       Document 0199
 DOCN  M9620199
 TI    Stochastic events in the amplification of HTLV-I integration sites by
       linker-mediated PCR.
 DT    9602
 AU    Cavrois M; Wain-Hobson S; Wattel E; Institut de Recherche sur le Cancer
       de Lille, Lille, France.
 SO    Res Virol. 1995 May-Jun;146(3):179-84. Unique Identifier : AIDSLINE
       MED/96075768
 AB    Human T-cell leukaemia virus type I (HTLV-I) proviral integration sites
       from an asymptomatic carrier and from the MT4 cell line were analysed by
       linker-mediated PCR (LMPCR) and inverse PCR (IPCR). LMPCR was more
       sensitive, allowing detection of a greater number of integrated
       proviruses. Reconstruction experiments using a cloned integrated HTLV-1
       provirus indicated that > 100 copies were necessary to be detected
       frequently by LMPCR. To circumvent this problem, the LMPCR analysis was
       performed approximately 20 times per sample. Thus, for the MT4 cell
       line, the seven major integration sites were accompanied by
       approximately 20 clones of lesser frequency. For an asymptomatic HTLV-I
       carrier, nine integration sites were identified in a single
       amplfication, while a further 9 followed from 14 additional reactions.
       These findings show that there is a stochastic element to sampling
       HTLV-I integration sites by LMPCR, which tends to underestimate the
       actual number of HTLV-I bearing clones. Accordingly, those detected in
       at least two reactions represent the most abundant clones.
 DE    Base Sequence  Cell Line  DNA  DNA, Viral  Human  HTLV-BLV
       Infections/GENETICS/*VIROLOGY  HTLV-I/*GENETICS  Molecular Sequence Data
       Polymerase Chain Reaction/*METHODS  Proviruses/GENETICS  Sensitivity and
       Specificity  Stochastic Processes  Support, Non-U.S. Gov't  *Virus
       Integration  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

