       Document 0654
 DOCN  M9610654
 TI    Candida dubliniensis sp. nov.: phenotypic and molecular characterization
       of a novel species associated with oral candidosis in HIV-infected
       individuals.
 DT    9601
 AU    Sullivan DJ; Westerneng TJ; Haynes KA; Bennett DE; Coleman DC;
       University of Dublin, School of Dental Science, Department of; Oral
       Medicine and Pathology, Trinity College, Republic of; Ireland.
 SO    Microbiology. 1995 Jul;141 ( Pt 7):1507-21. Unique Identifier : AIDSLINE
       GENBANK/Z48346
 AB    Atypical oral Candida isolates were recovered from 60 HIV-infected and
       three HIV-negative individuals. These organisms were germ-tube-positive
       and produced abundant chlamydospores which were frequently arranged in
       triplets or in contiguous pairs. They belonged to C. albicans serotype A
       and had atypical carbohydrate assimilation profiles. Fingerprinting the
       genomic DNA of a selection of these organisms with the C.
       albicans-specific probe 27A and five separate oligonucleotides,
       homologous to eukaryotic microsatellite repeat sequences, demonstrated
       that they had a very distinct genomic organization compared to C.
       albicans and C. stellatoidea. This was further established by random
       amplified polymorphic DNA (RAPD) and karyotype analysis. Comparison of
       500 bp of the V3 variable region of the large ribosomal subunit genes
       from nine atypical isolates and the corresponding sequences determined
       from C. albicans, C. stellatoidea, C. tropicalis, C. parapsilosis, C.
       glabrata, C. kefyr and C. krusei showed that they atypical organisms
       formed a homogeneous cluster (100% similarity) that was significantly
       different from the other Candida species analysed, but was most closely
       related to C. albicans and C. stellatoidea. These genetic data combined
       with the phenotypic characteristics of these atypical organisms strongly
       suggest that they constitute a novel species within the genus Candida
       for which the name Candida dubliniensis is proposed.
 DE    Acquired Immunodeficiency Syndrome/*COMPLICATIONS  AIDS-Related
       Opportunistic Infections/*MICROBIOLOGY  Base Sequence
       Candida/CLASSIFICATION/*GENETICS/*PHYSIOLOGY  Candidiasis,
       Oral/COMPLICATIONS/*MICROBIOLOGY  Carbohydrates/METABOLISM  DNA
       Fingerprinting  DNA, Fungal/ANALYSIS  DNA, Ribosomal/GENETICS  Human
       Karyotyping  Molecular Sequence Data  Phenotype  Phylogeny  Polymerase
       Chain Reaction  Spores  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

