       Document 0827
 DOCN  M9620827
 TI    Early appearance of antibodies to simian immunodeficiency virus in
       saliva and serum of infected macaques.
 DT    9602
 AU    Meiller TF; Narayan O; Joag SV; Overholser CD Jr; Baltimore College of
       Dental Surgery, Dental School, University of; Maryland 21201, USA.
 SO    Clin Diagn Lab Immunol. 1995 Jul;2(4):489-91. Unique Identifier :
       AIDSLINE MED/96082431
 AB    Simian immunodeficiency virus (SIV) infection in macaques is an
       important animal model for human immunodeficiency virus infection in
       humans. This study evaluated the temporal development of antibodies to
       SIV in the parotid saliva of macaques inoculated with the virus and
       compared these findings with the development of antibodies to SIV in the
       animals' sera. Three animals (ages, 14, 18, and 18 years) were
       inoculated with the macrophagetropic strain SIVmac239. Prior to
       inoculation and at consecutive weekly intervals during a four-week
       period following the initial virus inoculations, parotid saliva and
       serum were collected from each animal. A fourth animal (age, 9 years)
       served as a negative control, and the fifth and sixth animals (ages, 2
       and 22 years) served as positive controls (6 and 18 months
       postinoculation, respectively) with SIVmac239. Saliva and serum samples
       were reacted against SIV antigen in Western blots (immunoblots) prepared
       in the standard fashion to determine the presence of antibodies. The
       reactions of these antigen-antibody complexes with biotinylated
       anti-human immunoglobulin A (IgA), IgM, and IgG and biotinylated
       anti-human secretory component (SC) determined the class of antibody
       present or the presence of SC in the original parotid saliva or serum
       samples. In infected animals, the IgM to SIV was detectable in serum and
       saliva at 13 days, and antiviral IgA and IgG in serum and saliva were
       detectable at 20 to 27 days postinoculation. The antibody to SC reacted
       to saliva from only two animals at 20 and 27 days, and long-term
       positive controls were positive for SC in saliva, indicating that either
       secretory IgA or secretory IgM was present in these samples.(ABSTRACT
       TRUNCATED AT 250 WORDS)
 DE    Animal  Antibodies, Viral/BIOSYNTHESIS/*BLOOD  Female  Macaca
       Saliva/*IMMUNOLOGY  Simian Acquired Immunodeficiency
       Syndrome/BLOOD/*IMMUNOLOGY  Support, U.S. Gov't, P.H.S.  SIV/*IMMUNOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

