for monocytes, and the observed inhibitory effect of CD44
       monoclonal antibodies was not dependent on complement. These results
       suggest that CD44 may be a determinant of HIV-1 infection of mononuclear
       phagocytes in vitro.
 DE    Animal  Antibodies, Monoclonal/*IMMUNOLOGY  Antigens,
       CD44/IMMUNOLOGY/*PHYSIOLOGY  Cells, Cultured  Human  HIV-1/*PHYSIOLOGY
       Lymphocytes/VIROLOGY  Macrophages, Peritoneal/VIROLOGY  Mice
       Monocytes/*VIROLOGY  Support, Non-U.S. Gov't  Support, U.S. Gov't,
       Non-P.H.S.  Support, U.S. Gov't, P.H.S.  T-Lymphocytes/VIROLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

