       Document 0689
 DOCN  M9610689
 TI    Specific disulfide formation in the oxidation of HIV-1 zinc finger
       protein nucleocapsid p7.
 DT    9601
 AU    Yu X; Hathout Y; Fenselau C; Sowder RC 2nd; Henderson LE; Rice WG;
       Mendeleyev J; Kun E; Department of Chemistry and Biochemistry,
       University of Maryland; Baltimore County 21228-5398, USA.
 SO    Chem Res Toxicol. 1995 Jun;8(4):586-90. Unique Identifier : AIDSLINE
       MED/96015469
 AB    In vitro oxidation of the HIV-1 nucleocapsid protein p7 by the C-nitroso
       compound 3-nitrosobenzamide (NOBA) has been investigated. When
       reconstituted p7 was incubated with NOBA, three disulfide bonds were
       formed per molecule of p7, Cys 15-Cys 18, Cys 28-Cys 36, and Cys 39-Cys
       49. These were identified using the proteolytic enzyme endoproteinase
       Lys-C and mass spectrometry. When the denatured protein (Apo-p7) was
       incubated with NOBA, a more random pattern of multiple S-S linkages was
       found. Oxidation of reconstituted p7 also occurred on treatment with
       cupric ions (Cu2+), and the same three major disulfide bonds were formed
       as in the reaction with NOBA. These results suggest the interpretation
       that the oxidation reaction occurs at the zinc-binding centers while
       zinc cations are still bound and that the two zinc fingers are not
       identical in their chemical properties. This latter point is consistent
       with the independent biological roles reported previously for the two
       fingers in the viral infection cycle.
 DE    Amino Acid Sequence  Benzamides/CHEMISTRY  Capsid/*METABOLISM
       Copper/CHEMISTRY  Disulfides/*CHEMISTRY  Gene Products, gag/*METABOLISM
       Human  HIV-1/*METABOLISM  Molecular Sequence Data  Molecular Weight
       Nitroso Compounds/CHEMISTRY  NAD+ ADP-Ribosyltransferase/ANTAGONISTS &
       INHIB  Oxidation-Reduction  Protein Denaturation  Spectrum Analysis,
       Mass  Support, Non-U.S. Gov't  Support, U.S. Gov't, Non-P.H.S.  Support,
       U.S. Gov't, P.H.S.  *Zinc Fingers  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

