       Document 0629
 DOCN  M9610629
 TI    Positive correlation between the natural killer and gp 120/41-specific
       antibody-dependent cellular cytotoxic effector functions in HIV-infected
       individuals.
 DT    9601
 AU    Ahmad A; Menezes J; Laboratory of Immunovirology, University of
       Montreal, Quebec,; Canada.
 SO    J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Oct 1;10(2):115-9.
       Unique Identifier : AIDSLINE MED/96007272
 AB    Using our recently developed target system model of env gene-transfected
       cells for gp120/41-specific antibody-dependent cellular cytotoxicity
       (ADCC) assays, we evaluated the ADCC effector function of the peripheral
       blood mononuclear cells (PBMC) in 39 human immunodeficiency virus type 1
       (HIV-1)-infected individuals. The natural killer (NK) activity of the
       PBMC from these individuals against K562 was also determined. A
       significant positive correlation (p < or = 0.05, r = 0.37) was found
       between these two activities. On the basis of our data, we built a
       regression equation for the two activities whereby the ADCC effector
       function of PBMC of an HIV-infected individual can be predicted from
       their NK activity. We also found that the endpoint titer of a given
       serum for ADCC-mediating antibodies in the ADCC assay varied depending
       on the NK activity of the effector cells used: The effectors with high
       NK activity tended to give higher titers as compared with the effectors
       with low NK activity. To our knowledge, this is the first formal
       documentation of the existence of a positive correlation between the
       ADCC and NK activities of the PBMC. It also shows that the ADCC titer of
       serum in ADCC assay may vary depending on the NK activity of the
       effector cells used. This observation underscores an important variable
       of ADCC assays. Using our statistical model, one can predict the ADCC
       effector function of the PBMC from HIV-infected individuals from their
       NK activity and thus help clarify the role of this important immune
       response in HIV infection and AIDS prognosis.
 DE    Antibody-Dependent Cell Cytotoxicity/*IMMUNOLOGY  Cell Line  Cells,
       Cultured  Cytotoxicity, Immunologic/IMMUNOLOGY  Human  HIV
       Antibodies/IMMUNOLOGY  HIV Envelope Protein gp120/*IMMUNOLOGY  HIV
       Envelope Protein gp41/*IMMUNOLOGY  HIV Infections/*IMMUNOLOGY
       HIV-1/*IMMUNOLOGY  Killer Cells, Natural/*IMMUNOLOGY
       Lymphocytes/IMMUNOLOGY  Regression Analysis  Support, Non-U.S. Gov't
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

