       Document 0532
 DOCN  M9610532
 TI    Demonstration of virus-specific CD8+ memory T cells in
       measles-seropositive individuals by in vitro peptide stimulation.
 DT    9601
 AU    Nanan R; Carstens C; Kreth HW; Universitats-Kinderklinik, Wurzburg,
       Germany.
 SO    Clin Exp Immunol. 1995 Oct;102(1):40-5. Unique Identifier : AIDSLINE
       MED/96003948
 AB    CD8+ cytotoxic T lymphocytes (CTL) in measles virus infection have been
       difficult to investigate due to the strong immunosuppressive effects
       exhibited by infectious measles virus in vitro. In order to circumvent
       immunosuppression we used predicted peptide epitopes to induce measles
       virus-specific CTL. This was done by screening the structural proteins
       of measles virus for HLA-A2.1 peptide-binding motifs with valine in
       position 2 and leucine in position 9. Synthetic peptides np210-218,
       np226-234, and np340-348 from the nucleoprotein, peptide hp29-37 from
       the haemagglutinin protein, and peptide pp 519-527 from the polymerase
       protein were synthesized and used to expand measles virus-specific CD8+
       CTL in vitro. Induction of CTL with synthetic peptides was restricted to
       HLA-A2-positive peripheral blood mononuclear cells (PBMC) from
       measles-seropositive individuals. We conclude that this method is a
       useful tool to demonstrate memory CD8+ CTL in measles-seropositive
       adults and to evaluate the role of structural proteins in CTL responses
       against measles.
 DE    Adult  Amino Acid Sequence  Cell Line  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  Human  HLA-A2 Antigen/ANALYSIS/IMMUNOLOGY
       *Immunologic Memory  Measles Virus/*IMMUNOLOGY  Molecular Sequence Data
       Peptide Fragments/*IMMUNOLOGY  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

