       Document 0726
 DOCN  M9620726
 TI    CD4+/CD8+ ratio of liver-derived lymphocytes is related to viraemia and
       not to hepatitis C virus genotypes in chronic hepatitis C.
 DT    9602
 AU    Pham BN; Martinot-Peignoux M; Mosnier JF; Njapoum C; Marcellin P; Bougy
       F; Degott C; Erlinger S; Cohen JH; Degos F; Service d'Hematologie et
       Immunologie, Hopital Beaujon, Clichy,; France.
 SO    Clin Exp Immunol. 1995 Nov;102(2):320-7. Unique Identifier : AIDSLINE
       MED/96069882
 AB    The pathogenic mechanisms that lead to chronic hepatitis C are unknown.
       As hepatitis C virus (HCV) has been shown to induce T cell response, we
       assessed whether a particular T lymphocyte subset could be
       preferentially detected in the liver of patients with chronic hepatitis
       C in relation to viraemia or HCV genotypes. The immunophenotypes of
       liver-derived lymphocytes were analysed in 26 patients by flow cytometry
       and immunohistochemistry. Viraemia was quantified by branched DNA assay.
       Using this assay, HCV RNA was not detectable in six patients. HCV RNA
       was detected in 20 patients, and titres ranged from 8 to 137 x 10(6)
       Eq/ml. Genotyping was performed using a line probe assay. Type 1a, 1b,
       2a, 3a and 4a were found to infect 2, 10, 2, 7 and 3 patients,
       respectively. The CD4+/CD8+ ratio of liver-derived lymphocytes was
       significantly higher (P < 0.01) in patients with detectable viraemia
       than in patients without detectable viraemia. In contrast, neither the
       percentage of gamma/delta T lymphocytes nor that of CD2+CD57+ cells was
       different in the groups. When comparing the CD4+/CD8+ ratio, the
       percentage of gamma/delta T lymphocytes or CD2+CD57+ cells according to
       genotype, the differences were not significant. These results suggest
       that the CD4+/CD8+ ratio of liver-derived lymphocytes is related to
       viraemia but not to HCV genotypes in patients with chronic hepatitis C,
       and that T lymphocytes may be involved in the pathogenesis of liver
       lesions in chronic hepatitis C.
 DE    Adult  Chronic Disease  *CD4-CD8 Ratio  Female  Hepatitis C/*IMMUNOLOGY
       Hepatitis C Antibodies/IMMUNOLOGY  Hepatitis C Viruses/IMMUNOLOGY  Human
       Immunophenotyping  Liver/IMMUNOLOGY  Male  Middle Age  RNA, Viral/BLOOD
       Support, Non-U.S. Gov't  T-Lymphocyte Subsets/*IMMUNOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

