       Document 0814
 DOCN  M9620814
 TI    Evaluation of retroviral vectors based on the gibbon ape leukemia virus.
 DT    9602
 AU    Eglitis MA; Schneiderman RD; Rice PM; Eiden MV; Unit of Molecular
       Virology, National Institute of Mental Health,; Bethesda, MD 20892-4068,
       USA.
 SO    Gene Ther. 1995 Sep;2(7):486-92. Unique Identifier : AIDSLINE
       MED/96050909
 AB    The gibbon ape leukemia viruses (GaLVs) are primate-derived C-type
       retroviruses with a broad host range. Using an infectious, full-length
       clone of the GaLV SEATO strain, we have determined that this virus
       replicates efficiently in 13 of 17 human cell lines tested. In fact, the
       SB lymphoblast cell line, while resistant to infection by wild-type
       amphotropic mouse leukemia virus (A-MLV), was infected by GaLV-SEATO. We
       constructed vectors containing GaLV components and compared the
       performance of genomes containing an enhancer and promoter derived
       either from the SEATO or SF strains of GaLV. The GaLV vector genomes
       were packaged in a Moloney (Mo)MLV core with either an A-MLV or GaLV
       SEATO envelope. We found that, in some cases, the vector genome appeared
       to be critical in obtaining optimal infection. For example, vectors with
       a GaLV SF-based genome infected the human HL60 cell line, whereas
       vectors with a GaLV SEATO-based genome did not. We also found that most,
       but not all, of the human cell lines tested were more susceptible to
       vectors packaged with the GaLV SEATO than A-MLV envelope. The source of
       the viral core was also important, in that some human cells appeared
       susceptible to infection only with GaLV genomes packaged in particles
       composed of a GaLV core and envelope. Our results show that GaLV-based
       packageable genomes can be expressed in target cells not efficiently
       infected by vectors containing MoMLV-based genomes. These results
       suggest that judicious combinations of retroviral genomes and structural
       components can significantly improve gene transfer into human cells.
 DE    Animal  Cell Line  DNA, Viral/GENETICS  Gene Expression  *Gene Transfer
       *Genetic Vectors  Hamsters  Human  Leukemia Virus, Gibbon Ape/*GENETICS
       Mice  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

