       Document 0655
 DOCN  M9610655
 TI    Simple, rapid and sensitive high-performance liquid chromatographic
       determination of delavirdine and its N-desisopropyl metabolite in human
       plasma.
 DT    9601
 AU    Staton BA; Johnson MG; Friis JM; Adams WJ; Biofluids Analytical
       Laboratory, Upjohn Co., Kalamazoo, MI 49001,; USA.
 SO    J Chromatogr B Biomed Appl. 1995 Jun 9;668(1):99-106. Unique Identifier
       : AIDSLINE MED/96035206
 AB    A method for the determination of a bisheteroarylpiperazine,
       non-nucleoside HIV-1 reverse transcriptase inhibitor, delavirdine, and
       its N-desisopropyl metabolite in human plasma, is described. Samples
       were deproteinized by addition of two parts of a solution of internal
       standard in acetonitrile (1 microgram/ml) to one part plasma. The
       supernatant was diluted with 10 mM phosphate buffer, pH 6.0, and
       injected onto the HPLC system. Fluorescence of the eluent was monitored
       with excitation at 302 nm and emission at 425 nm. Quantitation of
       delavirdine and its metabolite was achieved by comparing the peak-height
       ratio of each component relative to the internal standard to a
       through-the-origin linear regression curve determined from fortified
       plasma calibration standards. The assay was linear over the
       concentration range 0.02-17 microM for both delavirdine and its
       metabolite. The precision of the method, as expressed by the mean C.V.
       of the back-calculated, non-zero, standard concentrations, was +/- 4.4%
       for delavirdine and +/- 4.3% for the metabolite. The assay has been
       validated and utilized to analyze samples from human and animal
       pharmacokinetic studies.
 DE    Artifacts  Chromatography, High Pressure Liquid/*METHODS  Human
       HIV-1/ENZYMOLOGY  Indoles/*BLOOD  Piperazines/*BLOOD  Reference
       Standards  Reproducibility of Results  Reverse Transcriptase Inhibitors
       Spectrometry, Fluorescence  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

