       Document 0815
 DOCN  M9620815
 TI    Increased titer of recombinant AAV vectors by gene transfer with
       adenovirus coupled to DNA-polylysine complexes.
 DT    9602
 AU    Mamounas M; Leavitt M; Yu M; Wong-Staal F; Department of Medicine,
       University of California, San Diego, La; Jolla 92093-0665, USA.
 SO    Gene Ther. 1995 Aug;2(6):429-32. Unique Identifier : AIDSLINE
       MED/96002920
 AB    One of the principal problems with using recombinant adeno-associated
       virus vectors (rAAV) as vehicles for gene delivery has been the
       difficulty in obtaining high-titer virus stocks after the initial
       transfection into producer cells. In this report we describe a method
       for transfecting cells at extremely high efficiency with rAAV vector DNA
       and complementation plasmid while simultaneously infecting those cells
       with replication competent adenovirus using adenovirus-polylysine-DNA
       complexes. We further show that this technique results in an increase in
       rAAV transducing titer by two orders of magnitude over what is typically
       achieved by standard calcium phosphate transfection.
 DE    Adenoviridae/*GENETICS/PHYSIOLOGY  Dependovirus/*GENETICS  Drug Carriers
       DNA, Viral/ADMINISTRATION & DOSAGE/METABOLISM  Genetic Complementation
       Test  *Genetic Vectors  Hela Cells  Human  HIV-1/*GENETICS  Plasmids
       Polylysine/ADMINISTRATION & DOSAGE  Recombination, Genetic  RNA,
       Catalytic/*BIOSYNTHESIS/GENETICS  Support, U.S. Gov't, P.H.S.
       Transfection/*METHODS  Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

