       Document 0476
 DOCN  M9610476
 TI    Differential antibody isotype reactivity to specific antigens in human
       lymphatic filariasis: gp15/400 preferentially induces immunoglobulin E
       (IgE), IgG4, and IgG2.
 DT    9601
 AU    Yazdanbakhsh M; Paxton WA; Brandenburg A; Van Ree R; Lens M; Partono F;
       Maizels RM; Selkirk ME; Department of Parasitology, University of
       Leiden, The; Netherlands.
 SO    Infect Immun. 1995 Oct;63(10):3772-9. Unique Identifier : AIDSLINE
       MED/96009726
 AB    Lymphatic filarial infection in humans is associated with a strong
       skewing of the immune response towards the TH2 arm, with prominent
       interleukin 4-producing cells and elevated levels of immunoglobulin G4
       (IgG4) and IgE antibodies in peripheral blood. To determine how such a
       generalized TH2 imbalance governs responses to individual parasite
       antigens, the profiles of isotypes of antibodies to two recombinant
       proteins of Brugia spp. were studied. One molecule was the C-terminal
       portion of the filarial heat shock protein 70 (Bpa-26), representative
       of a cytoplasmic protein, and the second antigen was a single unit of
       the tandem repeats of a Brugia polypeptide (BpL-4), a secreted product
       which is prominently exposed to the immune system. Serum samples from
       146 individuals resident in areas in which brugian filariasis is endemic
       were used, and it was found that whereas the levels of IgG1 and IgG3
       responses to both Bpa-26 and BpL-4 were high, IgG4 and IgE antibodies to
       only BpL-4, not to Bpa-26, were prominent. Thus, an antigen which is
       chronically exposed to the immune system elicited a TH2-dependent
       isotype switch, as manifested by increased IgG4 and IgE responses.
       Moreover, IgG4 and IgE responses to BpL-4 showed a strong negative
       association, suggesting that mediators other than interleukin 4 must be
       responsible for such differential regulation of these two isotypes. When
       the data were analyzed as a function of clinical status, a striking
       association between elevated levels of IgG3 antibodies to Bpa-26 and
       manifestation of chronic obstructive disease was found; elephantiasis
       patients showed significantly higher levels of IgG3 antibodies to Bpa-26
       than microfilaremics and asymptomatic amicrofilaremics. This indicates
       that an imbalance of isotypes of antibodies to particular filarial
       antigens might play a role in the pathogenesis of chronic disease.
 DE    Adolescence  Adult  Aged  Animal  Antibodies, Helminth/*BIOSYNTHESIS
       Antigens, Helminth/*IMMUNOLOGY  Brugia/*IMMUNOLOGY  Elephantiasis,
       Filarial/*IMMUNOLOGY  Female  Helminth Proteins/*IMMUNOLOGY  Human
       IgE/BIOSYNTHESIS  IgG/BIOSYNTHESIS/CLASSIFICATION  Immunoglobulin
       Isotypes/*BIOSYNTHESIS  Male  Middle Age  Support, Non-U.S. Gov't  Th1
       Cells/IMMUNOLOGY  Th2 Cells/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

