       Document 0845
 DOCN  M9620845
 TI    CD4+ and CD8+ lymphocytes of patients with AIDS synthesize increased
       amounts of interferon-gamma.
 DT    9602
 AU    Caruso A; Canaris AD; Licenziati S; Cantalamessa A; Folghera S; Lonati
       MA; de Panfilis G; Garotta G; Turano A; Institute of Microbiology,
       University of Brescia, Italy.
 SO    J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Dec 1;10(4):462-70.
       Unique Identifier : AIDSLINE MED/96074280
 AB    Individual cells capable of interferon-gamma (IFN-gamma) synthesis are
       easily detected by immunofluorescence and flow cytometric analysis using
       an anti-IFN-gamma monoclonal antibody as specific reagent. By IFN-gamma
       flow cytometry assay, we demonstrated that HIV-seropositive patients,
       starting at the early stage of viral infection, generally have an
       increased percentage of lymphocytes potentially able to produce
       IFN-gamma, compared with healthy blood donors. IFN-gamma expression in
       patient lymphocytes was observed to increase with the progressive stages
       of HIV infection, with the highest figures occurring in stage C
       patients. Such increased IFN-gamma expression involved both CD4+ and
       CD8+ T cell subsets. Most interestingly, we found patients at the same
       stage of HIV infection who had similar numbers of total and CD4+
       lymphocytes but highly different percentages of lymphocytes potentially
       capable of producing IFN-gamma.
 DE    Acquired Immunodeficiency Syndrome/COMPLICATIONS/*METABOLISM  Cells,
       Cultured  CD4-Positive T-Lymphocytes/DRUG EFFECTS/*METABOLISM
       CD8-Positive T-Lymphocytes/DRUG EFFECTS/*METABOLISM  Flow Cytometry
       Fluorescent Antibody Technique, Indirect  Human  HIV
       Seropositivity/COMPLICATIONS/METABOLISM  Immunophenotyping  Interferon
       Type II/*BIOSYNTHESIS  Ionomycin/PHARMACOLOGY  Ionophores/PHARMACOLOGY
       Lymphocyte Transformation/DRUG EFFECTS  Substance Abuse,
       Intravenous/COMPLICATIONS  Support, Non-U.S. Gov't  Tetradecanoylphorbol
       Acetate/PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

