       Document 0526
 DOCN  M9610526
 TI    Crohn's disease is accompanied by changes in the CD4+, but not CD8+, T
       cell receptor BV repertoire of lamina propria lymphocytes.
 DT    9601
 AU    Gulwani-Akolkar B; Akolkar PN; McKinley M; Fisher SE; Silver J;
       Department of Medicine, North Shore University Hospital/Cornell;
       University Medical College, Manhasset, New York 11030, USA.
 SO    Clin Immunol Immunopathol. 1995 Oct;77(1):95-106. Unique Identifier :
       AIDSLINE MED/96010059
 AB    To identify disease-specific T cell changes that occur in Crohn's
       disease (CD), the T cell receptor (TCR) BV repertoires of lamina propria
       lymphocytes (LPL) isolated from the diseased colon of seven CD patients
       and eight controls were determined by semiquantitative polymerase chain
       reaction (qPCR). As an internal control for the effects of HLA and other
       genes on the TCR repertoire, the BV repertoires of peripheral blood
       lymphocytes (PBL) from the same individuals were similarly determined
       and used for comparison. It was observed that the BV repertoires of LPL
       and PBL within the same individual were very different in both the CD
       and control groups. However, the CD4+, but not CD8+, repertoires of LPL
       and PBL differed to a much greater extent in the CD group than in the
       control group. Furthermore, in each CD patient there was a unique
       pattern of BV segments which were increased in the CD4+ LPL repertoire
       relative to that in PBL. These observations suggest that the
       inflammatory process in CD involves responses by specific CD4+ T cells
       to specific antigens. The isolation of such inflammation-specific CD4+ T
       cells may make it possible to identify the antigens which are
       responsible for the inflammatory process in CD and provide a better
       understanding of its pathogenesis.
 DE    Adolescence  Adult  Crohn Disease/*IMMUNOLOGY  CD4-Positive
       T-Lymphocytes/*IMMUNOLOGY  CD8-Positive T-Lymphocytes/*IMMUNOLOGY  Gene
       Expression  Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
       Human  Intestines/*IMMUNOLOGY  Middle Age  Receptors, Antigen, T-Cell,
       alpha-beta/GENETICS/*IMMUNOLOGY  RNA, Messenger/GENETICS  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Twins, Monozygotic  JOURNAL
       ARTICLE  TWIN STUDY

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

