       Document 0448
 DOCN  M9610448
 TI    CD8+ T lymphocytes are recruited to neoplastic cervix.
 DT    9601
 AU    Bell MC; Edwards RP; Partridge EE; Kuykendall K; Conner W; Gore H;
       Turbat-Herrara E; Crowley-Nowick PA; Department of OB/GYN, University of
       Alabama at Birmingham 35233,; USA.
 SO    J Clin Immunol. 1995 May;15(3):130-6. Unique Identifier : AIDSLINE
       MED/96053530
 AB    ToliicIV distinguish normal cervical lymphocyte populations from
       phenotypes recruited to the cervix in response to cervical neoplasia,
       lymphocytes were isolated from normal and neoplastic cervix. A portion
       of the cervical transformation zone was obtained from 19 patients with
       pathologically confirmed cervical intraepithelial neoplasia and from 20
       patients with normal cervices undergoing hysterectomy for benign
       indications. Mononuclear cells were harvested from cervical tissue using
       a serial, multienzymatic digestion procedure and enriched by density
       gradient centrifugation. Isolated cell populations were stained with
       surface marker-specific monoclonal antibodies and analyzed by
       fluorescent activated cell sorter to determine the percentage of B
       cells, total T cells, CD4+ T cells, CD8+ T cells, and natural killer
       (NK) cells. The distribution of circulating peripheral blood lymphocyte
       phenotypes was similar for both patients with neoplasia and normal
       controls. A marked disparity in the proportions of NK cells and T cells
       was demonstrated among lymphocyte phenotypes infiltrating the cervix.
       The percentage of CD4+ T cells and NK cells was significantly depressed
       (P = 0.04, P = 0.03, respectively) in dysplastic tissue as compared to
       normal cervical tissue. In contrast, the proportion of CD8+ T cells was
       significantly increased in the dysplastic tissue (P = 0.0001). Analysis
       of immunocompetent cells in the circulation appears to have little
       correlation with immunocytes present in the dysplastic epithelium. The
       depression in the proportion of CD4+ T lymphocytes and NK cells at the
       cervical squamocolumnar junction reflects a local recruitment of CD8+ T
       cells to the site of neoplasia in the cervix.
 DE    Adolescence  Adult  Carcinoma in Situ/*IMMUNOLOGY/PATHOLOGY  Carcinoma,
       Squamous Cell/*IMMUNOLOGY/PATHOLOGY  Cell Separation  Cervix
       Neoplasms/*IMMUNOLOGY/PATHOLOGY  Cervix Uteri/*IMMUNOLOGY/PATHOLOGY
       CD8-Positive T-Lymphocytes/*IMMUNOLOGY  Female  Human  Hysterectomy
       Immunophenotyping  Lymphocyte Count  Lymphocytes,
       Tumor-Infiltrating/*IMMUNOLOGY/PATHOLOGY  Middle Age  Support, Non-U.S.
       Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

