       Document 0150
 DOCN  M9620150
 TI    [Incidence and characterization of large-cell anaplastic lymphoma]
 DT    9602
 AU    Chirife AM; Schmitz L; Gimenez L; Marino L; de Maria H; Departamento de
       Inmunopatologia, Instituto de Oncologia Angel; H. Roffo, Universidad de
       Buenos Aires.
 SO    Sangre (Barc). 1995 Aug;40(4):275-9. Unique Identifier : AIDSLINE
       MED/96063458
 AB    PURPOSE: Large cell anaplastic lymphoma (LCAL) is a new entity among
       large cell lymphoma. Diagnosis is based upon morphology and on
       positivity to Ki1 antigen. The objectives of this study were: 1) to
       determine the incidence of LCAL in the patient population seen at the
       Instituto Roffo between 1981 and 1993; 2) to study the immunological
       phenotype; 3) to study the association with Epstein-Barr virus and HIV;
       4) To study the expression of oncogen bcl-2 and 5) to evaluate tumoral
       growth factor. MATERIAL AND METHODS: The study was done on 1030 biopsies
       of nodal and extranodal lymphoproliferative diseases, with Hodgkin and
       non-Hodgkin lymphoma. Of the 1030 consecutive cases, 67 were selected.
       They revealed pleomorphic cells with sinusoidal disposition. Biotin and
       avidin peroxidase techniques were used to identify the following
       antigens: CD30(Ki 1), CD45RO (UCHL1), CD20(L26), CD45(CL), Epstein Barr
       virus (VEB), human immunodeficiency virus (HIV), CD15, CD68, PCNA, bcl-2
       oncogen, vimentin, membrane epithelial antigen (EMA). Of these cases,
       only 10 revealed strong positive reaction to CD 30 (Ki1), thus they were
       considered to fulfill criteria to be classified as LCAL. RESULTS: 1.
       Incidence of LCAL in the lymphoma population under study was 1%. 2.
       Phenotype was B in 3 cases, T in 3, and macrophage in 1.3. There was 1
       case of positive EBV and 3 positive HIV. 4. Eight of 10 LCAL cases were
       positive for bcl-2 protein. 5. Tumoral growth factor was 57%. Also, it
       was noted that most cases were secondary to Hodgkin lymphoma, and a few
       secondary to polymorphic immunoblastic lymphoma, with negative reaction
       to CD15 and EMA and positive for Vimentin. CONCLUSIONS: 1. Incidence of
       LCAL amongst patients with lymphoma is very low. 2. Immunological
       phenotype is varied. 3. There was no significant association with EBV or
       HIV. 4. Oncogen bcl-2 was demonstrated in most cases. 5. There was a
       high percentage of cells in proliferation.
 DE    Adolescence  Adult  Aged  Aged, 80 and over  Antigens, CD/BIOSYNTHESIS
       Cell Division  Child  English Abstract  Female  G-Proteins/BIOSYNTHESIS
       Herpesvirus 4, Human/ISOLATION & PURIF  Human  HIV/ISOLATION & PURIF
       Immunophenotyping  Incidence  *Lymphoma, Large-Cell,
       Ki-1/EPIDEMIOLOGY/GENETICS/IMMUNOLOGY/  PATHOLOGY/VIROLOGY  Male  Middle
       Age  Proto-Oncogene Proteins/BIOSYNTHESIS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

