       Document 0529
 DOCN  M9610529
 TI    During HIV infection, CD4+ CD38+ T-cells are the predominant circulating
       CD4+ subset whose HLA-DR positivity increases with disease progression
       and whose V beta repertoire is similar to that of CD4+ CD38- T-cells.
 DT    9601
 AU    Ramzaoui S; Jouen-Beades F; Gilbert D; Borsa-Lebas F; Michel Y; Humbert
       G; Tron F; Groupe de Recherche en Immunopathologie, Institut Federatif
       de; Recherche Multidisciplinaire sur les Peptides, France.
 SO    Clin Immunol Immunopathol. 1995 Oct;77(1):33-41. Unique Identifier :
       AIDSLINE MED/96010051
 AB    Three-color automated flow cytometry was carried out on peripheral blood
       CD4+ and CD8+ T-lymphocytes of 42 HIV-positive patients using tri-color
       anti-CD4 or anti-CD8, phycoerythrin-anti-CD38, and
       fluorescein-anti-HLA-DR, mAbs to elucidate further the T-cell activation
       hypothesis recently proposed to explain CD4+ T-cell abnormalities
       observed during HIV infection. CD4+ CD38+ T-cells constituted the major
       part of circulating CD4+ T-cells in HIV-infected patients and their
       HLA-DR molecule positivity increased as their disease progressed. The
       level of CD38 and HLA-DR expression on CD4+ T-cells was positively
       correlated to that of CD8+ T-cells and to the level of beta
       2-microglobulin. Next, to determine whether CD38 expression was
       associated with a selective expansion or deletion of V beta gene-defined
       subsets, we compared the V beta gene frequencies between CD38+ and CD38-
       T-cells from HIV-infected CDC stage II patients using 13 mAbs specific
       to V beta families. While selective expansion of certain V beta families
       was observed in CD4+ and CD8+ T-cells the T-cell receptor V beta subset
       distribution was similar among CD38+ and CD38-, CD4+ and CD8+ T-cells,
       suggesting that CD38+ expression was either independent of an
       HIV-encoded antigen-driven process or rather indicative of T-cell
       immaturity. It is proposed that the phenotype of circulating CD4+ and
       CD8+ T-cells of HIV-infected patients is a feature of two different
       mechanisms: (i) an in vitro activation state responsible for increased
       DR expression and selective expansion of V beta gene-defined subsets,
       and (ii) T-cell immaturity due to an increased turnover of these cells
       and accounting for increased CD38 expression.
 DE    beta 2-Microglobulin/METABOLISM  Acquired Immunodeficiency
       Syndrome/*IMMUNOLOGY/PATHOLOGY  Adult  Antigens,
       Differentiation/*METABOLISM  Antiviral Agents/THERAPEUTIC USE  CD4
       Lymphocyte Count  CD4-CD8 Ratio  CD4-Positive T-Lymphocytes/*IMMUNOLOGY
       CD8-Positive T-Lymphocytes/IMMUNOLOGY  Female  Gene Rearrangement,
       beta-Chain T-Cell Antigen Receptor  Human  HLA-DR Antigens/*METABOLISM
       Lymphocyte Transformation  Male  Middle Age  Nucleosidases/*METABOLISM
       Receptors, Antigen, T-Cell, alpha-beta/*GENETICS  Support, Non-U.S.
       Gov't  T-Lymphocyte Subsets/*IMMUNOLOGY  Zidovudine/THERAPEUTIC USE
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

