       Document 0477
 DOCN  M9610477
 TI    Different migration patterns of antigen-presenting cells correlate with
       Th1/Th2-type responses in mice.
 DT    9601
 AU    Morikawa Y; Tohya K; Ishida H; Matsuura N; Kakudo K; Department of
       Pathology, Wakayama Medical School, Japan.
 SO    Immunology. 1995 Aug;85(4):575-81. Unique Identifier : AIDSLINE
       MED/96030671
 AB    Antibodies are produced when antigen-presenting cells (APC) pulsed with
       an antigen are injected intravenously (i.v.) into BALB/c mice, but
       subcutaneous (s.c.) injection of such APC causes delayed-type
       hypersensitivity (DTH). To identify the anatomic sites where T and B
       cells are activated, we labelled splenic dendritic cells (DC) with a
       fluorochrome, PKH 26, injected them i.v. or s.c., and used the label to
       locate them. When the DC were injected i.v., germinal centres in the
       spleen were hyperplastic on day 1. Most DC moved to T-dependent areas of
       the white and red pulp on day 1 and remained there at least until day 5,
       but no DC migrated into the lymph nodes. When the DC were injected s.c.,
       they were in the sinus on day 1 and had entered T-dependent area of
       draining lymph nodes only by day 3; hyperplasia of germinal centres in
       the spleen and migration of DC into the spleen were not found. We used
       the polymerase chain reaction (PCR) to study which mice had spleen cells
       and lymph node cells that produced the cytokines interleukin (IL)-2,
       IL-4, IL-10, and interferon-gamma (IFN-gamma). In the sensitization
       phase, day 1 after DC injection i.v., almost all IL-10 transcript was
       found in spleen cells, but after DC injection s.c., IL-2 message was
       most abundant in lymph node cells. The expression of mRNA for IL-4 and
       IFN-gamma in mice that received DC i.v. was not different from that in
       mice that received DC s.c. in this phase. Immunohistochemical staining
       showed that cells stained for IL-10 were in the T-dependent area of the
       spleen from mice that received DC i.v. 1 day after the injection. Three
       days after the injection of DC i.v., cells stained for IL-10 were in the
       germinal centres as well. The number of such cells in the spleen of mice
       that received DC i.v. was significantly more than that in mice that
       received DC s.c. IL-10 may be important in development of TH2 response.
 DE    Animal  Base Sequence  Cell Movement/IMMUNOLOGY  Dendritic
       Cells/*IMMUNOLOGY  Female  Hyperplasia/PATHOLOGY
       Interleukin-10/BIOSYNTHESIS  Lymph Nodes/PATHOLOGY  Mice  Mice, Inbred
       BALB C  Molecular Sequence Data  Polymerase Chain Reaction
       Spleen/IMMUNOLOGY/PATHOLOGY  Support, Non-U.S. Gov't  Th1
       Cells/*IMMUNOLOGY  Th2 Cells/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

