       Document 0271
 DOCN  M9620271
 TI    Partial activation of CD8+ T cells by a self-derived peptide.
 DT    9602
 AU    Cao W; Tykodi SS; Esser MT; Braciale VL; Braciale TJ; Beirne B. Carter
       Center for Immunology Research, University of; Virginia Health Sciences
       Center, Charlottesville 22908, USA.
 SO    Nature. 1995 Nov 16;378(6554):295-8. Unique Identifier : AIDSLINE
       MED/96069766
 AB    T cells are normally activated when the peptide for which they are
       specific is presented to them in the context of the appropriate major
       histocompatibility complex (MHC) (class I and Class II for CD8+ and CD4+
       T cells, respectively). An increasing body of evidence indicates that
       structural homologues of the immunogenic peptide can partially activate
       or antagonize CD4+ T cells. CD8+ T cells may also be partially
       antagonized by such peptides, and self-derived peptides of this type may
       play a role in CD8+ T cell selection in the thymus. Activated CD8+ T
       cells lyse their targets by perforin-dependent granule exocytosis and by
       inducing apoptosis mediated by CD95 (also known as Fas or APO1) with its
       ligand (CD95L). Here we show that a clone of Kd-restricted CD8+ T cells
       specific for influenza haemagglutinin, which can also be activated in a
       crossreactive manner by a peptide derived from a myeloma tumour
       immunoglobulin heavy-chain variable region (IgVH) to kill by both
       routes, kills only by the CD95-CD95L pathway when stimulated by the
       corresponding germline IgVH peptide. As this germline IgVH peptide
       differs from the tumour peptide only at a single position buried in the
       MHC-binding groove, this indicates that CD95-CD95L-mediated killing can
       be triggered independently of the perforin-mediated pathway, and can be
       selectively affected by changes in MHC conformation.
 DE    Amino Acid Sequence  Animal  Antigens, CD45/IMMUNOLOGY  Base Sequence
       Cell Death  Cell Line  Clone Cells  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  DNA  H-2 Antigens/IMMUNOLOGY  Hemagglutinins,
       Viral/IMMUNOLOGY  Immunoglobulins, Heavy-Chain/GENETICS/*IMMUNOLOGY
       *Lymphocyte Transformation  Membrane Glycoproteins/IMMUNOLOGY  Mice
       Molecular Sequence Data  Myeloma Proteins/GENETICS/*IMMUNOLOGY  Peptide
       Fragments/*IMMUNOLOGY  Receptors, Antigen, T-Cell/IMMUNOLOGY  Signal
       Transduction  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

