       Document 0609
 DOCN  M9620609
 TI    Cyclophilin A, the major intracellular receptor for the
       immunosuppressant cyclosporin A, maps to chromosome 7p11.2-p13: four
       pseudogenes map to chromosomes 3, 10, 14, and 18.
 DT    9602
 AU    Willenbrink W; Halaschek J; Schuffenhauer S; Kunz J; Steinkasserer A;
       Sandoz Research Institute, Vienna, Austria.
 SO    Genomics. 1995 Jul 1;28(1):101-4. Unique Identifier : AIDSLINE
       MED/96070439
 AB    Cyclophilin A (CyP-A), the major intracellular receptor for the
       immunosuppressant cyclosporin A (CsA), is a member of the immunophilin
       class of proteins, which all possess peptidyl-prolyl cis-trans isomerase
       activity and, therefore, are believed to be involved in protein folding
       and/or intracellular protein transport. The CyP-A protein is encoded by
       a single gene; in addition, 15 pseudogenes have been identified.
       Recently, specific binding of CyP-A to the human immunodeficiency virus
       type 1 (HIV-1) gag protein has been reported. Interestingly, this
       interaction can be inhibited by the immunosuppressant CsA and also by
       nonimmunosuppressive, CyP-A-binding CsA derivatives, which were also
       shown to exhibit potent anti-HIV-1 activity. Results thus indicate that
       CyP-A may have an essential function in HIV-1 replication. Using a panel
       of somatic rodent-human cell hybrids and PCR technology, we localized
       the coding cyclophilin A gene (PPIA) on chromosome 7 and four
       pseudogenes (PPIP2, PPIP3, PPIP4, and PPIP6) on chromosomes 14, 10, 18,
       and 3, respectively. Using chromosome 7 and chromosome 10 deletion
       hybrid panels, we were able to localize further the coding gene to the
       region 7p11.2-p13, as confirmed by fluorescence in situ hybridization
       analysis, and one pseudogene (PPIP3) to the region 10q11.2-q23. This is
       the first report on the regional mapping of members of the CyP-A gene
       family.
 DE    Amino Acid Isomerases/*GENETICS/METABOLISM  Animal  Base Sequence
       Carrier Proteins/*GENETICS/METABOLISM  *Chromosome Mapping  Chromosomes,
       Human, Pair 10  Chromosomes, Human, Pair 14  Chromosomes, Human, Pair 18
       Chromosomes, Human, Pair 3  *Chromosomes, Human, Pair 7
       Cyclosporine/METABOLISM  Human  Hybrid Cells  Immunosuppressive
       Agents/METABOLISM  Molecular Sequence Data  Pseudogenes  Support,
       Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

