       Document 0120
 DOCN  M9620120
 TI    In vitro infection of bone marrow-adherent cells by human
       immunodeficiency virus type 1 (HIV-1) does not alter their ability to
       support hematopoiesis.
 DT    9602
 AU    Marandin A; Canque B; Coulombel L; Gluckman JC; Vainchenker W; Louache
       F; Institut Gustave Roussy, INSERM U 362, Villejuif, France.
 SO    Virology. 1995 Oct 20;213(1):245-8. Unique Identifier : AIDSLINE
       MED/96036499
 AB    As an attempt to elucidate the pathogenesis of human immunodeficiency
       virus type 1 (HIV-1)-related cytopenia, the effects of infection of
       long-term primary bone marrow culture (LTBMC)-derived adherent cells on
       hematopoiesis were investigated. Productive infection could then be
       established only when using monocytotropic strains HIV-1Ba-L, HIV-1Ada,
       and HIV-1JR-FL but not with lymphocytotropic strain HIV-1LAI. Culture
       supernatants were tested for major cytokines involved in the regulation
       of hematopoiesis: neither IL-3 nor GM-CSF were detectable in the
       infected or noninfected cultures; in contrast, TGF-beta, TNF-alpha,
       MIP-1 alpha, Steel Factor, and IL-6 were detected at all times in
       established LTBMCs, but their levels were not consistently altered by
       virus replication. In vitro functional analysis by colony and long-term
       culture assays showed that HIV-1 infection failed to alter either the
       kinetics or the number of hematopoietic progenitors produced by the
       stromal layers; it did not interfere with the clonogenicity of
       exogeneous CD34+ cells in semisolid assays, and no difference was
       observed relative to the controls when HIV-1-infected stromal layers
       were tested for their ability to sustain long-term hematopoiesis. These
       results show that productive and sustained virus replication in the
       macrophage component of LTBMCs does not significantly alter the profile
       of major cytokines involved in regulating hematopoiesis, nor is it
       sufficient by itself for altering in vitro hematopoiesis under the
       baseline conditions used.
 DE    Bone Marrow/CYTOLOGY/*PHYSIOLOGY/VIROLOGY  Cells, Cultured  Culture
       Media/METABOLISM  Cytokines/METABOLISM  Hematopoiesis/*PHYSIOLOGY  Human
       HIV-1/*PHYSIOLOGY  Stem Cells/PHYSIOLOGY  Support, Non-U.S. Gov't  Virus
       Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

