       Document 0831
 DOCN  M9620831
 TI    Differential effects of human immunodeficiency virus type 1 envelope
       protein gp120 on interferon production by mononuclear cells from adults
       and neonates.
 DT    9602
 AU    Nair MP; Chadha KC; Stadler I; Sweet A; Schwartz SA; Department of
       Medicine and Microbiology, State University of New; York at Buffalo,
       USA.
 SO    Clin Diagn Lab Immunol. 1995 Jul;2(4):434-8. Unique Identifier :
       AIDSLINE MED/96082419
 AB    While considerable progress in examining the course of human
       immunodeficiency virus (HIV) infection in adults has been made, a better
       understanding of the natural history of perinatal HIV infection remains
       to be obtained. Dysregulation of the production and functions of various
       cytokines, especially the interferons (IFNs), during HIV infections has
       been reported. Using an in vitro model system, we examined the effects
       of the HIV type 1 envelope protein, gp120 (10, 50, and 100 ng/ml), on
       gamma IFN (IFN-gamma) and IFN-alpha production by lymphocytes from
       neonates and adults and also examined the potential regulatory effects
       of gp120 on phorbol 12-myristate acetate (PMA)- and Sendai virus-induced
       IFN-gamma and IFN-alpha production by lymphocytes. PMA at a
       concentration of 50 ng/ml plus 50 ng of calcium ionophore A23187 per ml
       was used to induce IFN-gamma, while 150 hemagglutinating units of Sendai
       virus was used to induce IFN-alpha production. The antiviral activity of
       both IFN-alpha and IFN-gamma in leukocyte culture supernatants was
       assayed on BG-9 cells by a dye uptake technique using vesicular
       stomatitis virus as a challenge virus. Placental cord blood leukocyte
       (CBL) samples from healthy, term infants and adult peripheral blood
       leukocytes (APBL) produced no IFN in response to gp120. However, CBL
       produced significantly decreased levels of IFN-gamma compared with APBL
       in response to PMA plus ionophore. gp120 significantly suppressed both
       Sendai virus-induced IFN-alpha and PMA-induced IFN-gamma production by
       both CBL and APBL in a dose-dependent manner. However, gp120-induced
       suppression of IFN-alpha and IFN-gamma was significantly greater with
       CBL than with APBL.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Adult  Antigens, CD45/DRUG EFFECTS  Comparative Study  Female  Human
       HIV Envelope Protein gp120/*PHARMACOLOGY  Immunophenotyping
       Immunosuppressive Agents/PHARMACOLOGY  Infant, Newborn  Interferon Type
       II/BIOSYNTHESIS/DRUG EFFECTS  Interferon-alpha/ANTAGONISTS &
       INHIB/*BIOSYNTHESIS/*DRUG EFFECTS  Ionophores/PHARMACOLOGY  Leukocytes,
       Mononuclear/DRUG EFFECTS/*METABOLISM/VIROLOGY  Lymphocytes/DRUG
       EFFECTS/METABOLISM  Male  Parainfluenza Virus Type 1/DRUG
       EFFECTS/IMMUNOLOGY  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       Tetradecanoylphorbol Acetate/PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

