       Document 0067
 DOCN  M9620067
 TI    Long-term productive human immunodeficiency virus-1 infection in human
       infant microglia.
 DT    9602
 AU    Ioannidis JP; Reichlin S; Skolnik PR; Division of Geographic Medicine
       and Infectious Diseases, New; England Medical Center, Boston, MA 02111,
       USA.
 SO    Am J Pathol. 1995 Nov;147(5):1200-6. Unique Identifier : AIDSLINE
       MED/96065071
 AB    The course of human immunodeficiency virus 1 (HIV-1) infection in human
       infant microglia was studied using purified primary cultures of
       microglia derived from brain autopsy tissue. Previous in vitro studies
       have used fetal or adult brain tissue. Important differences may exist
       between brain tissues of different maturational ages with regard to
       HIV-1 replication and other neuropathogenic effects. Infant microglia
       were infected with four different strains of HIV-1 (JR-FL, JR-CSF, Ba-L,
       and IIIB). Productive infection was demonstrated by p24 antigen
       production, immunocytochemistry, and recovery of replication-competent
       virus from the supernatants of the infected cultures. Multinucleated
       giant cells developed in culture mimicking the neuropathological changes
       seen in the brains of patients with HIV encephalopathy. Productive
       infection was more readily established by monocyte-tropic strains (JR-FL
       and Ba-L) of HIV-1 than by a lymphocyte-tropic strain (IIIB). p24
       antigen production in this system peaked at 47 to 51 days postinfection.
       Viral persistence in giant cells was demonstrated by immunocytochemistry
       for the gp120 and gp41 viral antigens as late as 70 days postinfection.
       This in vitro culture system, using infant microglia that support viral
       replication for more than 2 months, may provide a useful model for
       studying the pathogenesis of progressive HIV encephalopathy.
 DE    AIDS Dementia Complex/ETIOLOGY/PATHOLOGY  Cells, Cultured  Cerebral
       Cortex/PATHOLOGY/VIROLOGY  Female  Granulocyte-Macrophage
       Colony-Stimulating Factor/PHARMACOLOGY  Human  HIV
       Infections/*PATHOLOGY/VIROLOGY  Infant  Microglia/DRUG
       EFFECTS/*PATHOLOGY/VIROLOGY  Support, U.S. Gov't, P.H.S.  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

