       Document 0299
 DOCN  M9620299
 TI    Ultrastructural study on experimental infection of rotavirus in a murine
       heterologous model.
 DT    9602
 AU    Majerowicz S; Kubelka CF; Stephens P; Barth OM; Departamento de
       Virologia, Instituto Oswaldo Cruz, Rio de; Janeiro, Brasil.
 SO    Mem Inst Oswaldo Cruz. 1994 Jul-Sep;89(3):395-402. Unique Identifier :
       AIDSLINE MED/96001440
 AB    Viral replication, histopathological and ultrastructural changes were
       observed for a period of nine days in the small intestine of suckling
       mice infected with a simian rotavirus (SA11). Samples taken from
       duodenum, jejunum and ileum were prepared for light microscopy,
       transmission and scanning electron microscopy analysis. Histopathologic
       effect could be detected within 8 hr post-infection, when only a few
       altered cells were observed. Damage was extensive after 16 hr
       post-infection, showing swollen enterocytes and reduced and irregularly
       oriented microvilli at intestinal villi tips. Virus particles were
       detected at 16 and 48 hr post-infection, budding from the viroplasm into
       the rough endoplasmic reticulum cisternae in ileum enterocytes. Clear
       evidence of viral replication, observed by electron microscopy was not
       described before in heterologous murine models. Regeneration of the
       intestinal villi began at the third day post-infection. Despite some
       differences observed in clinical symptoms and microscopic analysis of
       homologous and heterologous rotavirus infections, we concluded that
       mechanisms of heterologous rotavirus infection in mice follow similar
       patterns to those observed in the homologous models.
 DE    Animal  Intestines/PHYSIOPATHOLOGY/ULTRASTRUCTURE/*VIROLOGY  Mice
       Retroviruses, Simian/GROWTH & DEVELOPMENT/ISOLATION & PURIF/
       *ULTRASTRUCTURE  Rotavirus Infections/PATHOLOGY/*VIROLOGY  Virus
       Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

