       Document 0730
 DOCN  M9610730
 TI    Sequence heterogeneity of murine acquired immunodeficiency syndrome
       virus: the role of endogenous virus.
 DT    9601
 AU    Gayama S; Vaupel BA; Kanagawa O; Department of Pathology and Medicine,
       Washington University; School of Medicine, St Louis, MO 63110, USA.
 SO    Int Immunol. 1995 May;7(5):861-8. Unique Identifier : AIDSLINE
       MED/96053548
 AB    A defective murine leukemia virus is the causative agent of murine
       acquired immunodeficiency syndrome (MAIDS). We have cloned cDNAs from
       both virus infected and non-infected cells using the PCR methods with
       primers corresponding to the franking sequence of the unique p12 gag
       gene. Sequence analysis of these cDNA clones revealed: (i) the presence
       of endogenous virus related to MAIDS virus in C57BL/6 mice, (ii) B cell
       lineage specific expression of endogenous virus and (iii) extensive
       heterogeneity of MAIDS virus recovered from virus infected cells due to
       the recombination of the related viruses (defective pathogenic virus,
       ecotropic virus and endogenous virus). These findings suggest that the
       creation of virus variants in infected cells may play an important role
       in virus pathogenesis and escape from immune attack during the
       development of MAIDS.
 DE    Animal  Base Sequence  Cell Line  Cell Transformation, Viral/GENETICS
       Gene Products, gag/BIOSYNTHESIS/*GENETICS  Leukemia Viruses,
       Murine/*GENETICS/ISOLATION & PURIF  Mice  Mice, Inbred C57BL  Molecular
       Sequence Data  Murine Acquired Immunodeficiency
       Syndrome/GENETICS/*VIROLOGY  Proviruses/*GENETICS/ISOLATION & PURIF
       RNA, Messenger/BIOSYNTHESIS  *Sequence Homology, Nucleic Acid  Support,
       U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

