       Document 0197
 DOCN  M9620197
 TI    Broadly neutralizing, MN-like PND-directed antibodies in Rwandan
       children with long-term HIV1 infection.
 DT    9602
 AU    Fraisier C; Van de Perre P; Lepage P; Hitimana DG; Karita E; Desgranges
       C; INSERM U271, Lyon, France.
 SO    Res Virol. 1995 May-Jun;146(3):201-10. Unique Identifier : AIDSLINE
       MED/96075770
 AB    Sera from 11 perinatally HIV1-infected Rwandan children with prolonged
       survival were tested in vitro for the presence of neutralizing
       antibodies against different HIV1 strains. These 11 sera from long
       survivor (LS) children were compared with 16 sera from Rwandan children
       with AIDS. Sera from HIV1-infected children exhibited the greatest
       neutralizing activity against HIV1MN cell-free infection. They also
       inhibited HIV1RII and HIV1LAI cell-free infection with lower titres.
       Higher neutralization titres were observed in sera from LS compared to
       the AIDS group, with a significant difference for HIV1MN and HIV1LAI
       strains. Sera from LS children also inhibited syncytium formation
       induced by HIV1MN-infected cells with higher titres than AIDS children.
       Sera from the HIV1-infected children showed reactivity to the HIV1MN V3
       peptide, as well as to both the US/European and the African consensus V3
       peptides. Higher reactivity was observed in sera from LS than from AIDS
       children, and the difference was significant toward the African
       consensus peptide. The LS children also had significantly higher V3MN
       IgG avidity than the AIDS children. These data support the notion that
       the humoral response to the V3 domain, associated with a broadly
       neutralizing activity, may be an important factor in the prolonged
       survival of these children. The specificity against HIV1MN also suggests
       that an antigenically MN-related strain may be prevalent in Rwanda, and
       that an MN-related principal neutralizing domain sequence could be an
       important determinant for candidate vaccines in this part of Africa.
 DE    Amino Acid Sequence  Cell Line  Child  Child, Preschool  Cytopathogenic
       Effect, Viral  Female  Giant Cells/VIROLOGY  Human  HIV
       Antibodies/*BLOOD/IMMUNOLOGY  HIV Envelope Protein gp120/IMMUNOLOGY  HIV
       Infections/*IMMUNOLOGY  HIV-1/*IMMUNOLOGY  IgG/IMMUNOLOGY  Infant
       Molecular Sequence Data  Neutralization Tests  Peptide
       Fragments/IMMUNOLOGY  Rwanda  Support, Non-U.S. Gov't  Survivors
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

