       Document 0497
 DOCN  M9610497
 TI    Intravital demonstration of sequential migration process of lymphocyte
       subpopulations in rat Peyer's patches.
 DT    9601
 AU    Miura S; Tsuzuki Y; Fukumura D; Serizawa H; Suematsu M; Kurose I; Imaeda
       H; Kimura H; Nagata H; Tsuchiya M; et al; Department of Medicine, School
       of Medicine, Keio University,; Tokyo, Japan.
 SO    Gastroenterology. 1995 Oct;109(4):1113-23. Unique Identifier : AIDSLINE
       MED/96029311
 AB    BACKGROUND & AIMS: Although recirculation of lymphocytes through Peyer's
       patches is important for specific immune defense, the intraorgan
       migration of lymphocyte subpopulations has not been clearly understood.
       The aim of this study was to compare the spatial distributions of
       labeled lymphocytes among various subpopulations in rat Peyer's patches.
       METHODS: Lymphocytes collected from intestinal lymph were separated into
       CD4+, CD8+, and T and B cells, labeled with a fluorochrome
       carboxyfluorescein diacetate succinimidyl ester, and injected into the
       jugular vein. Peyer's patches of recipient rats were observed by
       intravital fluorescence microscopy. RESULTS: No significant difference
       was found in the percentage of lymphocytes in transit or in the rolling
       velocity among different subpopulations. Lymphocytes sticking to the
       venules increased in number at 10-20 minutes, with preferential
       adherence of CD4+ cells to venules of 25-50 microns and preferential
       adherence of B cells to the venules of a wider size range. After 30
       minutes, extravasated lymphocytes moved into the interstitium. B cells
       migrated from venules more quickly than CD4+ cells. CD8+ cells showed an
       intermediate pattern between CD4+ and B cells in sticking and migratory
       behaviors. Subsequently, CD4+ and CD8 cells preferentially appeared in
       parafollicular microlymphatics. CONCLUSIONS: Significant differences
       were observed among lymphocyte subpopulations in terms of spatial
       distribution of lymphocytes sticking to venules, migration into the
       interstitium, and their lymphatic transport.
 DE    Animal  B-Lymphocytes/IMMUNOLOGY  Cell Movement  Cell Separation
       CD4-Positive T-Lymphocytes/IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  Lymphocyte Subsets/*IMMUNOLOGY/PHYSIOLOGY
       Male  Microscopy, Fluorescence  Peyer's Patches/CYTOLOGY/*IMMUNOLOGY
       Rats  Rats, Wistar  Support, Non-U.S. Gov't  T-Lymphocytes/IMMUNOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

