       Document 0756
 DOCN  M9610756
 TI    Localization of human immunodeficiency virus Rev in transfected and
       virus-infected cells.
 DT    9601
 AU    Luznik L; Martone ME; Kraus G; Zhang Y; Xu Y; Ellisman MH; Wong-Staal F;
       Department of Medicine, UCSD School of Medicine, La Jolla,; California
       92093-0665, USA.
 SO    AIDS Res Hum Retroviruses. 1995 Jul;11(7):795-804. Unique Identifier :
       AIDSLINE MED/96053842
 AB    The rev gene product of human immunodeficiency virus (HIV) is obligatory
       for viral replication. Rev interacts specifically with a structured RNA
       sequence within the viral genome termed the REV response element (RRE).
       Although the importance of Rev for the expression of viral proteins is
       well documented, its functional mechanism remains unresolved. Previous
       studies identified Rev in the absence of RRE to be a nuclear protein
       localized primarily within the nucleoli. To extend our understanding of
       the role of Rev in viral replication, immunolocalization studies of Rev
       and other nuclear components were carried out in transfected cells
       expressing both the Rev protein and RRE-containing mRNA and in cells
       infected with HIV. In both types of cells, Rev-like immunoreactivity was
       distributed both in the nucleoplasm and cytoplasm. Within the nucleus,
       Rev immunoreactivity was not evenly distributed but was present within
       focal concentrations. In transfected cells that were double labeled for
       Rev and SC-35, which labels a known component of spliceosomes, the foci
       of Rev labeling were distinct from the speckles labeled by SC-35,
       although Rev foci and speckles were often juxtaposed. In addition,
       morphological changes in the three-dimensional network of speckles were
       observed in both transfected cells expressing both the Rev protein and
       RRE-containing mRNA and in cells infected with HIV-1 and HIV-2. Our
       observations are consistent with the proposed dual role of Rev in mRNA
       transport and splicing.
 DE    Base Sequence  Cell Nucleolus/VIROLOGY  DNA Primers  Gene Expression
       Gene Products, rev/*ANALYSIS/BIOSYNTHESIS  Genes, rev  Genome, Viral
       Hela Cells  Human  HIV-2/GENETICS/*PHYSIOLOGY  Molecular Sequence Data
       Polymerase Chain Reaction  Recombinant Proteins/ANALYSIS/BIOSYNTHESIS
       Restriction Mapping  RNA, Messenger/ANALYSIS/BIOSYNTHESIS  Support, U.S.
       Gov't, P.H.S.  Transfection  Viral Proteins/BIOSYNTHESIS  *Virus
       Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

