       Document 0399
 DOCN  M9620399
 TI    Human immunodeficiency virus type 1 Vpr arrests the cell cycle in G2 by
       inhibiting the activation of p34cdc2-cyclin B.
 DT    9602
 AU    Re F; Braaten D; Franke EK; Luban J; Department of Microbiology,
       Columbia University College of; Physicians and Surgeons, New York, New
       YOrk 10032, USA.
 SO    J Virol. 1995 Nov;69(11):6859-64. Unique Identifier : AIDSLINE
       MED/96013783
 AB    Human immunodeficiency virus type 1 (HIV-1) vpr inhibits the replication
       of tumor cell lines and peripheral blood mononuclear cells. Here it is
       demonstrated that expression of vpr, either in the context of a provirus
       or from an independent genetic element, induces a discrete cell cycle
       arrest, with cells containing 4N DNA. Low cyclin B-associated kinase
       activity, as well as the status of p34cdc2 and cdc25C phosphorylation,
       indicates that the cascade of reactions which drives the cell into
       mitosis has not been initiated. The phosphatase inhibitor okadaic acid
       releases the block, suggesting that Vpr perturbs upstream regulatorsof
       the G2-M transition. These studies demonstrate that HIV-1 vpr has
       profound effects on the cellular factors which control entry into
       mitosis and indicate vpr's potential contribution to the cellular
       pathology associated with HIV-1 infection.
 DE    Animal  Base Sequence  *Cell Cycle  Cell Division  Comparative Study
       Cyclins/*METABOLISM  DNA Primers  Flow Cytometry  Gene Products,
       env/BIOSYNTHESIS  Gene Products, vpr/*METABOLISM  Genes, vpr  G2 Phase
       Hela Cells  Human  HIV Core Protein p24/BIOSYNTHESIS
       HIV-1/GENETICS/*PHYSIOLOGY  Mammals  Molecular Sequence Data  Polymerase
       Chain Reaction  Protein p34cdc2/*ANTAGONISTS & INHIB/METABOLISM
       Support, Non-U.S. Gov't  Transfection  Viral Regulatory
       Proteins/BIOSYNTHESIS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

