       Document 0835
 DOCN  M9620835
 TI    Oral fluid as a specimen for detection and confirmation of antibodies to
       human immunodeficiency virus type 1.
 DT    9602
 AU    Granade TC; Phillips SK; Parekh B; Pau CP; George JR; Division of
       HIV/AIDS, Centers for Disease Control and Prevention,; Atlanta, Georgia
       30333, USA.
 SO    Clin Diagn Lab Immunol. 1995 Jul;2(4):395-9. Unique Identifier :
       AIDSLINE MED/96082412
 AB    Paired serum and oral fluid specimens (n = 287) were collected with the
       Omni-Sal device and were assayed for the presence of antibodies to human
       immunodeficiency virus type 1 (HIV-1). Enzyme immunoassays
       (EIAs)--Abbott 3A11, an Organon Teknika Corporation research-use-only
       test, and the Murex GACELISA--were used per the manufacturers' inserts
       or were modified slightly to accommodate the oral fluid specimens.
       Compared with serum Western blot (immunoblot) results, each EIA had a
       sensitivity of 100% and the specificities were 89.6% for the Abbott 3A11
       EIA, 96.5% for the GACELISA, and 97.8% for the Organon Teknika
       Corporation EIA. Specificities based on specimens that were repeatedly
       reactive were 99.3% for all EIAs. A miniaturized Western blot technique
       used for confirmatory testing of both the serum and oral fluid specimens
       found 149 of the 287 samples to be HIV-1 antibody positive in both
       sample types. The Western blot banding patterns observed for the serum
       and oral fluid specimens were essentially identical. Immunoglobulin G
       concentrations were determined for all oral fluid specimens and ranged
       from < 0.5 to > 40.0 micrograms/ml. Immunoglobulin G concentrations did
       not correlate with the ability of any of the EIAs to detect
       HIV-1-specific antibody or with the ability of the modified Western blot
       to detect HIV-1 protein-specific antibodies.
 DE    Adolescence  Adult  Blotting, Western  Enzyme-Linked Immunosorbent Assay
       Female  Human  HIV Antibodies/*ANALYSIS/BLOOD  HIV Infections/IMMUNOLOGY
       HIV-1/*IMMUNOLOGY  IgG/ANALYSIS  Male  Middle Age  Saliva/*IMMUNOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

