       Document 0683
 DOCN  M9610683
 TI    Preliminary evaluation of a Mycobacterium tuberculosis
       lipooligosaccharide (LOS) antigen in the serological diagnosis of
       tuberculosis in HIV seropositive and seronegative patients.
 DT    9601
 AU    Daleine G; Lagrange PH; Service de Microbiologie, Hopital Saint-Louis,
       Paris, France.
 SO    Tuber Lung Dis. 1995 Jun;76(3):234-9. Unique Identifier : AIDSLINE
       MED/96014892
 AB    OBJECTIVE: To assess the value of detection of specific IgG or IgM
       antibodies against three polar glycolipids of Mycobacterium tuberculosis
       in HIV seropositive and seronegative patients with documented active
       tuberculosis. DESIGN: Using an ELISA test, concentrations of IgM and IgG
       antibodies against phenolic glycolipid Tb1 (PGL Tb1), 2.3 diacyl
       trehalose (DAT) and polar lipooligosaccharide (LOS) were measured in the
       serum of 36 patients with active tuberculosis: 16 tuberculosis patients
       were coinfected with HIV (TB HIV+), and 20 were HIV seronegative (TB
       HIV-). 104 control sera were tested: they were collected from 50 healthy
       blood donors and from 54 asymptomatic HIV seropositive patients.
       Specificity, sensitivity and predictive positive and negative values
       were calculated. RESULTS: For the LOS antigen tested, sensitivity,
       specificity and predictive positive value of specific IgG antibodies
       were 75%, 95% and 84% respectively among the 36 tuberculosis patients.
       No significant results were obtained either with the IgM against the LOS
       antigen or with IgG against the DAT and PGL Tb1 antigens. Significant
       sensitivity and positive predictive values were demonstrated only with
       the ELISA test measuring specific IgG against the LOS antigen. The
       sensitivity was greater in the TB HIV+ subgroup (94%) as compared with
       the TB HIV- (60%) subgroup. These results were not related to different
       potential factors such as the site of disease (pulmonary or
       extrapulmonary) or by the acid-fast bacteria load as detected by the
       smear microscopic (Ziehl-Neelsen) examination. CONCLUSIONS: The results
       suggest that of the 3 antigens tested, only the LOS antigen is a
       potential marker for detecting the development of tuberculosis in HIV
       patients.
 DE    Antibodies, Bacterial/BLOOD  Antigens, Bacterial/*BLOOD/IMMUNOLOGY
       AIDS-Related Opportunistic Infections/*DIAGNOSIS  Biological
       Markers/ANALYSIS  Enzyme-Linked Immunosorbent Assay/METHODS  Human  HIV
       Seropositivity/*COMPLICATIONS  IgG/*BLOOD  IgM/*BLOOD
       Immunohistochemistry  Lipopolysaccharides/*BLOOD/IMMUNOLOGY
       Mycobacterium tuberculosis/IMMUNOLOGY  Tuberculosis/*DIAGNOSIS  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

