       Document 0539
 DOCN  M9610539
 TI    Evaluation of commercial HTLV-1 test kits by a standard HTLV-1 serum
       panel.
 DT    9601
 AU    Fujiyama C; Fujiyoshi T; Matsumoto D; Tamashiro H; Sonoda S; Department
       of Virology, Faculty of Medicine, Kagoshima; University, Japan.
 SO    Bull World Health Organ. 1995;73(4):515-21. Unique Identifier : AIDSLINE
       MED/96040929
 AB    To evaluate the performance of currently available test kits for human
       T-cell lymphotropic virus type 1 (HTLV-1), we examined two particle
       agglutination (PA) tests and nine enzyme immunoassays (EIA) using a
       standard serum panel consisting of HTLV-1-positive and HTLV-1-negative
       sera that had been characterized by immunofluorescence and the
       polymerase chain reaction (PCR). The PA kits exhibited 94.0-100.0%
       sensitivity and 99.5-100.0% specificity; the sensitivity range was
       ascribed to the quality of the HTLV-1 antigens coated on the particles.
       The EIA kits had 99.5-100% sensitivity and 98.5-100% specificity; the
       98.5%-99.5% specificity exhibited by five of the EIA kits could have
       been due to nonspecific reactions that were detected through use of an
       inadequate cut-off value and the use of recombinant proteins. It can be
       concluded that the sensitivity of the currently available PA and EIA
       kits is sufficient to permit their use for screening purposes; however,
       the specificity of some EIA kits should be optimized.
 DE    Agglutination Tests  Human  HTLV-I Infections/*DIAGNOSIS  Immunoenzyme
       Techniques  *Reagent Kits, Diagnostic/STANDARDS  Sensitivity and
       Specificity  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

