       Document 0410
 DOCN  M9620410
 TI    Mucosal model of immunization against human immunodeficiency virus type
       1 with a chimeric influenza virus.
 DT    9602
 AU    Muster T; Ferko B; Klima A; Purtscher M; Trkola A; Schulz P; Grassauer
       A; Engelhardt OG; Garcia-Sastre A; Palese P; et al; Institut fur
       Angewandte Mikrobiologie, Universitat fur; Bodenkultur, Vienna, Austria.
 SO    J Virol. 1995 Nov;69(11):6678-86. Unique Identifier : AIDSLINE
       GENBANK/U26830
 AB    Previously, we constructed a chimeric influenza virus that expresses the
       highly conserved amino acid sequence ELDKWA of gp41 of human
       immunodeficiency virus type 1 (HIV-1). Antisera elicited in mice by
       infection with this chimeric virus showed neutralizing activity against
       distantly related HIV-1 isolates (T. Muster, R. Guinea, A. Trkola, M.
       Purtscher, A. Klima, F. Steindl, P. Palese, and H. Katinger, J. Virol.
       68:4031-4034, 1994). In the present study, we demonstrated that
       intranasal immunizations with this chimeric virus are also able to
       induce a humoral immune response at the mucosal level. The immunized
       mice had ELDKWA-specific immunoglobulins A in respiratory, intestinal,
       and vaginal secretions. Sustained levels of these secretory
       immunoglobulins A were detectable for more than 1 year after
       immunization. The results show that influenza virus can be used to
       efficiently induce secretory antibodies against antigens from foreign
       pathogens. Since long-lasting mucosal immunity in the genital and
       intestinal tracts might be essential for protective immunity against
       HIV-1, influenza virus appears to be a promising vector for
       HIV-1-derived immunogens.
 DE    Amino Acid Sequence  Animal  AIDS Vaccines/IMMUNOLOGY  Cells, Cultured
       Chimera  Conserved Sequence  Enzyme-Linked Immunosorbent Assay  Female
       Human  HIV Antibodies/BIOSYNTHESIS/*IMMUNOLOGY
       HIV-1/GENETICS/*IMMUNOLOGY  IgA/BIOSYNTHESIS/IMMUNOLOGY
       IgG/BIOSYNTHESIS/IMMUNOLOGY  Intestinal Mucosa/IMMUNOLOGY/VIROLOGY
       Lung/IMMUNOLOGY/VIROLOGY  Lymphocytes/*IMMUNOLOGY/VIROLOGY  Mice
       Molecular Sequence Data  Mucous Membrane/IMMUNOLOGY/VIROLOGY
       Neutralization Tests  Orthomyxovirus Type A, Human/GENETICS/*IMMUNOLOGY
       Spleen/IMMUNOLOGY/VIROLOGY  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, P.H.S.  Vagina/IMMUNOLOGY/VIROLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

