       Document 0666
 DOCN  M9610666
 TI    Alterations in cell-cell communication in human papillomavirus type 16
       (HPV16) transformed rat myoblasts.
 DT    9601
 AU    Ennaji MM; Schwartz JL; Mealing G; Belbaraka L; Parker C; Parentaux M;
       Jouishomme H; Arella M; Whitfield JF; Phipps J; National Research
       Council of Canada, Steacie Institute for; Molecular Sciences, Ottawa,
       Ontario.
 SO    Cell Mol Biol (Noisy-le-grand). 1995 Jun;41(4):481-98. Unique Identifier
       : AIDSLINE MED/96057185
 AB    A reduction of gap-junctional intercellular communication (GJIC) often
       accompanies neoplastic transformation. The present work demonstrates
       that transformation by the oncogenic human DNA virus, human papilloma
       virus 16(HPV16), also reduces GJIC between L6 rat myoblasts. HPVs are
       associated with anogenital cancers, the incidence of which is increasing
       in HIV positive patients of both sexes. Using videofluorescence imaging
       of Fura-2 loaded cells a lack of GJIC between transformed HPV16-L6 cells
       was first indicated by uncoordinated brief [Ca2+]i spikes in clusters of
       DMSO-treated HPV16-L6 cells instead of the synchronous, sustained
       [Ca2+]i surges in clusters of DMSO-treated L6 cells. Reduced GJIC
       between HPV16-L6 cells was demonstrated directly by a much reduced
       transfer of lucifer yellow dye from HPV16-L6 cells, which had been
       loaded with the dye through electroporation with an EPIZAP II in situ
       electroporator, to neighbouring nonelectroporated HPV16-L6 cells. One
       reason for this reduced GJIC between HPV16-L6 cells could have been
       their dramatically enhanced activity of membrane-associated PKC which is
       known to phosphorylate connexins and down-regulate gap junctions.
       However, the main reason was the viral-induced inhibition of the
       expression of a major gap junction component, Cx43 (Connexin 43), in the
       transformed myoblasts.
 DE    Animal  Calcium/METABOLISM  *Cell Communication  Cell Line, Transformed
       Cell Transformation, Viral  Connexin 43/BIOSYNTHESIS  Human
       Intercellular Junctions/METABOLISM/*VIROLOGY  Muscle,
       Skeletal/*CYTOLOGY/METABOLISM/VIROLOGY  *Papillomavirus, Human
       Phosphorylation  Protein Kinase C/METABOLISM  Rats  Support, Non-U.S.
       Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

