       Document 0533
 DOCN  M9610533
 TI    Expression of IL-10, IL-4 and interferon-gamma in unstimulated and
       mitogen-stimulated peripheral blood lymphocytes from HIV-seropositive
       patients.
 DT    9601
 AU    Diaz-Mitoma F; Kumar A; Karimi S; Kryworuchko M; Daftarian MP; Creery
       WD; Filion LG; Cameron W; Department of Paediatrics, Children's Hospital
       of Eastern; Ontario, University of Ottawa, Canada.
 SO    Clin Exp Immunol. 1995 Oct;102(1):31-9. Unique Identifier : AIDSLINE
       MED/96003947
 AB    Infection of immune cells with HIV induces dysregulation of cytokines
       which may play a vital role in HIV pathogenesis. We analysed the
       expression of T helper type 1 (Th1) (interferon-gamma (IFN-gamma)) and
       Th2 (IL-4, IL-10) type cytokines in peripheral blood lymphocytes (PBL)
       from HIV+ patients. The semiquantitative reverse transcriptase
       polymerase chain reaction (RT-PCR) analysis revealed that IFN-gamma mRNA
       in unstimulated PBL was significantly decreased and IL-10 mRNA was
       significantly upregulated in patients with < 400 CD4+ T cells/mm3 (n =
       30) as compared to patients with > 400 CD4+ T cells/mm3 (n = 6) and
       normal controls (n = 16). In addition, IL-10 mRNA levels were inversely
       associated with IFN-gamma expression. Similar results were obtained by
       measuring IL-10 production in the supernatants of PBL cultured in vitro
       without stimulation by employing an enzyme immunosorbent assay (ELISA).
       However, the levels of IL-4 and IFN-gamma produced by unstimulated PBL
       were undetectable by ELISA. Mitogen stimulation of PBL revealed two
       groups of HIV+ individuals based on IL-10 production. PBL from one set
       of individuals produced low levels of IL-10 (low IL-10 producers)
       whereas the other group produced IL-10 comparable to that of normal
       controls (IL-10 producers). Production of IL-4 was significantly reduced
       in HIV+ individuals with < 400 CD4+ T cells/mm3 as compared to the
       normal controls. However, ability to produce IFN-gamma by
       mitogen-stimulated total PBL and CD4+ purified cells was not impaired in
       HIV+ individuals. These results suggest that unstimulated and
       mitogen-stimulated PBL of HIV+ individuals exhibit dysregulation of Th2
       type cytokines which may play a role in HIV immunopathogenesis.
 DE    Adult  Base Sequence  Human  HIV Seropositivity/*IMMUNOLOGY  Interferon
       Type II/*BIOSYNTHESIS  Interleukin-10/*BIOSYNTHESIS
       Interleukin-4/*BIOSYNTHESIS  Lymphocyte Transformation  Molecular
       Sequence Data  Polymerase Chain Reaction  Support, Non-U.S. Gov't
       T-Lymphocytes, Helper-Inducer/*METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

