       Document 0627
 DOCN  M9620627
 TI    Aberrant activation of CD8+ T-cell and CD8+ T-cell subsets in patients
       with newly diagnosed IDDM.
 DT    9602
 AU    Hehmke B; Michaelis D; Gens E; Laube F; Kohnert KD; Department of
       Experimental and Clinical Endocrinology, University; of Greifswald,
       Karlsburg, Germany.
 SO    Diabetes. 1995 Dec;44(12):1414-9. Unique Identifier : AIDSLINE
       MED/96082212
 AB    Two- and three-color cytofluorimetric techniques were used to study the
       expression patterns of the activation antigen HLA-DR on peripheral blood
       immunoregulatory T-cells from 25 patients with newly diagnosed
       insulin-dependent diabetes mellitus (IDDM) and 14 age- and sex-matched
       control subjects. The mean percentage of total activated (CD3+HLA-DR+)
       T-cells was significantly elevated in the IDDM group compared with the
       control group (P < 0.001). In control subjects, basal activation of CD4+
       and CD8+ lymphocytes accounted for the low percentage levels of
       activated T-cells. In contrast, the majority of IDDM patients showed an
       unbalanced activation of CD4+ and CD8+ lymphocytes with predominant
       activation of the CD8+ lymphocyte subset. The composition of the
       activated T-cell fraction was dependent on the composition of the total
       (activated + nonactivated) T-cell population, as indicated by the
       positive correlation between the CD4+/CD8+ T-cell ratios in these two
       cell populations (r = 0.714; P < 0.001). Excessive activation of CD8+
       T-cells was attributable to similar increases in the proportions of CD8+
       CD45RA+HLA-DR+ (naive) and CD8+CD45RA-HLA-DR+ (memory) cells. Analysis
       of the CD11b-defined subsets revealed predominant activation of CD8+
       CD11b- (cytotoxic) T-cells; CD8+ CD16+ HLA-DR+ natural killer cells were
       unchanged. The distribution of HLA-DR+ cells among subsets of CD4+
       T-cells differed from the pattern in the CD8+ population in that
       selective activation of CD4+ CD45RA- (memory, helper-inducer) cells
       accounted for the small increase in activated CD4+ cells.(ABSTRACT
       TRUNCATED AT 250 WORDS)
 DE    Adolescence  Adult  Antigens, CD3/ANALYSIS  Autoantibodies/BLOOD  Child
       CD4-Positive T-Lymphocytes/IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  Diabetes Mellitus,
       Insulin-Dependent/*IMMUNOLOGY  Female  Flow Cytometry  Human  HLA-DR
       Antigens/ANALYSIS  Islets of Langerhans/IMMUNOLOGY  Killer Cells,
       Natural/IMMUNOLOGY  *Lymphocyte Transformation  Male  Support, Non-U.S.
       Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

