       Document 0964
 DOCN  M9620964
 TI    Failure of culture and polymerase chain reaction to detect human
       immunodeficiency virus (HIV) in seronegative steady sexual partners of
       HIV-infected individuals.
 DT    9602
 AU    MacGregor RR; Dubin G; Frank I; Hodinka RL; Friedman HM; Department of
       Medicine, University of Pennsylvania Medical; Center, Philadelphia, USA.
 SO    Clin Infect Dis. 1995 Jul;21(1):122-7. Unique Identifier : AIDSLINE
       MED/96065624
 AB    Because of concern that steady sexual partners of patients infected with
       human immunodeficiency virus (HIV) may be infected despite negative
       results in tests for antibody to HIV, we studied 50 sexually active
       couples with discordant antibody results, assessing the agreement
       between these serological results and those obtained by p24 antigen
       testing, the polymerase chain reaction (PCR), and culture. Forty-nine of
       50 seropositive sexual partners were also positive for HIV by PCR; the
       remaining seropositive partner was positive by culture. All seronegative
       partners also had negative results in the other three tests. Moreover,
       seronegative partners continued to have negative results in all tests
       for a mean follow-up period of 17 months despite ongoing sexual
       relations with their seropositive partners. Seronegative infection was
       not documented in these partners at risk for sexual transmission of HIV.
       HIV-negative individuals in stable, monogamous sexual relationships with
       HIV-infected partners apparently do not have a high incidence of
       infection despite continued sexual exposure.
 DE    Adult  Blotting, Western  Cohort Studies  DNA, Viral/ANALYSIS  Female
       Human  HIV Antibodies/ANALYSIS  HIV Core Protein p24/ANALYSIS  HIV
       Infections/*DIAGNOSIS  *HIV Seronegativity  HIV Seropositivity
       HIV-1/GENETICS/IMMUNOLOGY/*PHYSIOLOGY  Immunoenzyme Techniques
       Leukocytes, Mononuclear/VIROLOGY  Male  Polymerase Chain Reaction
       Reproducibility of Results  *Sexual Partners  Support, U.S. Gov't,
       P.H.S.  Virus Cultivation  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

