       Document 0987
 DOCN  M9620987
 TI    A simple and rapid flow cytometric method to measure lymphocyte
       activation in HIV+ subjects. Diminished response to pokeweed mitogen in
       early disease.
 DT    9602
 AU    Jagers E; De Brabander M; Baisier A; De Cree J; Verhaegen H; Verbiest W;
       Stoffels P; Clinical Research Unit St. Bartholomeus, Jan Palfijn
       Hospital,; Merksem, Belgium.
 SO    Anal Cell Pathol. 1995 Jun;8(4):345-55. Unique Identifier : AIDSLINE
       MED/96082377
 AB    It was our objective to investigate the effect of asymptomatic infection
       with HIV on the expression of lymphocyte activation markers after
       stimulation with mitogens. Whole blood cultures were made of HIV+ and
       HIV- subjects (29 asymptomatic HIV-1-infected subjects and 33 apparently
       healthy normal volunteers). At various times after stimulation with
       concanavalin A (Con A), anti-CD3 and pokeweed mitogen (PWM), the
       expression of activation markers (CD25 and HLA-DR) and the blastogenesis
       were quantified by flow cytometry. The flow cytometric quantification of
       the expression of activation markers and blastogenesis in whole blood
       cultures provided an easy and safe alternative to thymidine
       incorporation to assess lymphocyte responses in HIV+ subjects.
       Activation showed a tendency to be lower in the HIV+ subjects with all
       three stimulants. This difference with HIV- subjects was statistically
       significant only for stimulation with PWM after 4 days. Further
       investigations should be undertaken to show whether this functional
       impairment is related to disease progression and whether it can be
       influenced by effective therapy.
 DE    Adult  Antigens, Differentiation/ANALYSIS  Biological Markers/ANALYSIS
       Dose-Response Relationship, Immunologic  Female  Flow Cytometry  Human
       HIV Infections/*IMMUNOLOGY  Immunophenotyping  Lymphocyte
       Transformation/*DRUG EFFECTS  Male  Middle Age  Pokeweed
       Mitogens/*PHARMACOLOGY  Time Factors  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

