       Document 0760
 DOCN  M9610760
 TI    Susceptibility of human monocytes to HIV type 1 infection in vitro is
       not dependent on their level of CD4 expression.
 DT    9601
 AU    Sonza S; Maerz A; Uren S; Violo A; Hunter S; Boyle W; Crowe S; AIDS
       Pathogenesis Research Unit, Macfarlane Burnet Centre for; Medical
       Research, Fairfield, Victoria, Australia.
 SO    AIDS Res Hum Retroviruses. 1995 Jul;11(7):769-76. Unique Identifier :
       AIDSLINE MED/96053838
 AB    Monocytes from HIV-seronegative persons were analyzed for CD4 expression
       and susceptibility to infection with HIV-1 on the day of isolation and
       following 1, 2, and 7 days in culture. Although surface CD4 was readily
       detected on freshly isolated monocytes, these cells were relatively
       resistant to infection. After 1 to 2 days in culture, when surface
       expression of CD4 had decreased over 90% to near background levels,
       cells became susceptible to infection with HIV-1. CD4 expression on
       monocytes cultured for 7 days was more than four times higher than that
       on freshly isolated cells, and the cultured cells were fully permissive
       to infection. These observations suggest that the differing
       susceptibility of monocytes and monocyte-derived macrophages to
       infection with HIV-1 is not simply proportional to the level of surface
       CD4 expression.
 DE    Antigens, CD/BIOSYNTHESIS/PHYSIOLOGY  Antigens,
       CD4/BIOSYNTHESIS/*PHYSIOLOGY  Cells, Cultured  Flow Cytometry  Human
       HIV Seronegativity/IMMUNOLOGY  HIV-1/*PHYSIOLOGY/PATHOGENICITY  Kinetics
       Lipopolysaccharides/PHARMACOLOGY  Lymphocytes/*IMMUNOLOGY/*VIROLOGY
       Macrophages/IMMUNOLOGY/VIROLOGY  Monocytes/DRUG
       EFFECTS/*IMMUNOLOGY/*VIROLOGY  Support, Non-U.S. Gov't  Time Factors
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

