       Document 0097
 DOCN  M9620097
 TI    Diagnosis of Pneumocystis carinii pneumonia by bronchoalveolar lavage in
       AIDS patients. Comparison of Diff-Quik, fungifluor stain, direct
       immunofluorescence test and polymerase chain reaction.
 DT    9602
 AU    Armbruster C; Pokieser L; Hassl A; IInd Medical Department, University
       of Vienna, Austria.
 SO    Acta Cytol. 1995 Nov-Dec;39(6):1089-93. Unique Identifier : AIDSLINE
       MED/96074702
 AB    OBJECTIVE: To assess the sensitivity, specificity and accuracy of
       Diff-Quik, fungifluor stain, the direct immunofluorescence test (DIFT)
       and the polymerase chain reaction (PCR) in the diagnosis of Pneumocystis
       carinii pneumonia (PCP) in human immunodeficiency virus (HIV)-infected
       patients. STUDY DESIGN: From December 1992 through November 1993, 112
       bronchoalveolar lavage fluid (BALF) samples were obtained from 80
       HIV-infected patients. BALF samples were processed for cytologic and
       microbiologic analysis and for PCR. Cytologic examination was carried
       out on Diff-Quik-stained cytocentrifuge preparations and with
       May-Grunwald-Giemsa staining and fungifluor staining. For diagnosis of
       PC infection, DIFT and PCR were used. RESULTS: Thirty-two of 112 acute
       episodes were caused by P carinii. Diff-Quik had the highest sensitivity
       (84.8%) as compared to fungifluor stain (60.0%), DIFT (59.4%) and PCR
       (65.6%). The specificity was 98.7% with Diff-Quik, 100% with fungifluor
       stain, and 98.6% and 97.3% with DIFT and PCR, respectively. Accuracy was
       high with every method (94.4% with Diff-Quik, 88.3% with fungifluor
       stain, 86.7% with DIFT and 87.6% with PCR). CONCLUSION: Diff-Quik is a
       good diagnostic tool in the diagnosis of PCP. The combination of
       Diff-Quik and fungifluor stain is recommended because of its
       cost-effectiveness and because of its rapid diagnosis of severe PCP. PCR
       and DIFT should be used only on patients judged clinically to have PCP
       with discrepant results in Diff-Quik and fungifluor stain in BALF
       samples.
 DE    AIDS-Related Opportunistic Infections/*DIAGNOSIS  *Bronchoalveolar
       Lavage  Comparative Study  Fluorescent Antibody Technique, Direct  Human
       Pneumonia, Pneumocystis carinii/*DIAGNOSIS  Polymerase Chain Reaction
       Sensitivity and Specificity  Staining  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

