       Document 0477
 DOCN  M9620477
 TI    Experimental hypersensitivity pneumonitis: in vitro effects of
       interleukin-2 and interferon-gamma.
 DT    9602
 AU    Fei R; Gott K; Edwards B; Schuyler M; Department of Medicine,
       Albuquerque Veterans Administration; Medical Center, NM 87108, USA.
 SO    J Lab Clin Med. 1995 Nov;126(5):485-94. Unique Identifier : AIDSLINE
       MED/96074348
 AB    Cultured CD4+ cells are responsible for transfer of adoptive murine
       experimental hypersensitivity pneumonitis (EHP) (ARRD 1992; 146:1582-8).
       To characterize interactions that occur in vitro that result in cells
       able to transfer EHP, we added either antibody to IFN-gamma, antibody to
       IL-2, or 30 or 300 micrograms/ml IFN-gamma at the onset of 72-hour
       culture of C3H/HeJ spleen cells from either M. faeni or ovalbumin
       (control) sensitized donors with 30 micrograms/ml Micropolyspora faeni.
       We determined the phenotype of cells after culture and the amount of
       IL-2 or IFN-gamma in the culture supernatants, transferred cells to
       naive recipients, challenged the recipients intratracheally with M.
       faeni, and determined the extent of pulmonary inflammatory changes 4
       days thereafter. Substantial amounts of IL-2 and IFN-gamma were detected
       in supernatants of cultures from M. faeni-sensitized animals, and lesser
       amounts were detected in culture supernatants from ovalbumin-sensitized
       donors. Treatment of cultures of M. faeni-sensitized cells with antibody
       to IL-2 or IFN-gamma blocked or reduced measurable IL-2 or IFN-gamma for
       the duration of culture. Treatment with IFN-gamma blocked increased
       levels of IL-2 at 48 and 72 hours of culture. Cultured M.
       faeni-sensitized cells adoptively transfer EHP. Cells from cultures
       depleted of either IL-2 or IFN-gamma or supplemented with IFN-gamma
       could transfer EHP equally well. We conclude that in vitro maturation of
       cells capable of adoptive EHP is not dependent on soluble IL-2 or
       IFN-gamma and is not altered by exogenous IFN-gamma.
 DE    Alveolitis, Extrinsic Allergic/*IMMUNOLOGY/PATHOLOGY  Animal
       Antibodies, Monoclonal/PHARMACOLOGY  Antigens, Bacterial/IMMUNOLOGY
       Cytokines/BIOSYNTHESIS/IMMUNOLOGY  Immunophenotyping  Immunotherapy,
       Adoptive  Interferon Type II/IMMUNOLOGY/*PHARMACOLOGY
       Interleukin-2/IMMUNOLOGY/*PHARMACOLOGY  Lung/PATHOLOGY  Male  Mice
       Mice, Inbred BALB C  Mice, Inbred C3H  Micromonosporaceae/IMMUNOLOGY
       Ovalbumin/IMMUNOLOGY  Spleen/CYTOLOGY/DRUG EFFECTS  Support, U.S. Gov't,
       Non-P.H.S.  Support, U.S. Gov't, P.H.S.  Th1 Cells/IMMUNOLOGY  Th2
       Cells/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

