       Document 0210
 DOCN  M9620210
 TI    [Optimization of the immunoelectrophoresis technic (western blot) for
       the confirmation of human immunodeficiency virus infection (HIV) in
       Panama]
 DT    9602
 AU    Pascale JM; de Austin E; de Moreno NO; Ledezma C; de Marquez E; Blanco
       R; Quiroz E; Calzada J; Vincensini AR; de Martin MC; et al; Departamento
       de Microbiologia, Facultad de Medicina, Universidad; de Panama.
 SO    Rev Med Panama. 1995 Jan-May;20(1-2):58-64. Unique Identifier : AIDSLINE
       MED/96046189
 AB    The purpose of this study is to report the results of the authors'
       investigation to apply the western blot technique (WB UP-LCS) in the
       diagnosis of human immunodeficiency virus type 1 (HIV-1) infection. To
       do this, the authors separated the proteins of the HIV-1 virus by
       electrophoresis, based on their molecular weight, in poliacilamide gel
       with SDS (SDS-PAGE) during 3 hours at 200 volts. Then they
       electrotransferred these proteins to nitrocellulose paper during four
       hours at 200 milliamperes, with the aid of external cooling. The
       nitrocellulose strips were evaluated considering the incubation time (1
       and 16 hours), two conjugates (human anti IgG with Peroxidase and human
       anti IgG Biotin plus Streptatividine with Peroxidase) and two dilutions
       of the patients' sera (1/50 and 1/100). Based on their results the
       Authors conclude that, in the first place, the optimal conditions for
       the test include a dilution of 1/100 of the patients serum, incubation
       of the serum for 16 hours and the use of the conjugate of anti human IgG
       with Biotin and Streptavidine with Peroxidase; secondary, that the
       immunologic reactivity against proteins p24 and gp 160/120 is the most
       important diagnostic criterion for the confirmation of infection with
       HIV-1 and that they obtained a diagnostic correlation of 100% at a cost
       which was 5 to 7 times less than that of the commercial system.
 DE    Blotting, Western/*METHODS/STATISTICS & NUMER DATA  Comparative Study
       Electrophoresis, Polyacrylamide Gel/METHODS  English Abstract
       Evaluation Studies  Human  HIV Antibodies/*BLOOD  HIV
       Infections/*DIAGNOSIS  *HIV-1  Time Factors  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

