       Document 0479
 DOCN  M9610479
 TI    Differential activation of mouse hepatitis virus-specific CD4+ cytotoxic
       T cells is defined by peptide length.
 DT    9601
 AU    Heemskerk MH; Schoemaker HM; De Jong I; Schijns VE; Spaan WJ; Boog CJ;
       Department of Immunology, Faculty of Veterinary Medicine, Utrecht;
       University, The Netherlands.
 SO    Immunology. 1995 Aug;85(4):517-22. Unique Identifier : AIDSLINE
       MED/96030662
 AB    In this study we have characterized the core epitope recognized by the
       MHV-A59-specific CD4+ cytotoxic T lymphocyte (CTL) clones HS1 and B6.1,
       derived from BALB/c and C57/BL6 mice, respectively. These CD4+ clones
       respond to the promiscuous peptide fragment S-329-343 of the
       glycoprotein S of MHV-A59. The results indicate that the core peptides
       of both clones overlap but are not identical. The core region of the HS1
       clone is an 8-mer, and comprises the amino acid residues S-332-339,
       whereas the minimal epitope for clone B6.1 is a 9-mer and comprises the
       amino acid residues S-334-342. The peptide fragment S-329-343 activates
       all T-cell effector functions, including proliferation, cytokine
       secretion and cytolysis. However, in the present study we show that
       T-cell activation is not an all-or-none phenomenon, in which T-cell
       stimulation leads to activation of all T-cell effector functions. It
       appears that changes in the length of a peptide ligand can
       differentially activate the cytolytic machinery from proliferation and
       cytokine secretion. Furthermore, the results indicate that, in our case,
       modulation of the flanking residues of the core epitopes did not convert
       the cytokine profile of polarized T-helper type-1 (Th1) clones into a
       Th2-type pattern.
 DE    Amino Acid Sequence  Animal  Antigens, Viral/*IMMUNOLOGY  Cell
       Division/IMMUNOLOGY  Clone Cells/IMMUNOLOGY  Cytokines/BIOSYNTHESIS
       Cytotoxicity, Immunologic  CD4-Positive T-Lymphocytes/*IMMUNOLOGY
       Epitopes/IMMUNOLOGY  Gastroenteritis Virus, Murine/*IMMUNOLOGY
       Lymphocyte Transformation/*IMMUNOLOGY  Mice  Mice, Inbred BALB C  Mice,
       Inbred C57BL  Molecular Sequence Data  Peptide Fragments/*IMMUNOLOGY
       Support, Non-U.S. Gov't  Th1 Cells/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

