       Document 0676
 DOCN  M9610676
 TI    [Glycoprotein D (5-23) specific Th2-T-cell line induces HSV-1 keratitis]
 DT    9601
 AU    Heiligenhaus A; Jayaraman S; Soukiasian S; Dorf M; Foster CS; Hilles
       Immunology Laboratory, Massachusetts Eye and Ear; Infirmary, Harvard
       Medical School, Boston, USA.
 SO    Ophthalmologe. 1995 Aug;92(4):484-91. Unique Identifier : AIDSLINE
       MED/96056406
 AB    BALB/c inbred Igh-1-disparate mice exhibit different susceptibility to
       the development of HSV-1 stromal keratitis (HSK), which may be due to
       the differential immune regulation. CD4+ T lymphocytes may be critical
       for the disease induction. A T-cell line (CD4+, T-cell receptor V beta
       8+, interleukin-4+) specific for the N-terminal amino acids 5-23 of
       glycoprotein D from HSV-1 [gD(5-23)] was established from HSK
       susceptible C.AL-20 mice. HSK-resistant C.B-17 mice, and HSK-susceptible
       BALB/c mice were injected intraperitoneally with cells (5 x 10(5)/mouse)
       alone or combined with HSV-1 corneal inoculation (10(5) PFU, KOS
       strain). Control groups were injected with HSV-antigen-unrelated cells
       (PPD specific), or were only HSV-1 infected. Migration of the adoptively
       transferred gD(5-23) Th2 cells was analyzed by histology, by
       immunohistochemistry and by cell membrane labelling (PKH26). The
       transfer of gD(5-23) cells accelerated the disease onset (day 2,
       compared to day 7 without cells). The transfer of gD(5-23) cells
       increased the incidence of HSK (BALB/c 100%, C.B-17 20%) compared to
       mice that were only infected with HSV-1 (BALB/c 75%, C.B-17 0%).
       Keratitis was more severe in mice injected with gD(5-23) cells. In
       contrast, the transfer of PPD-specific cells did not influence the
       disease patterns. Mice injected with gD(5-23) cells and not inoculated
       with HSV-1 did not develop keratitis. The results suggest that CD4+ MHC
       class II, V beta 8+, IL-4 expressing T-cells (T helper 2) may be
       important for the induction of HSK.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Animal  Cell Line  Corneal Stroma/IMMUNOLOGY  English Abstract
       Herpesvirus 1, Human/*IMMUNOLOGY  Keratitis, Herpetic/*IMMUNOLOGY  Mice
       Mice, Inbred BALB C  Peptide Termination Factors/*IMMUNOLOGY  Support,
       Non-U.S. Gov't  Th2 Cells/*IMMUNOLOGY  Viral Envelope
       Proteins/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

