       Document 0734
 DOCN  M9610734
 TI    Co-activation of naive CD4+ T cells and bone marrow-derived mast cells
       results in the development of Th2 cells.
 DT    9601
 AU    Huels C; Germann T; Goedert S; Hoehn P; Koelsch S; Hultner L; Palm N;
       Rude E; Schmitt E; Institut fur Immunologie, University of Mainz,
       Germany.
 SO    Int Immunol. 1995 Apr;7(4):525-32. Unique Identifier : AIDSLINE
       MED/96031348
 AB    Activation of naive dense CD4+ T cells by plate-bound anti-CD3
       antibodies favors the development of Th1 cells which, upon
       re-stimulation, produce significant amounts of IFN-gamma but no IL-4.
       However, co-activation of such naive T cells in the presence of IgE
       [anti-dinitrophenyl (DNP)]-loaded bone marrow-derived mast cells (BMMC)
       on plates coated with anti-CD3 antibodies and DNP-BSA led to the
       development of IL-4-producing Th2 cells. The same result could be
       observed if irradiated (800 rad) BMMC were applied as co-stimulators.
       Moreover, BMMC could be replaced by the supernatant of IgE-activated
       BMMC suggesting that a soluble mediator, presumably IL-4, was
       responsible for this effect. This assumption was substantiated using
       neutralizing anti-IL-4 antibodies which abolished the BMMC-mediated Th2
       development in all cases. Addition of IL-12, a cytokine that was shown
       to antagonize the Th2-promoting effect of IL-4 in vivo, could not
       inhibit the development of IL-4-producing T cells, but gave rise to a T
       cell population which produced relatively high amounts of IL-4 and
       IFN-gamma. Since BMMC represent the in vitro equivalent of mucosal mast
       cells these data suggest that IgE-activated mucosal mast cells can bias
       an emerging T cell dependent immune response towards a Th2 dominated
       reaction by the initial production of IL-4.
 DE    Animal  Antigens, CD3/IMMUNOLOGY  Bone Marrow/CYTOLOGY  Cells, Cultured
       Cytokines/BIOSYNTHESIS  CD4-Positive T-Lymphocytes/*PHYSIOLOGY  Female
       Interleukin-4/BIOSYNTHESIS/*PHYSIOLOGY  Ionomycin/PHARMACOLOGY
       *Lymphocyte Transformation  Male  Mast Cells/CYTOLOGY/*PHYSIOLOGY  Mice
       Mice, Inbred BALB C  Receptors, IgE/PHYSIOLOGY  Support, Non-U.S. Gov't
       Th2 Cells/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

