       Document 0100
 DOCN  M9620100
 TI    Priming of class I-restricted cytotoxic T lymphocytes by vaccination
       with recombinant protein antigens.
 DT    9602
 AU    Schirmbeck R; Deml L; Melber K; Wolf H; Wagner R; Reimann J; Institute
       for Medical Microbiology, University of Ulm, Germany.
 SO    Vaccine. 1995 Jun;13(9):857-65. Unique Identifier : AIDSLINE
       MED/96066235
 AB    We investigated the specific priming of MHC class I-restricted cytotoxic
       T lymphocytes (CTL) by different protein antigen preparations in mice.
       The recombinant viral protein antigens tested are of potential relevance
       for the design of subunit vaccines. They include the hepatitis B virus
       (HBV) surface antigen (S-antigen), the HIV-1 gp160 envelope protein, and
       a chimeric HIV-1 Pr55-gag/V3-3 retrovirus-like particle. In addition,
       ovalbumin (OVA) was tested. The native or denatured particulate
       (multimeric) or monomeric form of these protein antigens was injected by
       various routes into mice. Class I-restricted CTL were efficiently primed
       by a single low-dose injection of HBV S-antigen particles or the
       chimeric HIV-1 Pr55-gag/V3-3 particles. After SDS-denaturation,
       gel-purified monomeric S-antigen and monomeric Pr55-gag/V3-3 fusion
       protein were still very efficient in priming CTL. CTL sensitization was
       not detected in a (primary or boosted) response to even high doses of
       native OVA or native HIV-1 gp160. Denaturation of these two antigens by
       detergent strikingly increased their immunogenicity for CTL.
       Immunization of mice with non-treated or SDS-denatured antigenic
       peptides representing the relevant CTL-defined epitopes of the tested
       protein antigens did not prime CTL. These data indicate that native,
       particulate and denatured, monomeric protein antigens efficiently
       stimulate a class I-restricted CTL response.
 DE    Amino Acid Sequence  Animal  Base Sequence  Detergents  DNA Primers
       Gene Products, env/CHEMISTRY/*IMMUNOLOGY  Gene Products,
       gag/GENETICS/IMMUNOLOGY  Hepatitis B Surface
       Antigens/CHEMISTRY/*IMMUNOLOGY  Histocompatibility Antigens Class
       I/*IMMUNOLOGY  HIV Envelope Protein gp120/GENETICS/IMMUNOLOGY
       HIV-1/*IMMUNOLOGY  Mice  Mice, Inbred C57BL  Molecular Sequence Data
       Ovalbumin/IMMUNOLOGY  Peptide Fragments/GENETICS/IMMUNOLOGY  Protein
       Denaturation  Protein Precursors/CHEMISTRY/GENETICS/*IMMUNOLOGY
       Recombinant Proteins/CHEMISTRY/IMMUNOLOGY  Spodoptera  Support, Non-U.S.
       Gov't  T-Lymphocytes, Cytotoxic/*IMMUNOLOGY  Tumor Cells, Cultured
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

