       Document 0757
 DOCN  M9610757
 TI    Frequency of provirus-bearing CD4+ cells in HIV type 1 infection
       correlates with extent of in vitro apoptosis of CD8+ but not of CD4+
       cells.
 DT    9601
 AU    Carbonari M; Cibati M; Pesce AM; Sbarigia D; Grossi P; D'Offizi G; Luzi
       G; Fiorilli M; Department of Clinical Medicine, University of Rome La
       Sapienza,; Italy.
 SO    AIDS Res Hum Retroviruses. 1995 Jul;11(7):789-94. Unique Identifier :
       AIDSLINE MED/96053841
 AB    Lymphocytes from HIV-1-infected subjects undergo massive apoptosis when
       cultured in vitro, and this phenomenon might reflect pathogenetic
       mechanisms leading to immune dysfunction in vivo. However, (1)
       lymphocyte death is not restricted to CD4+ cells but seems to involve
       predominantly CD8+ cells, and (2) the same phenomenon occurs in other
       viral infections. Furthermore, it is not known whether a relationship
       exists between the HIV-1 burden and this type of cell death. In this
       work we sought to determine whether the HIV-1 provirus load correlates
       with the propensity to apoptosis of CD4+ and CD8+ cells. We studied 10
       HIV-1-infected patients with CD4+ cell counts above 500/mm3 and free of
       concomitant infections. We correlated the frequency of HIV-1-infected
       CD4+ cells with the extent of culture-induced apoptosis as well as with
       the phenotype of the apoptotic lymphocytes. We found that the magnitude
       of apoptosis correlated with the frequency of HIV-1-infected CD4+ cells
       (p = 0.0007), and that increasing viral load and apoptosis were
       associated with a shift to the selective death of CD8+ cells. Our data
       support the view that, in addition to CD4+ cell killing, another
       immunopathogenic effect of HIV might be that of priming CD8+ cells to
       apoptosis. In vivo, this could eventually lead to the exhaustion of the
       cytotoxic T cell compartment.
 DE    Adult  Apoptosis  Cell Survival  Cells, Cultured  Comparative Study
       CD4-Positive T-Lymphocytes/*IMMUNOLOGY/VIROLOGY  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY/VIROLOGY  Female  Flow Cytometry  Genome,
       Viral  Human  HIV Infections/*IMMUNOLOGY/VIROLOGY
       *HIV-1/GENETICS/ISOLATION & PURIF  HLA-DR Antigens/ANALYSIS
       Immunophenotyping  Lymphocytes/*IMMUNOLOGY/*VIROLOGY  Male  Middle Age
       Polymerase Chain Reaction/METHODS  Support, Non-U.S. Gov't  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

