       Document 0728
 DOCN  M9620728
 TI    Spontaneous proliferation of memory (CD45RO+) and naive (CD45RO-)
       subsets of CD4 cells and CD8 cells in human T lymphotropic virus (HTLV)
       infection: distinctive patterns for HTLV-I versus HTLV-II.
 DT    9602
 AU    Prince HE; York J; Owen SM; Lal RB; American Red Cross Blood Services,
       Southern California Region,; Los Angeles.
 SO    Clin Exp Immunol. 1995 Nov;102(2):256-61. Unique Identifier : AIDSLINE
       MED/96069872
 AB    Spontaneous lymphocyte proliferation (SLP) in vitro is a characteristic
       feature of about 50% of individuals infected with HTLV-I or HTLV-II.
       Both CD4 cells and CD8 cells contribute to SLP in HTLV-I infection,
       whereas SLP in HTLV-II infection is usually restricted to CD8 cells. In
       this study, we asked if SLP was restricted to the memory (CD45RO+) cell
       subset of CD4 and CD8 cells in HTLV infection. Purified CD4 and CD8
       cells were separated into CD45RO+ and CD45RO- populations by a modified
       panning technique, and spontaneous proliferation (SP) of the cell
       subsets was assessed. For all five HTLV-I-infected persons whose
       mononuclear cell cultures were SLP+, only CD45RO+ cells, but not CD45RO-
       cells, within CD4 and CD8 subsets showed SP. In contrast, five of six
       SLP+ HTLV-II+ individuals showed SP in both the CD45RO+ and the CD45RO-
       subsets of CD4 cells, and 10 of 12 SLP+ HTLV-II+ individuals showed SP
       of both the CD45RO+ and CD45RO- subsets of CD8 cells. Polymerase chain
       reaction studies showed that proviral genome was generally present in
       both CD45RO+ and CD45RO- subsets of CD4 and CD8 cells, regardless of
       HTLV type and SP activity. These findings show that SP of both CD4 and
       CD8 cells in HTLV-I infection is usually restricted to CD45RO+ memory
       cells, whereas in HTLV-II infection, both CD45RO+ memory and CD45RO-
       naive subsets of CD4 and CD8 cells may exhibit SP. It thus appears that
       HTLV-I infection and HTLV-II infection exhibit distinctive dysregulatory
       effects on memory and naive T cell subpopulations.
 DE    Antigens, CD45/*METABOLISM  CD4-Positive T-Lymphocytes/*IMMUNOLOGY
       CD8-Positive T-Lymphocytes/*IMMUNOLOGY  Flow Cytometry  Human
       HTLV-I/*IMMUNOLOGY  HTLV-I Infections/*IMMUNOLOGY  HTLV-II/*IMMUNOLOGY
       HTLV-II Infections/*IMMUNOLOGY  *Immunologic Memory  *Lymphocyte
       Transformation  T-Lymphocyte Subsets/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

