       Document 0658
 DOCN  M9620658
 TI    Longitudinal study of cytomegalovirus antibodies in individuals infected
       with the human immunodeficiency virus.
 DT    9602
 AU    Flo RW; Haukenes G; Nilsen A; Skjaerven R; Forsgren M; Fehniger TE;
       Medical Department B, Haukeland Hospital, University of Bergen,; Norway.
 SO    Eur J Clin Microbiol Infect Dis. 1995 Jun;14(6):504-11. Unique
       Identifier : AIDSLINE MED/96082794
 AB    Cytomegalovirus (CMV) antibody profiles were studied in 25 HIV-infected
       patients over periods of up to 56 months. Specific antibodies against
       CMV antigen components were monitored by complement-fixation (CF) test,
       EIA, Western blot and a neutralization assay. Three subjects remained
       CMV seronegative throughout the study. Marked fluctuations were observed
       in anti-CMV antibodies assayed by the CF test as compared to a control
       group. Fluctuations on immunoblots of purified virion antigens were also
       observed in the HIV-infected patients; neutralizing antibodies and
       anti-CMV nucleocapsid antibodies showed less variability. Seven of 22
       individuals exhibited an increase in CF-test titre of up to 64-fold
       without clinically apparent CMV disease. On Western-blot testing of IgG
       reactivity with disrupted virions, ten individuals exhibited increasing
       reactivity to pp65, and only three of these also showed a titre rise in
       the CF test. In contrast, 7 of 22 showed low reactivity to the pp28
       antigen. The homosexual patient group exhibited the highest levels of
       anti-CMV antibody. In conclusion, many asymptomatic HIV-infected
       subjects showed fluctuations at different levels of their antibody
       response to CMV, thought to be indicative of CMV
       reactivation/reinfection. Western-blot findings indicated that some CMV
       antibodies increased in level while others were lost.
 DE    Adult  Antibodies, Viral/*BLOOD  AIDS-Related Opportunistic
       Infections/DIAGNOSIS/*IMMUNOLOGY  Blotting, Western  Complement Fixation
       Tests  Cytomegalovirus/*IMMUNOLOGY  Cytomegalovirus
       Infections/DIAGNOSIS/*IMMUNOLOGY  Enzyme-Linked Immunosorbent Assay
       Female  Human  HIV Infections/*BLOOD  IgM/ANALYSIS  Longitudinal Studies
       Male  Serodiagnosis  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

