       Document 0385
 DOCN  M9620385
 TI    Emergence of virus escape mutants after immunization with epitope
       vaccine.
 DT    9602
 AU    Weidt G; Deppert W; Utermohlen O; Heukeshoven J; Lehmann-Grube F;
       Heinrich-Pette-Institut fur Experimentelle Virologie und; Immunologie an
       der Universitat Hamburg, Germany.
 SO    J Virol. 1995 Nov;69(11):7147-51. Unique Identifier : AIDSLINE
       MED/96013818
 AB    BALB/c and C57BL/6J mice were immunized with recombinant vaccines
       consisting of lymphocytic choriomeningitis virus CD8+ T-lymphocyte
       epitopes and a carrier protein. During challenge infection with WE
       strain lymphocytic choriomeningitis virus, mutants with alterations in
       distinct amino acid residues of the epitopic nonapeptides appeared and
       multiplied. Splenocytes from WE-infected BALB/c mice lysed cells coated
       with the WE-type epitope; lysis was considerably less effective when the
       epitopic nonapeptide with which the syngeneic cells had been sensitized
       was the mutated form. Neither target was lysed by splenocytes from
       BALB/c mice infected with the variant virus. Mutants were not detected
       in F1 hybrid mice immunized with two viral epitopes that were restricted
       by class I molecules of both parents.
 DE    Amino Acid Sequence  Animal  Base Sequence  Capsid/CHEMISTRY/IMMUNOLOGY
       CD8-Positive T-Lymphocytes/IMMUNOLOGY  DNA Primers  DNA, Complementary
       DNA, Viral/ANALYSIS/CHEMISTRY  Epitopes/*IMMUNOLOGY  Female  Lymphocytic
       Choriomeningitis Virus/*GENETICS/*IMMUNOLOGY/  ISOLATION & PURIF  Mice
       Mice, Inbred BALB C  Mice, Inbred C57BL  Mice, Inbred Strains  Molecular
       Sequence Data  Mutagenesis  Polymerase Chain Reaction  Support, Non-U.S.
       Gov't  *Vaccines, Synthetic  Viral Core Proteins/CHEMISTRY/IMMUNOLOGY
       *Viral Vaccines  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

