       Document 0521
 DOCN  M9610521
 TI    Laboratory and clinical evaluation of Mycobacterium xenopi isolates.
 DT    9601
 AU    Marx CE; Fan K; Morris AJ; Wilson ML; Damiani A; Weinstein MP; Clinical
       Microbiology Laboratory, Duke University Medical Center,; Durham, North
       Carolina, USA.
 SO    Diagn Microbiol Infect Dis. 1995 Apr;21(4):195-202. Unique Identifier :
       AIDSLINE MED/96055591
 AB    Mycobacterium xenopi and Mycobacterium avium complex (MAC) are
       biochemically similar. To define the laboratory characteristics of M.
       xenopi that distinguish it from MAC, 53 M. xenopi isolates from
       different areas in the United States and 47 isolates recovered at one
       hospital were evaluated by 13 biochemical tests, AccuProbe MAC
       (Gen-Probe, Inc., San Diego, CA, USA), colony morphology, formation of
       X-colonies, pigmentation in response to light, growth on MacConkey agar
       without crystal violet, and relative growth rates at 25 degrees C, 36
       degrees C, and 45 degrees C on solid media. Relative growth rates of 10
       M. xenopi and 11 MAC isolates were measured at 25 degrees C, 36 degrees
       C, and 42 degrees C in Middlebrook broth processed using the BACTEC TB
       System. Ten M. xenopi were tested for
       p-nitro-alpha-acetylamino-beta-hydroxypropiophenone inhibition at 36
       degrees C and 42 degrees C. Reevaluation of 81 isolates previously
       identified as MAC by biochemical tests alone revealed that two were M.
       xenopi. The most reliable characteristics distinguishing M. xenopi from
       MAC were the presence of X-colonies (M. xenopi 97% vs MAC 1%), positive
       3-day arylsulfatase (M. xenopi 88% vs MAC 1%), growth at 25 degrees C
       (M. xenopi 0% vs MAC 100%), and AccuProbe MAC test results (M. xenopi 0%
       hybridized). Retrospective chart review of 37 patients using American
       Thoracic Society criteria revealed that six (16%) patients had
       clinically important isolates. At one of our hospitals M. xenopi was the
       second most common mycobacterial species isolated for 1990-1992,
       accounting for 27% of all isolates, whereas at our other hospital it
       accounted for 1% of isolates.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Adult  Aged  AIDS-Related Opportunistic Infections/*MICROBIOLOGY
       Bacteriological Techniques  Colony Count, Microbial  Female  Human  Male
       Middle Age  Mycobacterium/*CLASSIFICATION/GROWTH & DEVELOPMENT/ISOLATION
       &  PURIF  Mycobacterium avium Complex/GROWTH & DEVELOPMENT/*ISOLATION &
       PURIF  Mycobacterium avium-intracellulare Infection/*MICROBIOLOGY
       JOURNAL ARTICLE  MULTICENTER STUDY

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

