       Document 0471
 DOCN  M9610471
 TI    Lack of a role for natural killer cells in early control of Brucella
       abortus 2308 infections in mice.
 DT    9601
 AU    Fernandes DM; Benson R; Baldwin CL; Department of Veterinary and Animal
       Sciences, University of; Massachusetts, Amherst 01003, USA.
 SO    Infect Immun. 1995 Oct;63(10):4029-33. Unique Identifier : AIDSLINE
       MED/96009762
 AB    Studies were conducted to determine if natural killer (NK) cells are
       important for early control of the virulent strain Brucella abortus 2308
       following infection of mice with high or low challenge doses.
       Splenocytes from C57BL/10 and BALB/c mice that had been infected with
       the lower dose of B. abortus displayed increased cytotoxicity against
       YAC-1 cells during the first week after infection, while infection of
       C57BL/10 mice with the higher challenge dose either did not alter the
       level of NK cytotoxic activity or decreased it, depending upon the time
       postinfection. In vivo depletion of NK cells by monoclonal antibody
       anti-NK1.1 or polyclonal anti-asialoGM1 antiserum did not result in an
       increase in the number of brucellae recovered from the spleens or livers
       of the brucella-resistant C57BL/10 mice or from the spleens of the
       susceptible BALB/c mice during the first week after infection. Treatment
       of control mice with the NK-reactive antibodies, however, decreased
       killing of the NK-sensitive target YAC-1, indicating that the NK cell
       depletion regimes were effective. Our results suggest that NK cells are
       not crucial for early control of B. abortus 2308 even though they may be
       activated following infection. Further experiments indicated that
       treatment of C57BL/10 mice with poly(A:U) did not decrease the number of
       brucellae recovered from their spleens although it did decrease the CFU
       in livers of mice infected with the high challenge dose.
 DE    Animal  Brucella abortus/*IMMUNOLOGY  Brucellosis/*IMMUNOLOGY  Female
       Killer Cells, Natural/*IMMUNOLOGY  Lymphocyte Transformation  Mice
       Mice, Inbred BALB C  Mice, Inbred C57BL  Support, U.S. Gov't, Non-P.H.S.
       Th1 Cells/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

