       Document 1021
 DOCN  M9621021
 TI    Multifaceted consequences of anti-gp41 monoclonal antibody 2F5 binding
       to HIV type 1 virions.
 DT    9602
 AU    Neurath AR; Strick N; Lin K; Jiang S; Lindsley F. Kimball Research
       Institute, New York Blood Center,; New York 10021, USA.
 SO    AIDS Res Hum Retroviruses. 1995 Jun;11(6):687-96. Unique Identifier :
       AIDSLINE MED/96078229
 AB    A human monoclonal antibody (MAb) (2F5) neutralizing a variety of
       laboratory strains and clinical isolates of the human immunodeficiency
       virus type 1 (HIV-1) and binding to an epitope of the envelope
       glycoprotein gp41 encompassing the amino acid sequence ELDKWA has been
       described (Muster T et al., J Virol 1993;67:6642-6647). It was suggested
       that an immunogen eliciting virus-neutralizing antibodies having a
       specificity similar to that of MAb 2F5 should be considered as a
       component of HIV-1 vaccines. Efforts in this direction would benefit
       from understanding the mechanism whereby MAb 2F5 neutralizes the
       infectivity of HIV-1. The segment of gp41 encompassing residues ELDKWA
       has so far not been directly implicated in initiation of infection by
       HIV-1, suggesting that MAb 2F5 might affect other sites on HIV-1
       envelope glycoproteins playing a role in virus entry into target cells.
       We provide here evidence that MAb 2F5 binding to HIV-1 virus particles
       decreases the accessibility or conformation of the gp41 fusion domain
       and of gp120 domains, including the binding site for the CD4 cell
       receptor. These apparently indirect consequences of MAb 2F5 binding to
       HIV-1 are likely to account for or contribute to the virus-neutralizing
       activity of this MAb.
 DE    Amino Acid Sequence  Antibodies, Monoclonal/*IMMUNOLOGY/METABOLISM
       Antigens, CD4/METABOLISM  Binding Sites  Cell Fusion  Epitope Mapping
       HIV Antibodies/*IMMUNOLOGY/METABOLISM  HIV Envelope Protein
       gp120/METABOLISM  HIV Envelope Protein
       gp41/CHEMISTRY/GENETICS/*IMMUNOLOGY  HIV-1/*IMMUNOLOGY  Molecular
       Sequence Data  Neutralization Tests  Peptide Fragments/CHEMICAL
       SYNTHESIS/IMMUNOLOGY/METABOLISM  Protein Conformation  Sequence
       Alignment  Support, U.S. Gov't, P.H.S.  Virion/*IMMUNOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

