       Document 0889
 DOCN  M9620889
 TI    Detection and isolation of human T-cell leukemia/lymphoma virus type I
       (HTLV-I) from cultured lymphocytes of a Brazilian TSP/HAM patient.
 DT    9602
 AU    Andrada-Serpa MJ; Araujo AQ; Taffarel M; Schor D; Scheiner MA; Ferreira
       O; Schatzmayr HG; Secao de Imunologia, Instituto Nacional de Cancer, Rio
       de; Janeiro, RJ, Brasil.
 SO    Braz J Med Biol Res. 1995 Jan;28(1):51-7. Unique Identifier : AIDSLINE
       MED/96059252
 AB    Some Brazilian regions are considered to be endemic for human T-cell
       leukemia/lymphoma virus type I (HTLV-I) infection. Several studies have
       shown a high prevalence of HTLV-I infection among different groups such
       as blood donors, hemophiliacs and patients suffering from hematological
       and neurological diseases. Cases of adult T-cell leukemia/lymphoma as
       well as tropical spastic paraparesis/HTLV-I-associated myelopathy
       (TSP/HAM) have already been described in Brazil. We report the isolation
       of an HTLV-I strain from cultured lymphocytes of a TSP/HAM patient. An
       IL-2-dependent HTLV-I-infected T-cell line (ROB) expressing viral
       antigens was established and reverse transcriptase activity could be
       detected in the culture supernatant. Ultrastructural analysis showed
       immature and mature HTLV retrovirus particles. Finally, HTLV-I provirus
       type I was demonstrated by the polymerase chain reaction. This is the
       first isolation completely carried out in Latin America. The molecular
       analysis of viral strains, now in progress, should clarify the molecular
       epidemiology of HTLV-I in Brazil.
 DE    Adult  Base Sequence  Brazil/EPIDEMIOLOGY  Genome, Viral  Human
       HTLV-I/GENETICS/*ISOLATION & PURIF/ULTRASTRUCTURE  Lymphocytes/*VIROLOGY
       Male  Molecular Sequence Data  Paraparesis, Tropical
       Spastic/*EPIDEMIOLOGY/VIROLOGY  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

