       Document 0276
 DOCN  M9620276
 TI    Cloning of a new cytokine that induces IFN-gamma production by T cells.
 DT    9602
 AU    Okamura H; Tsutsi H; Komatsu T; Yutsudo M; Hakura A; Tanimoto T; Torigoe
       K; Okura T; Nukada Y; Hattori K; et al; Department of Bacteriology,
       Hyogo College of Medicine,; Nishinomiya, Japan.
 SO    Nature. 1995 Nov 2;378(6552):88-91. Unique Identifier : AIDSLINE
       MED/96061009
 AB    The mechanism underlying the differentiation of CD4+ T cells into
       functionally distinct subsets (Th1 and Th2) is incompletely understood,
       and hitherto unidentified cytokines may be required for the functional
       maturation of these cells. Here we report the cloning of a recently
       identified IFN-gamma-inducing factor (IGIF) that augments natural killer
       (NK) activity in spleen cells. The gene encodes a precursor protein of
       192 amino acids and a mature protein of 157 amino acids, which have no
       obvious similarities to any peptide in the databases. Messenger RNAs for
       IGIF and interleukin-12 (IL-12) are readily detected in Kupffer cells
       and activated macrophages. Recombinant IGIF induces IFN-gamma more
       potently than does IL-12, apparently through a separate pathway.
       Administration of anti-IGIF antibodies prevents liver damage in mice
       inoculated with Propionibacterium acnes and challenged with
       lipopolysaccharide, which induces toxic shock. IGIF may be involved in
       the development of Th1 cells and also in mechanisms of tissue injury in
       inflammatory reactions.
 DE    Amino Acid Sequence  Animal  Base Sequence  Cloning, Molecular
       Cytokines/*GENETICS/PHYSIOLOGY  DNA, Complementary  Gram-Positive
       Bacterial Infections/IMMUNOLOGY  Interferon Type II/*BIOSYNTHESIS
       Killer Cells, Natural/METABOLISM  Kupffer Cells/IMMUNOLOGY
       Lipopolysaccharides/IMMUNOLOGY  Liver Diseases/IMMUNOLOGY/PREVENTION &
       CONTROL  Male  Mice  Mice, Inbred BALB C  Molecular Sequence Data
       Propionibacterium acnes/IMMUNOLOGY  Recombinant Proteins
       Spleen/CYTOLOGY  T-Lymphocytes/*IMMUNOLOGY  Th1 Cells/IMMUNOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

