       Document 0821
 DOCN  M9620821
 TI    Intracellular immunization of human T cells with a hairpin ribozyme
       against human immunodeficiency virus type 1.
 DT    9602
 AU    Yamada O; Yu M; Yee JK; Kraus G; Looney D; Wong-Staal F; Department of
       Medicine, University of California, San Diego, La; Jolla 92093-0665,
       USA.
 SO    Gene Ther. 1994 Jan;1(1):38-45. Unique Identifier : AIDSLINE
       MED/96050918
 AB    T-cell lines (Jurkat and Molt-4) were transduced with retroviral vectors
       containing a hairpin ribozyme that targets a conserved sequence in the
       5' transcribed leader sequence of human immunodeficiency virus (HIV)
       type 1. Stable cell lines were generated which constitutively and
       persistently expressed the ribozyme gene driven by either the Moloney
       retroviral long terminal repeat (LTR) or an internal human tRNA(val)
       promoter. There was no apparent deleterious effect of long-term ribozyme
       expression on cell proliferation or viability. Cells expressing ribozyme
       were resistant to challenge from diverse strains of HIV, including an
       uncloned clinical isolate. No reverse transcriptase activity or virus
       infectivity was detectable in the culture supernatants of Jurkat cells
       expressing the ribozyme driven by the tRNA(val) promoter up to 35 days
       after challenge with HIV-1/HXB2. Expression of the ribozyme also
       significantly decreased (by approximately 50- to 100-fold) the
       efficiency of incoming virus to synthesize viral DNA. These and
       previously reported results indicate that transfer and expression of the
       ribozyme gene interfere with both early and late events in the HIV
       replication cycle and confer long-term resistance to HIV-1 infection.
 DE    Base Sequence  Cell Line  DNA Primers/GENETICS  Gene Expression  Gene
       Therapy  Genetic Vectors  Human  HIV-1/GENETICS/*IMMUNOLOGY/PHYSIOLOGY
       *Immunization  Molecular Sequence Data  RNA,
       Catalytic/GENETICS/*IMMUNOLOGY  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, Non-P.H.S.  Support, U.S. Gov't, P.H.S.
       T-Lymphocytes/*IMMUNOLOGY  Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

