       Document 0832
 DOCN  M9620832
 TI    Reversal of immunosuppression of lymphocyte proliferation caused by sera
       from persons with AIDS.
 DT    9602
 AU    Tomar RH; John P; Hinds P; Department of Pathology and Laboratory
       Medicine, University of; Wisconsin, Madison, USA.
 SO    Clin Diagn Lab Immunol. 1995 Jul;2(4):408-11. Unique Identifier :
       AIDSLINE MED/96082415
 AB    Sera from persons with AIDS contain inhibitors of lymphocyte
       proliferation. Inhibitory activity can be detected before the
       development of AIDS in humans. There appear to be at least three
       distinct suppressive moieties, one of which is prostaglandin E2. We and
       others had previously shown that serum samples from subjects with AIDS
       contained antibody to the cell line HUT 102B2. We attempted to remove
       this antibody and to determine if that action would also remove the
       inhibitory activity present in human immunodeficiency virus-positive
       sera. We incubated sera from subjects with AIDS with HUT 102B2 cells and
       tested the resultant supernatants for inhibition of cell proliferation.
       We found that this procedure significantly reversed inhibition by serum.
       Other cells and cell lines were similarly tested, but only HUT 102B2
       cells absorbed the inhibitory product(s). However, we determined that
       secretory material from another cell line, MLA 144, also reversed
       inhibition. The physical characteristics of the supernatant were
       investigated. Thus, two procedures and likely separate products,
       possibly cytokines, reverse immunosuppression by sera from persons with
       AIDS.
 DE    Absorption  Acquired Immunodeficiency Syndrome/*BLOOD/IMMUNOLOGY  Cell
       Line  Growth Inhibitors/*ANTAGONISTS & INHIB/BLOOD  Human  HIV
       Antibodies/ADVERSE EFFECTS/BLOOD/IMMUNOLOGY  Immunosuppressive
       Agents/*ANTAGONISTS & INHIB  *Lymphocyte Transformation
       Lymphokines/*ANTAGONISTS & INHIB/BLOOD  Support, Non-U.S. Gov't  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

