       Document 0752
 DOCN  M9610752
 TI    Human foamy virus DNA forms and expression in persistently infected Dami
       megakaryocytic cells.
 DT    9601
 AU    Wybier-Franqui J; Tobaly-Tapiero J; Coronel A; Giron ML; Chopin-Robert
       C; Peries J; Emanoil-Ravier R; UPR A0043, CNRS Retrovirus et
       Retrotransposons des Vertebres,; Hopital Saint-Louis, Paris, France.
 SO    AIDS Res Hum Retroviruses. 1995 Jul;11(7):829-36. Unique Identifier :
       AIDSLINE MED/96053846
 AB    We have characterized human foamy virus (HFV) proviral DNA and
       determined HFV expression in a persistent infection model, the Dami
       megakaryocytic cell line. Molecular studies were performed on parental
       persistently infected cells (Dami-P), as well as on derived clones
       (Dami-Cl). We report that in these nonlytic and non-HFV producer cells,
       viral DNA was found to be integrated into the cellular genome and that
       the few free proviral forms detected in Dami-P cells were deleted in
       their 5' LTR. Our molecular analysis indicates the presence of undeleted
       5' LTR forms in the integrated provirus within a proviral population
       mainly composed of deleted forms. In addition, the deletion in the bel1
       trans-activator gene, previously described by Saib et al., was found to
       be highly predominant. However, in 5-iodo-2'-deoxyuridine treated
       Dami-Cl cultures, virus production occurred, providing evidence for the
       presence of complete viral genome. Analysis of HFV expression in Dami-Cl
       cells, by Northern blot and immunoprecipitation, shows that the most
       striking difference between cytolytic and persistent HFV infection was
       the lack of expression of structural viral proteins, in contrast with
       Bet protein expression, which is maintained. Our data suggest that the
       Bet protein could be involved in the maintenance of viral persistency
       and that the persistently infected Dami system provides a suitable model
       for clarifying its function.
 DE    Blotting, Southern  Cell Line  Clone Cells  Comparative Study  DNA
       Probes  DNA, Viral/*BIOSYNTHESIS/CHEMISTRY/ISOLATION & PURIF  Human
       Leukemia, Megakaryocytic, Acute  Megakaryocytes
       Proviruses/GENETICS/PHYSIOLOGY  Restriction Mapping
       Spumavirus/GENETICS/*PHYSIOLOGY  Support, Non-U.S. Gov't  Viral
       Proteins/BIOSYNTHESIS/ISOLATION & PURIF  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

