       Document 0630
 DOCN  M9620630
 TI    DNA synthesis primed by mononucleotides (de novo synthesis) catalyzed by
       HIV-1 reverse transcriptase: tRNA(Lys,3) activation.
 DT    9602
 AU    Zakharova OD; Tarrago-Litvak L; Fournier M; Litvak S; Nevinsky GA;
       Institute of Bioorganic Chemistry, Siberian Division of the; Academy of
       Sciences of Russia, Novosibirsk, Russian Federation.
 SO    FEBS Lett. 1995 Oct 16;373(3):255-8. Unique Identifier : AIDSLINE
       MED/96033984
 AB    HIV-1 RT is able to catalyze DNA synthesis starting from mononucleotides
       used both as minimal primers and as nucleotide substrates (de novo
       synthesis) in the presence of a complementary template. The rate of this
       process is rather slow when compared to the polymerization primed by an
       oligonucleotide. The addition of tRNA(Lys,3) to this system increased
       the de novo synthesis rate by 2-fold. Addition of low concentrations of
       agents able to modify protein conformation, such as urea,
       dimethylsulfoxide and Triton X-100, can activate the de novo synthesis
       by a factor 2 to 5. A dramatic synergy is observed in the presence of
       the three compounds since the stimulating effect of tRNA increases 10-15
       times. These results suggest that compounds activating RT are able to
       induce a conformational change of the enzyme which results in a higher
       specific activity. Primer tRNA seems to play an important role in HIV-1
       RT modification(s) leading to a polymerase having a higher affinity for
       the primer or the dTTP, but not for the template. The specificity of RT
       for the template is not influenced by changes in the kinetics or in the
       thermodynamic parameters of the polymerization reaction.
 DE    Deoxyribonucleotides/*METABOLISM  Dimethyl Sulfoxide/PHARMACOLOGY
       DNA/*BIOSYNTHESIS  Enzyme Activation  HIV-1/*ENZYMOLOGY
       Octoxynol/PHARMACOLOGY  Poly A/METABOLISM  Poly T/METABOLISM  Poly
       U/METABOLISM  Protein Conformation  RNA-Directed DNA
       Polymerase/CHEMISTRY/*METABOLISM  RNA, Transfer, Amino Acyl/*METABOLISM
       Support, Non-U.S. Gov't  Templates  Urea/PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

