       Document 0466
 DOCN  M9620466
 TI    Infection of Macaca nemestrina brain with human immunodeficiency virus
       type 1.
 DT    9602
 AU    Frumkin LR; Patterson BK; Leverenz JB; Agy MB; Wolinsky SM; Morton WR;
       Corey L; Department of Medicine, University of Washington School of;
       Medicine, Seattle 98195, USA.
 SO    J Gen Virol. 1995 Oct;76 ( Pt 10):2467-76. Unique Identifier : AIDSLINE
       GENBANK/K03455
 AB    We previously reported that pigtailed macaques (Macaca nemestrina)
       became infected after intravenous inoculation with the LAI strain of
       human immunodeficiency virus type 1 (HIV-1), an isolate that replicates
       in both M. nemestrina lymphocytes and blood-derived
       monocyte/macrophages. In the current study we investigated the presence
       of HIV-1 and pathology in the postmortem brains of four of these
       macaques. Histopathological findings revealed focal lesions in white
       matter in the frontal and occipital lobes of one macaque, with myelin
       loss, nerve fibre loss, and gliosis within these lesions.
       Semi-quantitative, solution-based PCR revealed HIV-1 DNA in the brains
       of two of the other macaques. Using slide-based PCR-driven in situ
       hybridization, we studied these two macaques further and detected
       intranuclear, circular HIV-1 DNA in vascular endothelia and other
       non-neuronal brain cells. These findings indicate that M. nemestrina
       brain can be infected with HIV-1 in vivo and may provide a useful animal
       model for understanding early HIV-1 brain infection in humans.
 DE    Amino Acid Sequence  Animal  AIDS Dementia Complex/*PATHOLOGY/*VIROLOGY
       Base Sequence  Brain/PATHOLOGY/*VIROLOGY  Cells, Cultured  Consensus
       Sequence  Disease Models, Animal  DNA, Circular/ANALYSIS  DNA,
       Viral/ANALYSIS  Human  HIV Envelope Protein gp120/CHEMISTRY
       HIV-1/*ISOLATION & PURIF/PATHOGENICITY  In Situ Hybridization/METHODS
       Macaca nemestrina/*VIROLOGY  Molecular Sequence Data  Monocytes/VIROLOGY
       Peptide Fragments/CHEMISTRY  Polymerase Chain Reaction/METHODS  RNA,
       Messenger/ANALYSIS  RNA, Viral/ANALYSIS  Support, U.S. Gov't, P.H.S.
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

