       Document 0201
 DOCN  M9620201
 TI    Inhibition of in vitro HIV infection by trinitrophenyl-protein
       conjugates.
 DT    9602
 AU    Matsiota-Bernard P; Guetard D; Rame V; Montagnier L; Avrameas S; Unite
       d'Immunocytochimie, Institut Pasteur, Paris, France.
 SO    Res Immunol. 1995 Feb;146(2):109-17. Unique Identifier : AIDSLINE
       MED/96100667
 AB    Levels of natural antibodies (NAb) with high anti-trinitrophenyl (TNP)
       activity are increased during human immunodeficiency virus (HIV)
       infection. The aim of the present study was to examine the anti-HIV
       effect of natural anti-TNP antibodies, as well as that of their internal
       image, TNP antigen, on HIV infection in vitro. The results obtained with
       anti-TNP antibodies, as assessed by syncytia formation, were variable,
       although they demonstrated an inhibitory effect. In contrast, using RT
       activity assay plus evaluation of syncytia formation and the viral
       cytopathic effect, we found that bovine serum albumin (BSA) bearing
       different TNP groups was able to inhibit HIV infection of peripheral
       mononuclear cells and T4 cell lines without affecting cell metabolism or
       proliferation. BSA alone was devoid of activity; the antiviral effect
       depended on TNP substitution of the BSA molecule, and passage through an
       anti-TNP immunoadsorbent abolished this effect. The mechanism by which
       TNP exerts this antiviral effect is unclear. Antigenic epitopes may be
       shared by HIV and TNP, since monoclonal antibodies directed against
       various HIV proteins reacted with TNP in an enzyme immunoassay. TNP-BSA,
       however, did not bind to the CD4 receptor.
 DE    Animal  Antibodies, Monoclonal/PHARMACOLOGY  Antiviral
       Agents/*PHARMACOLOGY  Cattle  Cells, Cultured  CD4-Positive
       T-Lymphocytes  Human  HIV Infections/*IMMUNOLOGY/PREVENTION & CONTROL
       Immune Sera/PHARMACOLOGY  Immunity, Natural/*IMMUNOLOGY  Leukocytes,
       Mononuclear  Serum Albumin/CHEMISTRY/*IMMUNOLOGY/PHARMACOLOGY
       Trinitrobenzenes/CHEMISTRY/*IMMUNOLOGY/PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

