       Document 0767
 DOCN  M9620767
 TI    Neutralization of HIV-1 by secretory IgA induced by oral immunization
       with a new macromolecular multicomponent peptide vaccine candidate.
 DT    9602
 AU    Bukawa H; Sekigawa K; Hamajima K; Fukushima J; Yamada Y; Kiyono H; Okuda
       K; Department of Oral and Maxillofacial Surgery, Yokohama City;
       University School of Medicine, Japan.
 SO    Nat Med. 1995 Jul;1(7):681-5. Unique Identifier : AIDSLINE MED/96071531
 AB    Control of pandemic infection of human immunodeficiency virus type 1
       (HIV-1) requires some means of developing mucosal immunity against HIV-1
       because sexual transmission of the virus occurs mainly through the
       mucosal tissues. However, there is no evidence as yet that the secretory
       immunoglobulin A (IgA) antibody induced by immunization with antigens in
       experimental animals can neutralize HIV-1. We demonstrate here that oral
       immunization with a new macromolecular peptide antigen and cholera toxin
       (CT) induces a high titre (1:2) of gut-associated and secretory IgA
       antibody to HIV-1. Using three different neutralizing assays, we clearly
       demonstrate that this secretory IgA antibody is able to neutralize
       HIV-1IIIB, HIV-1SF2 and HIV-1MN. Our new approach may prove to be
       important in the development of a mucosal vaccine that will provide
       protection of mucosal surfaces against HIV-1.
 DE    Administration, Oral  Amino Acid Sequence  Animal  Antibody Specificity
       Antigens, CD4/METABOLISM  AIDS Vaccines/ADMINISTRATION &
       DOSAGE/*IMMUNOLOGY  Binding Sites  Cholera Toxin/*IMMUNOLOGY  Consensus
       Sequence  Gastric Mucosa/IMMUNOLOGY  HIV
       Antibodies/BIOSYNTHESIS/*IMMUNOLOGY  HIV Envelope Protein
       gp120/*IMMUNOLOGY  HIV-1/*IMMUNOLOGY  IgA,
       Secretory/BIOSYNTHESIS/*IMMUNOLOGY  Intestinal Mucosa/IMMUNOLOGY  Japan
       Mice  Mice, Inbred BALB C  Molecular Sequence Data  Neutralization Tests
       Peptide Fragments/*IMMUNOLOGY  Support, Non-U.S. Gov't  Vaccines,
       Synthetic/ADMINISTRATION & DOSAGE/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

