       Document 0727
 DOCN  M9620727
 TI    The restricted IgG1 antibody response to maedi visna virus is seen
       following infection but not following immunization with recombinant gag
       protein.
 DT    9602
 AU    Bird P; Reyburn HT; Blacklaws BA; Allen D; Nettleton P; Yirrell DL; Watt
       N; Sargan D; McConnell I; Department of Veterinary Pathology, University
       of Edinburgh, UK.
 SO    Clin Exp Immunol. 1995 Nov;102(2):274-80. Unique Identifier : AIDSLINE
       MED/96069875
 AB    Maedi-visna (MVV) is a retrovirus of the subfamily lentivirinae which
       includes HIV, simian immunodeficiency virus (SIV) and feline
       immunodeficiency virus (FIV). Infection of its natural host, the sheep,
       does not cause overt immunodeficiency, but rather a chronic inflammatory
       disease. However, subtle immunological changes following infection have
       been reported including a sheep IgG1 subclass-restricted
       MVV-neutralizing antibody. Here we demonstrate by Western blotting that
       there is no IgG2 serum antibody response to any MVV antigen after MVV
       infection, in contrast to infection with the parapox virus Orf, when
       serum IgG2 anti-Orf antibody is readily detected. By ELISA, the IgG1
       antibody titres to Orf are higher than to MVV, but the minimum MVV serum
       antibody IgG1/IgG2 ratio is significantly raised compared with that for
       Orf virus antibody in the same sheep, indicating that the IgG2 defect in
       MVV infection cannot be accounted for by differences in the sensitivity
       of the Orf and MVV ELISA. Serum IgG2 anti-MVV gag p. 25 can be detected
       in both normal and MVV-infected sheep following immunization with
       purified recombinant MVV gag p 25 protein in Freund's complete adjuvant.
       The failure to make an IgG2 MVV-specific antibody indicates that
       immunological dysfunction can arise with macrophage tropic lentiviruses,
       and it may aid viral persistence.
 DE    Animal  Antibodies, Viral/*BIOSYNTHESIS  Antigens, Viral/IMMUNOLOGY
       Gene Products, gag/*IMMUNOLOGY  IgG/*BIOSYNTHESIS  Immunization
       Immunoglobulin Isotypes/IMMUNOLOGY  Orf Virus/IMMUNOLOGY  Recombinant
       Proteins/IMMUNOLOGY  Sheep  Sheep Diseases/IMMUNOLOGY  Support, Non-U.S.
       Gov't  Time Factors  Visna/*IMMUNOLOGY  Visna-Maedi Virus/*IMMUNOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

