       Document 0612
 DOCN  M9610612
 TI    Heterogeneity of spindle cells in Kaposi's sarcoma: comparison of cells
       in lesions and in culture.
 DT    9601
 AU    Kaaya EE; Parravicini C; Ordonez C; Gendelman R; Berti E; Gallo RC;
       Biberfeld P; Immunopathology Laboratory, Karolinska Institute,
       Stockholm,; Sweden.
 SO    J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Nov 1;10(3):295-305.
       Unique Identifier : AIDSLINE MED/96027797
 AB    The immunophenotype of spindle cells in epidemic, endemic, and classic
       (sporadic) Kaposi's sarcoma (KS) lesions was defined by the
       demonstration of various cell markers and compared with that of
       KS-derived cell lines. No significant histological or immunophenotypic
       differences were observed between the three clinical types of KS at
       comparable stages. The spindle-cell compartment of the different KS
       types was composed predominantly of a mixture of proliferating
       CD45+/CD68+ bone-marrow-derived monocytes and TE7+/collagen+
       fibroblastic cells with varying expression of EN4/PAL-E/CD31/CD34/CD36
       endothelial-associated antigens and/or smooth-muscle-specific
       alpha-actin (alpha-actin). The latter cells appeared to represent
       transitional forms of fibroendothelial and fibromyocytic cells. The in
       vitro cultured KS-derived cell lines (KS-3, KS-6, and KS-8) expressed
       the fibroblastic antigen TE7 and smooth-muscle-specific alpha-actin but
       not leukocytic or endothelial-associated antigens consistent with the
       phenotype of fibromyoid spindle cells of primary lesions. Neither HIV
       antigen nor provirus DNA was demonstrable in the epidemic KS lesions.
       The observed heterogeneity of the spindle-cell compartment further
       substantiates the view that Kaposi's sarcoma, irrespective of clinical
       setting, expresses salient features more compatible with reactive,
       tumor-like lesion than clonal sarcoma.
 DE    Acquired Immunodeficiency Syndrome/COMPLICATIONS  Antibodies, Monoclonal
       Antigens, CD/ANALYSIS  Antigens, Differentiation/ANALYSIS  Base Sequence
       Comparative Study  Cytoskeletal Proteins/ANALYSIS  DNA Primers/CHEMISTRY
       Endothelium, Vascular/CHEMISTRY/PATHOLOGY  Extracellular Matrix
       Proteins/ANALYSIS  Fibroblasts/CHEMISTRY/PATHOLOGY  Human  Immunoenzyme
       Techniques  Immunophenotyping  Leukocytes/CHEMISTRY/PATHOLOGY  Lymph
       Nodes/PATHOLOGY  Lymphatic Diseases/COMPLICATIONS  Molecular Sequence
       Data  Polymerase Chain Reaction  Sarcoma,
       Kaposi's/COMPLICATIONS/IMMUNOLOGY/*PATHOLOGY  Skin
       Neoplasms/COMPLICATIONS/IMMUNOLOGY/*PATHOLOGY  Support, Non-U.S. Gov't
       Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

