       Document 0646
 DOCN  M9620646
 TI    CD40 ligand-positive CD8+ T cell clones allow B cell growth and
       differentiation.
 DT    9602
 AU    Hermann P; Van-Kooten C; Gaillard C; Banchereau J; Blanchard D;
       Schering-Plough, Laboratory for Immunological Research, Dardilly,;
       France.
 SO    Eur J Immunol. 1995 Oct;25(10):2972-7. Unique Identifier : AIDSLINE
       MED/96062053
 AB    A fraction of activated CD8+ T cells expresses CD40 ligand (CD40L), a
       molecule that plays a key role in T cell-dependent B cell stimulation.
       CD8+ T cell clones were examined for CD40L expression and for their
       capacity to allow the growth and differentiation of B cells, upon
       activation with immobilized anti-CD3. According to CD40L expression,
       CD8+ clones could be grouped into three subsets. CD8+ T cell clones
       expressing high levels of CD40L (> or = 80% CD40L+ cells) were
       equivalent to CD4+ T cell clones with regard to induction of tonsil B
       cell proliferation and immunoglobulin (Ig) production, provided the
       combination of interleukin (IL)-2 and IL-10 was added to cultures. CD8+
       T cell clones, with intermediate levels of CD40L expression (10 to 30%
       CD40L+ cells), also stimulated B cell proliferation and Ig secretion
       with IL-2 and IL-10. B cell responses induced by these CD8+ T cell
       clones were neutralized by blocking monoclonal antibodies specific for
       either CD40L or CD40. By contrast, CD40L- T cell clones (< or = 5%
       CD40L+ cells), only induced marginal B cell responses even with IL-2 and
       IL-10. All three clone types were able to activate B cells as shown by
       up-regulation of CD25, CD80 and CD86 expression. A neutralizing
       anti-CD40L antibody indicated that T cell-dependent B cell activation
       was only partly dependent on CD40-CD40L interaction. These CD40L- clones
       had no inhibitory effects on B cell proliferation induced by
       CD40L-expressing CD8+ T cell clones. Taken together, these results
       indicate that CD8+ T cells can induce B cell growth and differentiation
       in a CD40L-CD40-dependent fashion.
 DE    Animal  Antibody Formation  Antigens, CD26/BIOSYNTHESIS  Antigens,
       CD80/BIOSYNTHESIS  B-Lymphocytes/*CYTOLOGY/IMMUNOLOGY  Cell
       Differentiation  Cell Division  Cells, Cultured  Clone Cells/IMMUNOLOGY
       CD8-Positive T-Lymphocytes/*IMMUNOLOGY/METABOLISM  Flow Cytometry  Human
       Interleukin-10/PHARMACOLOGY  Interleukin-2/PHARMACOLOGY/SECRETION  L
       Cells  *Lymphocyte Cooperation  Lymphocyte Transformation  Membrane
       Glycoproteins/GENETICS/*PHYSIOLOGY  Mice  Mitogens/PHARMACOLOGY
       Phytohemagglutinins/PHARMACOLOGY  Receptors, Interleukin-2/BIOSYNTHESIS
       Support, Non-U.S. Gov't  T-Lymphocyte Subsets/*IMMUNOLOGY/METABOLISM
       Tonsil/CYTOLOGY  Transfection  Up-Regulation (Physiology)/DRUG EFFECTS
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

