       Document 0769
 DOCN  M9620769
 TI    Intracellular expression of antibody fragments directed against HIV
       reverse transcriptase prevents HIV infection in vitro [see comments]
 DT    9602
 AU    Maciejewski JP; Weichold FF; Young NS; Cara A; Zella D; Reitz MS Jr;
       Gallo RC; Hematology Branch, National Heart, Lung and Blood Institute,;
       National Institutes of Health, Bethesda, Maryland 20892-1652,; USA.
 SO    Nat Med. 1995 Jul;1(7):667-73. Unique Identifier : AIDSLINE MED/96071529
 CM    Comment in: Nat Med 1995 Jul;1(7):628-9
 AB    We have tested a novel strategy of intracellular immunization to block
       human immunodeficiency virus (HIV) infection. The expression of a
       specific antibody within a cell was achieved by transduction of genes
       that encode for immunoglobulin chains with specificity to viral reverse
       transcriptase. We demonstrated that inhibition of this enzyme makes
       cells resistant to HIV infection by blocking an early stage of viral
       replication. If high efficiency transduction with a stable vector into
       lymphohaematopoietic stem cells or mature lymphocytes can be achieved,
       gene transfer-mediated intracellular immunization might be a feasible
       treatment strategy in AIDS.
 DE    *Gene Therapy  Human  HIV Antibodies/*GENETICS/IMMUNOLOGY  HIV
       Infections/*PREVENTION & CONTROL
       HIV-1/*ENZYMOLOGY/IMMUNOLOGY/PHYSIOLOGY
       HIV-2/*ENZYMOLOGY/IMMUNOLOGY/PHYSIOLOGY  Immunoglobulins,
       Fab/*GENETICS/IMMUNOLOGY  Recombinant Fusion
       Proteins/BIOSYNTHESIS/IMMUNOLOGY  RNA-Directed DNA
       Polymerase/*IMMUNOLOGY  T-Lymphocytes/METABOLISM/*VIROLOGY  Transfection
       Tumor Cells, Cultured  Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

