       Document 1082
 DOCN  M9621082
 TI    Use of helper T cell-inducing peptides from conserved regions in HIV-1
       env in a noncovalent mixture with a CTL-inducing V3-loop peptide for in
       vivo induction of long-lasting systemic CTL response.
 DT    9602
 AU    Nehete PN; Arlinghaus RB; Sastry KJ; Department of Veterinary Sciences,
       University of Texas M.D.; Anderson Cancer Center, Bastrop 78602, USA.
 SO    Viral Immunol. 1994;7(4):189-97. Unique Identifier : AIDSLINE
       MED/96100713
 AB    Our previous reports established that immunization of mice in the
       footpad with a 15-amino acid synthetic peptide (R15K) from the V3 loop
       region in the envelope protein gp120 of human immunodeficiency virus
       type 1 (HIV-1) resulted in rapid induction of major histocompatability
       complex (MHC) class I-restricted, CD8+ HIV-1 envelope-specific cytotoxic
       T lymphocytes (CTLs) in the proximal popliteal lymph node. While
       efficient CTL activity could be assayed in lymph node cells for 8 to 10
       weeks after a single injection, spleen cells from these mice showed low
       to negligible levels of specific CTLs at 4 to 8 weeks postimmunization.
       We tested immunizing mice with a noncovalent mixture of a helper T cell
       (Th) activity-inducing peptide and R15K and observed efficient induction
       of R15K-specific CTL response that could be assayed up to 8 weeks
       postimmunization in cells obtained from both lymph node and spleen.
       Efficient CTL priming was observed when Th peptides from either of two
       different conserved regions in the HIV env were mixed with R15K,
       containing a dipalmityl-lysine-glycine-glycine moiety at the amino
       terminus. These data confirm reports in literature describing
       requirement of Th activity for efficient priming of CTL response in
       vivo. Additionally, these studies strongly suggest the possibility of
       formulating potential vaccine candidates consisting of mixtures of
       synthetic peptides capable of inducing Th and CTL responses in the
       context of multiple MHC haplotypes.
 DE    Amino Acid Sequence  Animal  Cytotoxicity, Immunologic  Hindlimb  Human
       HIV Envelope Protein gp120/ADMINISTRATION & DOSAGE/*BIOSYNTHESIS/
       IMMUNOLOGY  HIV-1/*IMMUNOLOGY  Immunization  Lymph Nodes/IMMUNOLOGY
       Mice  Mice, Inbred BALB C  Molecular Sequence Data  Peptide
       Fragments/ADMINISTRATION & DOSAGE/*BIOSYNTHESIS/  IMMUNOLOGY
       Spleen/IMMUNOLOGY  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       T-Lymphocytes, Cytotoxic/*IMMUNOLOGY/VIROLOGY  T-Lymphocytes,
       Helper-Inducer/*IMMUNOLOGY/VIROLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

