       Document 1076
 DOCN  M9621076
 TI    Characteristics of perforin expressing lymphocytes within the first
       trimester decidua of human pregnancy.
 DT    9602
 AU    Rukavina D; Rubesa G; Gudelj L; Haller H; Podack ER; Department of
       Physiology and Immunology, Medical Faculty,; University of Rijeka,
       Croatia.
 SO    Am J Reprod Immunol. 1995 May;33(5):394-404. Unique Identifier :
       AIDSLINE MED/96070201
 AB    PROBLEM: The number of perforin (P)-positive cells in decidua of
       pregnancy is larger than that observed in any other pathological
       condition. The aim was to investigate the distribution and the phenotype
       of P+ cells. METHOD: Decidual tissue was obtained from the first
       trimester vaginal termination of pregnancy. Tissue distribution of P+
       cells was analyzed by immunohistochemistry. The method for simultaneous
       measurement of P and cell surface is presented. RESULTS: There is no
       difference in number and distribution of P+ cells between decidua
       basalis (DB) and decidua parietalis (DP). The percentage of P+ decidual
       lymphocytes (DL) is two times higher than in peripheral blood
       lymphocytes (PBL) (55% vs. 27%), and the prevalent phenotype is CD3-
       CD4- CD8- CD2+ (95%) CD11c+ (68%) and CD56+ (82%). CD56bright+ DL are
       also Pbright+ and this is the largest DL subpopulation (42.4% DL). Two
       different subpopulations of CD8+ DL exist: 1) CD8bright+, which are CD3+
       CD56- P- and 2) CD8dim+, which are CD3- CD56+ P+. CONCLUSION: P
       expressing DL are prevalently nonclassical NK cells (CD16-) with low
       cytolytic activity but fully equipped with potent cytolytic machinery
       (Pbright+). There are no classical cytotoxic lymphocytes (CTL) (CD3+
       CD8+ P+) in the decidua, and all CD8+ P+ cells are CD3- CD56+. The
       number of P+ cells is even higher in DP in the vicinity of noninvasive
       trophoblast, than in DB.
 DE    Antigens, CD56/ANALYSIS  Antigens, CD8/ANALYSIS  Biological
       Markers/ANALYSIS  CD8-Positive T-Lymphocytes/CHEMISTRY
       Decidua/*IMMUNOLOGY  Female  Human  Immunophenotyping  Killer Cells,
       Natural/CHEMISTRY  Membrane Glycoproteins/*ANALYSIS  Pregnancy
       Pregnancy Trimester, First/*IMMUNOLOGY  Staining  Support, Non-U.S.
       Gov't  T-Lymphocytes/*CHEMISTRY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

