       Document 0558
 DOCN  M9610558
 TI    Protective effect of interferon-alpha against cell-mediated human
       immunodeficiency virus transmission resulting from coculture of infected
       lymphocytes with fetal trophoblasts.
 DT    9601
 AU    Bourinbaiar AS; Krasinski K; Borkowsky W; Lee-Huang S; Department of
       Biochemistry, New York University Medical Center,; NY 10016, USA.
 SO    J Interferon Cytokine Res. 1995 Jun;15(6):503-8. Unique Identifier :
       AIDSLINE MED/96006746
 AB    The hypothesis that the low transmission rate of HIV in utero may be
       due, in part, to the protective effect of IFN-producing placental
       trophoblasts was explored in vitro. The model consisted of H9
       lymphocytes, as surrogates of maternal HIV-infected T cells, incubated
       for 3 h with JEG-3 trophoblasts in the presence of 10-fold dilutions of
       leukocyte-derived IFN-alpha (from 1000 to 0.1 IU/ml). The dose effect
       was monitored either directly, by measuring the levels of proviral DNA
       by PCR after a single round of infection, or indirectly, by coculturing
       infected JEG-3 with cord blood-derived MT-4 lymphocytes and determining
       the levels of p24 production by ELISA. Both assays revealed a
       dose-dependent blocking effect of IFN-alpha on cell-mediated HIV
       transmission. The complete inhibition of HIV infection was observed in
       the presence of 100 IU IFN-alpha. The efficacy of such a low dose could
       not be attributed to insufficient viral load because up to 10(8)
       infectious particles could be transmitted during cell-cell contact. An
       adhesion assay ruled out the possibility that IFN-alpha acts through
       prevention of lymphocyte-trophoblast contact. The results suggest that
       physiologic levels of IFN-alpha, present in the placental environment,
       may contribute to the protection of the fetus against HIV infection.
 DE    Cell Adhesion/DRUG EFFECTS  Cell Communication/DRUG EFFECTS  Cells,
       Cultured  DNA, Viral/ANALYSIS  Female  Human  HIV Infections/PREVENTION
       & CONTROL/*TRANSMISSION  Interferon-alpha/*PHARMACOLOGY
       Lymphocytes/*DRUG EFFECTS/VIROLOGY  *Maternal-Fetal Exchange  Pregnancy
       Proviruses/GENETICS  Support, Non-U.S. Gov't  Support, U.S. Gov't,
       P.H.S.  Trophoblast/*DRUG EFFECTS/VIROLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

