       Document 0676
 DOCN  M9620676
 TI    Lymphocyte subset determination using a hematology analyzer.
 DT    9602
 AU    Hudson JC; Brunhouse RF; Garrison C; Rodriguez CM; Zwerner R; Russell
       TR; Coulter Corporation, Miami, FL 33116-9015, USA.
 SO    Cytometry. 1995 Jun 15;22(2):150-3. Unique Identifier : AIDSLINE
       MED/96090299
 AB    Anti-CD4 antibody (T4)-coated microspheres were used to label CD4 cells
       in whole blood. The mixture was lysed and analyzed by a modified Coulter
       VCS hematology analyzer, which differentiated microsphere-labeled cells
       by a change in Coulter volume, conductance, and light scatter.
       %CD3+/CD4+ fluorescent values from a profile were compared to %CD4
       values using the VCS-microsphere method. CD3 gating was used to exclude
       CD4+ monocytes from the 90LS-FALS lymphocyte gate. The results
       correlated well (R = 0.996). The percentage of CD4+ lymphocytes from
       profile scatterplots and VCS scatterplots showed a line of regression
       close to the equivalence line (n = 76, slope = 0.96) when CD3 gating was
       used for the profile. These results suggest that CD3 gating, though
       necessary for 90LS-FALS scatterplots, may not be necessary for
       volume-conductance-light scatterplots.
 DE    Antibody Specificity  Autoanalysis/*INSTRUMENTATION  Binding,
       Competitive  Blood Donors  Cell Size  Comparative Study  Electric
       Conductivity  Female  *Flow Cytometry  Hematology/*INSTRUMENTATION
       Human  HIV Seropositivity/*IMMUNOLOGY  Light  *Lymphocyte Subsets  Male
       Microspheres  Scattering, Radiation  CLINICAL TRIAL  JOURNAL ARTICLE
       RANDOMIZED CONTROLLED TRIAL

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

