       Document 1072
 DOCN  M9621072
 TI    The evolving molecular epidemiology of HIV-1 envelope subtypes in
       injecting drug users in Bangkok, Thailand: implications for HIV vaccine
       trials.
 DT    9602
 AU    Kalish ML; Baldwin A; Raktham S; Wasi C; Luo CC; Schochetman G; Mastro
       TD; Young N; Vanichseni S; Rubsamen-Waigmann H; et al; Division of
       HIV/AIDS, Centers for Disease Control and Prevention,; Atlanta, Georgia
       30333, USA.
 SO    AIDS. 1995 Aug;9(8):851-7. Unique Identifier : AIDSLINE MED/96014957
 AB    OBJECTIVE: To genetically characterize HIV-1 strains in injecting drug
       users (IDU) in Bangkok, Thailand in 1994, and compare these with strains
       found earlier in Thai IDU; such information is essential for HIV-1
       vaccine development and evaluation. METHODS: Peripheral blood
       mononuclear cells were collected from 84 IDU attending 14 drug treatment
       clinics in Bangkok in 1994. DNA was amplified using a nested polymerase
       chain reaction (PCR) procedure and sequenced directly (without cloning)
       from the PCR products. The V3 and flanking regions (345 nucleotides) of
       the env gene were analyzed using a neighbor-joining tree. RESULTS: Only
       one (1%) strain was a typical subtype B virus, 69 (82%) were genetically
       distinct subtype B' viruses (Thai B), and 14 (17%) were subtype E
       strains (Thai A). Persons with recently acquired infection were more
       likely to have subtype E viruses (P < 0.001) than those in our 1991
       survey, who were more likely to have subtype B' viruses. Pairwise
       intra-subtype differences within subtypes E and B' were 5.3 and 4.3%,
       respectively, compared with 3.4 and 3.5% among strains collected in 1991
       in Thailand. CONCLUSION: The genetic diversity within subtypes B' and E
       in Thailand and the proportion of new infections due to subtype E
       viruses among Bangkok IDU are increasing significantly. These data
       highlight the importance of monitoring the molecular epidemiology of
       HIV-1 in populations being considered for HIV-1 vaccine trials.
 DE    Amino Acid Sequence  AIDS Vaccines/PHARMACOLOGY  Base Sequence  Clinical
       Trials  DNA Primers/GENETICS  DNA, Viral/GENETICS  Epidemiology,
       Molecular  Female  *Genes, env  Glycosylation  Human  HIV Envelope
       Protein gp120/CHEMISTRY/GENETICS  HIV
       Infections/*COMPLICATIONS/EPIDEMIOLOGY/*VIROLOGY
       HIV-1/*CLASSIFICATION/*GENETICS/ISOLATION & PURIF  Male  Molecular
       Sequence Data  Phylogeny  Sequence Homology, Amino Acid  Substance
       Abuse, Intravenous/*COMPLICATIONS/*VIROLOGY  Thailand/EPIDEMIOLOGY
       Variation (Genetics)  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

