       Document 0551
 DOCN  M9610551
 TI    Genetic variability in different Pneumocystis isolates from man and
       rats.
 DT    9601
 AU    Ortona E; Margutti P; De Luca A; Tamburrini E; Visconti E; Siracusano A;
       Department of Immunology, Istituto Superiore di Sanita, Roma,; Italy.
 SO    New Microbiol. 1995 Jul;18(3):335-40. Unique Identifier : AIDSLINE
       MED/96028335
 AB    To gain further insight into the genetic variability between different
       isolates of Pneumocystis carinii of rat and human origin, we sequenced
       three DNA regions from the mitochondrial rRNA gene, the 5S rRNA gene and
       the dihydrofolate reductase gene (DHFR) of microorganisms isolated from
       three different immunosuppressed rat sources and from one HIV + patient
       with P. carinii pneumonia. Some point mutations and deletions were found
       among rat isolates in two regions (mitochondrial rRNA gene and 5S rRNA
       gene). Nucleotide sequence variations in these conserved regions support
       the hypothesis of the existence of several Pneumocystis strains
       infecting the same host species.
 DE    Animal  Base Sequence  DNA Primers  DNA, Fungal/*GENETICS  DNA,
       Mitochondrial/*GENETICS  DNA, Ribosomal/*GENETICS  Human  Molecular
       Sequence Data  Pneumocystis carinii/*GENETICS  Polymerase Chain Reaction
       Rats  Rats, Sprague-Dawley  RNA, Ribosomal, 23S/GENETICS  RNA,
       Ribosomal, 5S/GENETICS  Sequence Alignment  Support, Non-U.S. Gov't
       Tetrahydrofolate Dehydrogenase/GENETICS  *Variation (Genetics)  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

