       Document 0483
 DOCN  M9620483
 TI    [Study on reverse transcriptase inhibiting antibody in plasma of HIV-1
       seropositive subjects]
 DT    9602
 AU    Saito T; Suzuki K; Ito A; Hayashi T; Watanabe S; Kondo M; Imai M;
       Department of Virology, Kanagawa Prefectural Public Health;
       Laboratories.
 SO    Kansenshogaku Zasshi. 1995 Aug;69(8):851-7. Unique Identifier : AIDSLINE
       MED/96083295
 AB    Reverse transcriptase (RT) inhibiting antibody in a series of plasma of
       HIV-1-seropositive subjects was quantitatively measured by poly A-linked
       colorimetric microtiter plate assay. The plasma were obtained from 6
       asymptomatic carrier (AC)s and from 3 patients who progressed to AIDS.
       They had been followed 29-51 months. RT inhibiting antibody levels in
       the plasma were measured by inhibition assay against HTLV-IIIB RT
       activity. In five of the 6 AC cases, RT inhibiting antibodies in the
       serial plasma maintained high levels, and 50% inhibiting titers of the
       serial plasma did not decrease throughout the observation periods (45-51
       months). HIV isolation from peripheral blood mononuclear cell (PBMC) of
       these 5 ACs did not succeed, and HIV p24 antigens were not detected in
       the plasma. In one AC case (046) RT inhibiting antibody levels gradually
       decreased after 48 months. In this case, HIV p24 antigen was not
       detected in the serial plasma throughout the observation period (48
       months), but HIV was isolated from PBMC after 27 months. On the other
       hand, RT inhibiting antibody levels in the serial plasma of all 3
       patients who progressed to AIDS gradually decreased in observation
       periods (29-35 months). HIV strains were isolated from these 3 cases.
       These results suggest that reduction of RT inhibiting antibody levels
       correlate well with the success of HIV isolation and with progression of
       clinical manifestation.
 DE    Acquired Immunodeficiency Syndrome/*ENZYMOLOGY/TRANSMISSION
       Antibodies/*BLOOD  AIDS Serodiagnosis  Biological Markers/BLOOD
       Colorimetry  Disease Progression  English Abstract  Follow-Up Studies
       Human  HIV Seropositivity/*ENZYMOLOGY/TRANSMISSION  *HIV-1/ISOLATION &
       PURIF  Reverse Transcriptase Inhibitors/*IMMUNOLOGY  Time Factors
       CLINICAL TRIAL  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

