       Document 0524
 DOCN  M9620524
 TI    Dissociated expression of granulocyte-macrophage CSF and IL-3 in
       short-term T cell clones from normal mice.
 DT    9602
 AU    Fitzpatrick DR; Kelso A; Transplantation Biology Unit, Queensland
       Institute of Medical; Research, Australia.
 SO    J Immunol. 1995 Dec 1;155(11):5140-50. Unique Identifier : AIDSLINE
       MED/96072786
 AB    Granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-3 are
       generally thought to be produced in a coordinated fashion by all
       activated T cells. We have examined this premise using quantitative
       kinetic analyses of the expression of GM-CSF and IL-3 in clonal cultures
       of mouse T cells for the first two weeks following in vitro stimulation
       with solid-phase TCR- and accessory molecule-specific mAbs. We
       demonstrate that 1) differential secretion of GM-CSF and IL-3 is a
       feature of high proportions of CD8+ and CD4+ T cell clones, for extended
       periods of time following activation; 2) multiple patterns of expression
       of these two cytokines can occur, including equivalent, GM-CSF-biased,
       and IL-3-biased production, and a pattern that switches over a period of
       days from GM-CSF-biased to IL-3-biased production; 3) the disparate
       relative levels of secretion are not due to differential consumption of
       either cytokine; 4) altered T cell activation by addition of CD28
       costimulation accelerates both GM-CSF and IL-3 production but biased
       patterns of expression are retained, and 5) most T cells are not
       pre-committed to a particular pattern of relative GM-CSF and IL-3
       expression; instead, the potential for multiple patterns may persist for
       several days following in vitro activation. The results indicate that T
       cells have the potential to display previously unrecognized diversity of
       expression of GM-CSF and IL-3.
 DE    Animal  Antigens, CD28/PHYSIOLOGY  Clone Cells  CD4-Positive
       T-Lymphocytes/METABOLISM  CD8-Positive T-Lymphocytes/METABOLISM  Female
       Granulocyte-Macrophage Colony-Stimulating Factor/*BIOSYNTHESIS/
       METABOLISM  Interleukin-3/*BIOSYNTHESIS/METABOLISM  Mice  Mice, Inbred
       BALB C  Signal Transduction  Support, Non-U.S. Gov't  T-Lymphocyte
       Subsets/*METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

