       Document 0490
 DOCN  M9610490
 TI    Stress- and sequence-dependent release into the culture medium of HIV-1
       Nef produced in Saccharomyces cerevisiae.
 DT    9601
 AU    Macreadie IG; Castelli LA; Lucantoni A; Azad AA; Biomolecular Research
       Institute, Parkville, Victoria, Australia.
 SO    Gene. 1995 Sep 11;162(2):239-43. Unique Identifier : AIDSLINE
       MED/96032350
 AB    We have produced human immunodeficiency virus type 1 (HIV-1) Nef (a
       myristylated 206-amino-acid protein) in Saccharomyces cerevisaie and
       shown that, while Nef is normally found as a predominantly intracellular
       protein, amounts up to 40 micrograms/ml of Nef are also released into
       the extracellular medium during stress. By electrophoretic (SDS-PAGE)
       analysis the extracellular Nef is indistinguishable from intracellular
       Nef. Conditions of stress that lead to the release of Nef include
       elevated levels of copper or magnesium ions or growth at elevated
       temperatures. This release appears to be dependent upon the N-terminal
       sequences of Nef, including the presence of a myristylation site. Our
       observations concerning Nef release in yeast suggest new ways in which
       the behaviour of Nef should be examined in order to gain further
       insights into the development of AIDS. If the release of Nef is
       important in the development of AIDS, our work reveals that
       Nef-associated symptoms may be reduced or delayed by reducing stresses,
       such as fevers.
 DE    Amino Acid Sequence  Base Sequence  Copper/PHARMACOLOGY  Gene Products,
       nef/CHEMISTRY/*SECRETION  Heat  HIV-1/*CHEMISTRY  Magnesium/PHARMACOLOGY
       Molecular Sequence Data  Myristic Acids/METABOLISM  Protein Processing,
       Post-Translational  Recombinant Proteins/*SECRETION  Saccharomyces
       cerevisiae/*METABOLISM  Stress/METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

