       Document 0744
 DOCN  M9620744
 TI    Methylcholanthrene-induced mouse sarcomas express individually distinct
       major histocompatibility complex class I-associated peptides recognized
       by specific CD8+ T-cell lines.
 DT    9602
 AU    Kono K; Petersson M; Ciupitu AM; Wen T; Klein G; Kiessling R;
       Microbiology and Tumor Biology Center, Karolinska Institute,; Stockholm,
       Sweden.
 SO    Cancer Res. 1995 Dec 1;55(23):5648-55. Unique Identifier : AIDSLINE
       MED/96075549
 AB    Mouse sarcomas induced by methylcholanthrene (MC) are immunologically
       distinct even if they are induced in the same strain of mice. T-cell
       lines were derived from mice immunized against a series of syngeneic MC
       sarcomas on B6 background, known to carry unique tumor-specific
       transplantation antigens. Tumor necrosis factor-alpha (TNF-alpha)
       release assays concurred with the in vivo rejection tests. The strongest
       response in the TNF-alpha release was always obtained with the
       corresponding tumor, with very limited cross-reactivity against five
       other MC tumors or two virally induced B6 lymphomas. The specific
       TNF-alpha release from the anti-MC tumor CTL lines was mainly mediated
       by CD8+ cells. T-cell lines from intact and CD4-/- mice gave a similarly
       specific pattern. In contrast, T-cell lines derived from CD8-/- mice
       cross-reacted with several other MC-induced tumors. Peptides eluted from
       MC sarcomas under mild acid conditions were fractionated by
       reverse-phase high performance liquid chromatography and tested for
       their ability to sensitize the processing- and presentation-defective
       mutant RMA-S line. Only one high performance liquid chromatographic
       fraction from each of the three different tumor-derived peptide eluates
       capacitated RMA-S to induce TNF-alpha release and sensitized the cell to
       the cytotoxic effect of the corresponding tumor-specific T-cell line. A
       different Kb-restricted peptide fraction was active for each of the
       three MC sarcomas tested, indicating that they all expressed
       individually distinct peptide epitopes.
 DE    Animal  Cross Reactions  CD8-Positive T-Lymphocytes/*IMMUNOLOGY
       Dose-Response Relationship, Immunologic
       Epitopes/IMMUNOLOGY/PHARMACOLOGY  Histocompatibility Antigens Class
       I/*METABOLISM/PHARMACOLOGY  Human  Methylcholanthrene  Mice  Mice,
       Inbred C57BL  Mice, Knockout  Peptide Fragments/IMMUNOLOGY  Sarcoma,
       Experimental/CHEMICALLY INDUCED/*IMMUNOLOGY  Support, Non-U.S. Gov't
       T-Lymphocytes, Cytotoxic/*IMMUNOLOGY  Tumor Cells, Cultured  Tumor
       Necrosis Factor/*SECRETION  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

