       Document 0553
 DOCN  M9610553
 TI    HIV-plasma viral load detection by branched DNA signal amplification.
 DT    9601
 AU    Iuliano R; Forastieri G; Brizzi M; Mazzotta F; Ceccherini-Nelli L;
       Department of Biomedicine, University of Pisa.
 SO    New Microbiol. 1995 Jul;18(3):299-301. Unique Identifier : AIDSLINE
       MED/96028327
 AB    To detect HIV-1 plasma viral load in seropositive patients, we applied a
       new molecular quantitation technique: branched DNA signal amplification
       (bDNA). We performed bDNA with 99 sera from HIV-1 seropositive patients
       undergoing antiretroviral therapy. We compared the results obtained with
       p24 antigenemia and CD4+ cell count. The bDNA proved quite sensitive and
       available for routine use in laboratories.
 DE    Antibodies, Monoclonal/IMMUNOLOGY  Blood/*VIROLOGY  Chemiluminescence
       Comparative Study  CD4 Lymphocyte Count  DNA Probes/GENETICS
       Enzyme-Linked Immunosorbent Assay  Female  Human  HIV Core Protein
       p24/ANALYSIS  HIV Seropositivity/*BLOOD  HIV-1/IMMUNOLOGY/*ISOLATION &
       PURIF  Male  *Nucleic Acid Hybridization  RNA, Viral/*ISOLATION & PURIF
       Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

