       Document 0473
 DOCN  M9610473
 TI    T-cell suppression and selective in vivo activation of TH2 subpopulation
       by the Entamoeba histolytica 220-kilodalton lectin.
 DT    9601
 AU    Talamas-Rohana P; Schlie-Guzman MA; Hernandez-Ramirez VI; Rosales-Encina
       JL; Department of Experimental Pathology, Centro de Investigacion y; de
       Estudios Avanzados del Instituto Politecnico Nacional, Mexico; City,
       Mexico.
 SO    Infect Immun. 1995 Oct;63(10):3953-8. Unique Identifier : AIDSLINE
       MED/96009751
 AB    A 220-kDa surface protein (L220) with lectin activity from Entamoeba
       histolytica trophozoites has been characterized previously (J. L.
       Rosales-Encina, I. Meza, A. Lopez-de-Leon, P. Talamas-Rohana, and M.
       Rojkind, J. Infect. Dis. 156:790-797, 1987). This molecule is involved
       in the adhesion process (I. Meza, F. Cazares, J. L. Rosales-Encina, P.
       Talamas-Rohana, and M. Rojkind, J. Infect. Dis. 156:798-805, 1987) and
       is very immunogenic. In this work, we studied both the humoral and the
       cellular immune responses to L220. We compared L220 with L220-derived
       components, such as a fusion peptide (M-11) and chemically obtained
       peptides (by treating the 220-kDa molecule with
       N-chlorosuccinimide-urea). Spleen cells from L220-immunized mice were
       unable to proliferate in vitro when stimulated with the protein.
       However, a proliferative response was obtained when mice were immunized
       with the L220-derived fusion peptide or the cleaved lectin. To find out
       if there was a correlation between the observed responses and TH1 or TH2
       activation, we analyzed patterns of cytokine secretion (interleukin-2
       [IL-2], IL-4, IL-10, and gamma interferon). Cells from mice immunized
       with peptides that induced cell proliferation (100, 80, and 47 kDa) with
       the peptides (P < 0.01) and with the intact molecule secreted IL-2 and
       gamma interferon, showing a TH1-subset pattern. Conversely, cells from
       mice immunized with the intact 220-kDa molecule secreted IL-4 and IL-10,
       typical of a TH2 subpopulation; however, antibodies from each group
       recognized the 220-kDa molecule as determined by Western blotting
       (immunoblotting). These results suggest that various epitopes in the
       220-kDa molecule generate different response patterns, suppressing or
       activating T-cell responses.
 DE    Animal  Cytokines/BIOSYNTHESIS  Entamoeba histolytica/*IMMUNOLOGY
       Immunization  Lectins/*IMMUNOLOGY  *Lymphocyte Transformation  Mice
       Mice, Inbred BALB C  Molecular Weight  Protozoan Proteins/*IMMUNOLOGY
       Support, Non-U.S. Gov't  Th1 Cells/IMMUNOLOGY  Th2 Cells/*IMMUNOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

