       Document 0589
 DOCN  M9610589
 TI    Sampling lymph node content of human immunodeficiency virus type 1
       nucleic acids and p24 antigen by fine-needle aspiration in early-stage
       patients.
 DT    9601
 AU    Anderson DW; DeNobile J; Zhao F; Vahey M; Lane J; Nau M; Brown AE;
       Wegner SA; Malone JD; Wagner K; et al; Division of Retrovirology, Walter
       Reed Army Institute of; Research, Washington, D.C., USA.
 SO    J Acquir Immune Defic Syndr Hum Retrovirol. 1995;10 Suppl 2:S57-61.
       Unique Identifier : AIDSLINE MED/96033813
 AB    The potential of lymph node fine-needle aspiration (LNFNA) for sampling
       viral load was evaluated in excised, peripheral lymph nodes from five
       patients with early-stage human immunodeficiency virus type 1 HIV-1
       disease (asymptomatic, CD4 cells > 300/mm3). The preponderance (> 80%)
       of viral RNA was within follicular germinal centers as noted by in situ
       hybridization on lymph node frozen sections (LNFSs) as well as within
       cohesive groups of 10-20 lymphoid cells (microfragments) in LNFNA
       preparations. Quantification of cells expressing HIV-1 RNA by in situ
       hybridization, quantification of HIV-1 gag RNA and gag DNA per 10(5)
       cells by polymerase chain reaction, and measurement of p24 antigen per
       10(5) cells yielded similar values for LNFNA, lymph node mononuclear
       cells (LNMCs) from tissue homogenates by Ficoll-Hypaque separation, and
       LNFS. Sampling of lymph node viral load by LNFNA appears to capture
       viral components associated with both individual expressing cells and
       follicular germinal centers. Due to the advantages in terms of patients'
       morbidity, repeatability, and cost, assessment of lymphoid tissue viral
       load by LNFNA warrants an in vivo feasibility trial as an alternative to
       lymph node biopsy.
 DE    Adult  Biopsy, Needle  DNA, Viral/*ANALYSIS  Female  Gene Products,
       gag/GENETICS  Human  Hyperplasia  HIV Core Protein p24/*ANALYSIS  HIV
       Infections/*VIROLOGY  HIV-1/GENETICS/IMMUNOLOGY/*ISOLATION & PURIF
       Immunohistochemistry  In Situ Hybridization  Lymph
       Nodes/CHEMISTRY/PATHOLOGY/*VIROLOGY  Male  Polymerase Chain Reaction
       RNA, Viral/*ANALYSIS  Support, Non-U.S. Gov't  Support, U.S. Gov't,
       Non-P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

