       Document 0102
 DOCN  M9620102
 TI    A molecular and serologic study of the envelope gene of the British
       isolate: HIV-1 GB8.
 DT    9602
 AU    Vella C; Smith MH; Farrar GH; Jones DH; Daniels RS; Division of
       Virology, National Institute for Medical Research,; London, UK.
 SO    Vaccine. 1995;13(8):735-41. Unique Identifier : AIDSLINE MED/96100812
 AB    The entire envelope gene of a British HIV-1 isolate, GB8, was cloned,
       sequenced and aligned with those of the reference strains MN, SF2 and
       IIIB/LAI. Three of the viruses (MN, IIIB/LAI, GB8) and their recombinant
       gp120s, were then characterised using an extensive panel of human HIV-1
       positive sera and mapped neutralising monoclonal antibodies (MAbs).
       Overall, the GB8 env-gene translation product shares 84% homology with
       those of the reference strains. Across the V3 region homology was
       greater between GB8 and SF2/MN (74.3-88.6%) than IIIB/LAI (63.9-66.7%).
       Accordingly, GB8 was sensitive to V3-specific MAbs which neutralise
       MN/SF2 and resistant to those that neutralize IIIB/LAI. In the CD4
       binding region the central MWQEVGKAMYAPPI was conserved in all viruses
       but homology in the N-terminus was greater between GB8 and SF2 and
       IIIB/LAI than MN. GB8 and IIIB/LAI were sensitive to all MAbs raised
       against the CD4 binding site whereas MN was resistant to 3 of 4 tested.
       Human sera obtained from a London-based cohort did not differentiate
       between GB8 and MN in neutralisation assays, whereas IIIB/LAI titres
       were significantly lower at all stages of disease. These results show
       that GB8 carries a consensus-like V3 loop and is as representative as MN
       of HIV-1 viruses circulating in the UK. To our knowledge, GB8 is the
       only British HIV-1 isolate which has been characterised to date.
 DE    Amino Acid Sequence  Antibodies, Monoclonal/PHARMACOLOGY
       Antigen-Antibody Reactions  Base Sequence  Cells, Cultured  *Genes, env
       Great Britain  Human  HIV Envelope Protein gp120/GENETICS/IMMUNOLOGY
       HIV Seropositivity/IMMUNOLOGY  HIV-1/*GENETICS/IMMUNOLOGY/ISOLATION &
       PURIF  Immune Sera/PHARMACOLOGY  Molecular Sequence Data  Neutralization
       Tests  Peptides/IMMUNOLOGY  Recombinant Proteins/IMMUNOLOGY  Support,
       Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

